Limits...
Visible rodent brain-wide networks at single-neuron resolution.

Yuan J, Gong H, Li A, Li X, Chen S, Zeng S, Luo Q - Front Neuroanat (2015)

Bottom Line: There are some unsolvable fundamental questions, such as cell type classification, neural circuit tracing and neurovascular coupling, though great progresses are being made in neuroscience.Because of the structural features of neurons and neural circuits, the solution of these questions needs us to break through the current technology of neuroanatomy for acquiring the exactly fine morphology of neuron and vessels and tracing long-distant circuit at axonal resolution in the whole brain of mammals.Combined with fast-developing labeling techniques, efficient whole-brain optical imaging technology emerging at the right moment presents a huge potential in the structure and function research of specific-function neuron and neural circuit.

View Article: PubMed Central - PubMed

Affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology Wuhan, China ; Key Laboratory of Biomedical Photonics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.

ABSTRACT
There are some unsolvable fundamental questions, such as cell type classification, neural circuit tracing and neurovascular coupling, though great progresses are being made in neuroscience. Because of the structural features of neurons and neural circuits, the solution of these questions needs us to break through the current technology of neuroanatomy for acquiring the exactly fine morphology of neuron and vessels and tracing long-distant circuit at axonal resolution in the whole brain of mammals. Combined with fast-developing labeling techniques, efficient whole-brain optical imaging technology emerging at the right moment presents a huge potential in the structure and function research of specific-function neuron and neural circuit. In this review, we summarize brain-wide optical tomography techniques, review the progress on visible brain neuronal/vascular networks benefit from these novel techniques, and prospect the future technical development.

No MeSH data available.


The reconstruction of the coronal plane imaging (300 μm thick) of GFP-M line mouse brain using fMOST. A 3D reconstruction of the mouse brain is in the top left corner; the cube at the coronal plane presents the spatial location of the data at the center. Six individual pyramidal neurons were segmented and traced, indicated in different colors. Axon arbors extend in the ipsilateral hemisphere and to the caudate putamen in the contralateral hemisphere via the corpus callosum. Redrawn from the imaging dataset published in Gong et al. (2013).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4446545&req=5

Figure 3: The reconstruction of the coronal plane imaging (300 μm thick) of GFP-M line mouse brain using fMOST. A 3D reconstruction of the mouse brain is in the top left corner; the cube at the coronal plane presents the spatial location of the data at the center. Six individual pyramidal neurons were segmented and traced, indicated in different colors. Axon arbors extend in the ipsilateral hemisphere and to the caudate putamen in the contralateral hemisphere via the corpus callosum. Redrawn from the imaging dataset published in Gong et al. (2013).

Mentions: In 2013, the fMOST method had demonstrated the first long-range tracing of individual axons in the mouse brain (Gong et al., 2013; Osten and Margrie, 2013). These findings not only confirmed previously discovered pathways but also confirmed several unreported, putative projection pathways. Thus far, this has been a valuable technique to provide sufficiently intricate data describing the neural circuits in an arbitrary region of the mouse brain. A representative projection pattern of homotopic axons originating from the primary motor cortex is shown in Figure 3. Hopefully, the combination of fMOST and newly developed trans-synaptic tracing methods will provide new insight into acquiring long-range and trans-synaptic tracking of specific-labeled neural circuits. Without doubt, these methods will accelerate the characterization of information flow in brain circuits.


Visible rodent brain-wide networks at single-neuron resolution.

Yuan J, Gong H, Li A, Li X, Chen S, Zeng S, Luo Q - Front Neuroanat (2015)

The reconstruction of the coronal plane imaging (300 μm thick) of GFP-M line mouse brain using fMOST. A 3D reconstruction of the mouse brain is in the top left corner; the cube at the coronal plane presents the spatial location of the data at the center. Six individual pyramidal neurons were segmented and traced, indicated in different colors. Axon arbors extend in the ipsilateral hemisphere and to the caudate putamen in the contralateral hemisphere via the corpus callosum. Redrawn from the imaging dataset published in Gong et al. (2013).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4446545&req=5

Figure 3: The reconstruction of the coronal plane imaging (300 μm thick) of GFP-M line mouse brain using fMOST. A 3D reconstruction of the mouse brain is in the top left corner; the cube at the coronal plane presents the spatial location of the data at the center. Six individual pyramidal neurons were segmented and traced, indicated in different colors. Axon arbors extend in the ipsilateral hemisphere and to the caudate putamen in the contralateral hemisphere via the corpus callosum. Redrawn from the imaging dataset published in Gong et al. (2013).
Mentions: In 2013, the fMOST method had demonstrated the first long-range tracing of individual axons in the mouse brain (Gong et al., 2013; Osten and Margrie, 2013). These findings not only confirmed previously discovered pathways but also confirmed several unreported, putative projection pathways. Thus far, this has been a valuable technique to provide sufficiently intricate data describing the neural circuits in an arbitrary region of the mouse brain. A representative projection pattern of homotopic axons originating from the primary motor cortex is shown in Figure 3. Hopefully, the combination of fMOST and newly developed trans-synaptic tracing methods will provide new insight into acquiring long-range and trans-synaptic tracking of specific-labeled neural circuits. Without doubt, these methods will accelerate the characterization of information flow in brain circuits.

Bottom Line: There are some unsolvable fundamental questions, such as cell type classification, neural circuit tracing and neurovascular coupling, though great progresses are being made in neuroscience.Because of the structural features of neurons and neural circuits, the solution of these questions needs us to break through the current technology of neuroanatomy for acquiring the exactly fine morphology of neuron and vessels and tracing long-distant circuit at axonal resolution in the whole brain of mammals.Combined with fast-developing labeling techniques, efficient whole-brain optical imaging technology emerging at the right moment presents a huge potential in the structure and function research of specific-function neuron and neural circuit.

View Article: PubMed Central - PubMed

Affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology Wuhan, China ; Key Laboratory of Biomedical Photonics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.

ABSTRACT
There are some unsolvable fundamental questions, such as cell type classification, neural circuit tracing and neurovascular coupling, though great progresses are being made in neuroscience. Because of the structural features of neurons and neural circuits, the solution of these questions needs us to break through the current technology of neuroanatomy for acquiring the exactly fine morphology of neuron and vessels and tracing long-distant circuit at axonal resolution in the whole brain of mammals. Combined with fast-developing labeling techniques, efficient whole-brain optical imaging technology emerging at the right moment presents a huge potential in the structure and function research of specific-function neuron and neural circuit. In this review, we summarize brain-wide optical tomography techniques, review the progress on visible brain neuronal/vascular networks benefit from these novel techniques, and prospect the future technical development.

No MeSH data available.