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Elevated air movement enhances stomatal sensitivity to abscisic acid in leaves developed at high relative air humidity.

Carvalho DR, Torre S, Kraniotis D, Almeida DP, Heuvelink E, Carvalho SM - Front Plant Sci (2015)

Bottom Line: Endogenous concentration of ABA and its metabolites in the leaves was reduced by 35% in high RH, but contrary to our hypothesis this concentration was not significantly affected by high MOV.Interestingly, in detached leaflets grown at high RH, high MOV increased stomatal sensitivity to ABA since the amount of exogenous ABA required to decrease the transpiration rate was significantly reduced.This is the first study to show that high MOV increases stomatal functionality in leaves developed at high RH by reducing the stomatal pore length and aperture and enhancing stomatal sensitivity to ABA rather than increasing leaf [ABA].

View Article: PubMed Central - PubMed

Affiliation: Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa Porto, Portugal.

ABSTRACT
High relative air humidity (RH ≥ 85%) during growth leads to stomata malfunctioning, resulting in water stress when plants are transferred to conditions of high evaporative demand. In this study, we hypothesized that an elevated air movement (MOV) 24 h per day, during the whole period of leaf development would increase abscisic acid concentration ([ABA]) enhancing stomatal functioning. Pot rose 'Toril' was grown at moderate (61%) or high (92%) RH combined with a continuous low (0.08 m s(-1)) or high (0.92 m s(-1)) MOV. High MOV reduced stomatal pore length and aperture in plants developed at high RH. Moreover, stomatal function improved when high MOV-treated plants were subjected to leaflet desiccation and ABA feeding. Endogenous concentration of ABA and its metabolites in the leaves was reduced by 35% in high RH, but contrary to our hypothesis this concentration was not significantly affected by high MOV. Interestingly, in detached leaflets grown at high RH, high MOV increased stomatal sensitivity to ABA since the amount of exogenous ABA required to decrease the transpiration rate was significantly reduced. This is the first study to show that high MOV increases stomatal functionality in leaves developed at high RH by reducing the stomatal pore length and aperture and enhancing stomatal sensitivity to ABA rather than increasing leaf [ABA].

No MeSH data available.


Related in: MedlinePlus

Effect of moderate (61%; open columns) and high (92%; solid columns) RH (A–E), combined with no additional MOV (light gray columns) or with additional MOV (gray columns; F–J) on the levels (μg g-1 DW) of ABA (A,F), PA (B,G), DPA (C,H), ABA-GE (D,I) and total concentration of ABA and its metabolites (E,J) in leaves of rose plants cv. ‘Toril.’ Sampling occurred 5 h after the beginning of the light period. Each sample consisted of a composite of seven leaflets from seven biological replicates. Values are the mean of two biological repeats and bars represent the SEM. Different letters indicate significant differences according to Fisher’s LSD test (P = 0.05).
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Figure 4: Effect of moderate (61%; open columns) and high (92%; solid columns) RH (A–E), combined with no additional MOV (light gray columns) or with additional MOV (gray columns; F–J) on the levels (μg g-1 DW) of ABA (A,F), PA (B,G), DPA (C,H), ABA-GE (D,I) and total concentration of ABA and its metabolites (E,J) in leaves of rose plants cv. ‘Toril.’ Sampling occurred 5 h after the beginning of the light period. Each sample consisted of a composite of seven leaflets from seven biological replicates. Values are the mean of two biological repeats and bars represent the SEM. Different letters indicate significant differences according to Fisher’s LSD test (P = 0.05).

Mentions: Abscisic acid and its metabolites (PA, DPA, ABA-GE, 7′OH-ABA, neoPA, trans-ABA, and cis-ABA) were quantified in fully developed leaves. In all treatments, the levels of 7′OH-ABA, neoPA, trans-ABA, and cis-ABA were very low (data not shown), hence, they have only been included in the combined amount of ABA and its metabolites when quantification was possible. High RH reduced the concentrations of ABA by 52% (P = 0.005; Figure 4A), PA by 46% (P = 0.008; Figure 4B), DPA by 48% (P = 0.004; Figure 4C), ABA-GE by 23% (P = 0.184; Figure 4D) and the combination of ABA and its metabolites by 35% (P = 0.013; Figure 4E). High MOV did not significantly affect the concentration of ABA (P = 0.764, Figure 4F), PA (P = 0.224, Figure 4G), DPA (P = 0.234, Figure 4H), ABA-GE (P = 0.488, Figure 4I), or combined ABA and its metabolites (P = 0.671, Figure 4J).


Elevated air movement enhances stomatal sensitivity to abscisic acid in leaves developed at high relative air humidity.

Carvalho DR, Torre S, Kraniotis D, Almeida DP, Heuvelink E, Carvalho SM - Front Plant Sci (2015)

Effect of moderate (61%; open columns) and high (92%; solid columns) RH (A–E), combined with no additional MOV (light gray columns) or with additional MOV (gray columns; F–J) on the levels (μg g-1 DW) of ABA (A,F), PA (B,G), DPA (C,H), ABA-GE (D,I) and total concentration of ABA and its metabolites (E,J) in leaves of rose plants cv. ‘Toril.’ Sampling occurred 5 h after the beginning of the light period. Each sample consisted of a composite of seven leaflets from seven biological replicates. Values are the mean of two biological repeats and bars represent the SEM. Different letters indicate significant differences according to Fisher’s LSD test (P = 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4446533&req=5

Figure 4: Effect of moderate (61%; open columns) and high (92%; solid columns) RH (A–E), combined with no additional MOV (light gray columns) or with additional MOV (gray columns; F–J) on the levels (μg g-1 DW) of ABA (A,F), PA (B,G), DPA (C,H), ABA-GE (D,I) and total concentration of ABA and its metabolites (E,J) in leaves of rose plants cv. ‘Toril.’ Sampling occurred 5 h after the beginning of the light period. Each sample consisted of a composite of seven leaflets from seven biological replicates. Values are the mean of two biological repeats and bars represent the SEM. Different letters indicate significant differences according to Fisher’s LSD test (P = 0.05).
Mentions: Abscisic acid and its metabolites (PA, DPA, ABA-GE, 7′OH-ABA, neoPA, trans-ABA, and cis-ABA) were quantified in fully developed leaves. In all treatments, the levels of 7′OH-ABA, neoPA, trans-ABA, and cis-ABA were very low (data not shown), hence, they have only been included in the combined amount of ABA and its metabolites when quantification was possible. High RH reduced the concentrations of ABA by 52% (P = 0.005; Figure 4A), PA by 46% (P = 0.008; Figure 4B), DPA by 48% (P = 0.004; Figure 4C), ABA-GE by 23% (P = 0.184; Figure 4D) and the combination of ABA and its metabolites by 35% (P = 0.013; Figure 4E). High MOV did not significantly affect the concentration of ABA (P = 0.764, Figure 4F), PA (P = 0.224, Figure 4G), DPA (P = 0.234, Figure 4H), ABA-GE (P = 0.488, Figure 4I), or combined ABA and its metabolites (P = 0.671, Figure 4J).

Bottom Line: Endogenous concentration of ABA and its metabolites in the leaves was reduced by 35% in high RH, but contrary to our hypothesis this concentration was not significantly affected by high MOV.Interestingly, in detached leaflets grown at high RH, high MOV increased stomatal sensitivity to ABA since the amount of exogenous ABA required to decrease the transpiration rate was significantly reduced.This is the first study to show that high MOV increases stomatal functionality in leaves developed at high RH by reducing the stomatal pore length and aperture and enhancing stomatal sensitivity to ABA rather than increasing leaf [ABA].

View Article: PubMed Central - PubMed

Affiliation: Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa Porto, Portugal.

ABSTRACT
High relative air humidity (RH ≥ 85%) during growth leads to stomata malfunctioning, resulting in water stress when plants are transferred to conditions of high evaporative demand. In this study, we hypothesized that an elevated air movement (MOV) 24 h per day, during the whole period of leaf development would increase abscisic acid concentration ([ABA]) enhancing stomatal functioning. Pot rose 'Toril' was grown at moderate (61%) or high (92%) RH combined with a continuous low (0.08 m s(-1)) or high (0.92 m s(-1)) MOV. High MOV reduced stomatal pore length and aperture in plants developed at high RH. Moreover, stomatal function improved when high MOV-treated plants were subjected to leaflet desiccation and ABA feeding. Endogenous concentration of ABA and its metabolites in the leaves was reduced by 35% in high RH, but contrary to our hypothesis this concentration was not significantly affected by high MOV. Interestingly, in detached leaflets grown at high RH, high MOV increased stomatal sensitivity to ABA since the amount of exogenous ABA required to decrease the transpiration rate was significantly reduced. This is the first study to show that high MOV increases stomatal functionality in leaves developed at high RH by reducing the stomatal pore length and aperture and enhancing stomatal sensitivity to ABA rather than increasing leaf [ABA].

No MeSH data available.


Related in: MedlinePlus