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The Expression of Notch/Notch Ligand, IL-35, IL-17, and Th17/Treg in Preeclampsia.

Cao W, Wang X, Chen T, Zhu H, Xu W, Zhao S, Cheng X, Xia L - Dis. Markers (2015)

Bottom Line: It was observed that the expressions of Foxp3, EBI3, and P35 in PE patients were lower compared with normal pregnancy, whereas the RORγt expression was significantly increased.The results also demonstrated that PE patients exhibited decreased levels of Treg-related cytokine IL-35, whereas IL-17 was significantly increased.Our results indicated that maternal immunological changes may reverse maternal tolerance in PE, and this phenomenon may due to the Th17/Treg imbalance affected by Notch/Notch ligand.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics, Maternity and Child Health Hospital of Zhenjiang, No. 20 Zhengdong Road, Zhenjiang, Jiangsu 212001, China.

ABSTRACT

Unlabelled: The aim of this study was to examine the interaction of Notch/Notch ligand with Th17/Treg, cytokines IL-35 and IL-17 in cases of preeclampsia (PE).

Methods: Peripheral blood was obtained from 42 PE patients and 22 health pregnant women. The mRNA expressions of Notch/Notch ligand, Treg transcription factor FoxP3 and Th17 transcription factor RORγt, EBI3 and P35 (IL-35 two subunits), and IL-17 were determined by qPCR. The serum levels of IL-17 and IL-35 were measured by ELISA.

Results: It was observed that the expressions of Foxp3, EBI3, and P35 in PE patients were lower compared with normal pregnancy, whereas the RORγt expression was significantly increased. The results also demonstrated that PE patients exhibited decreased levels of Treg-related cytokine IL-35, whereas IL-17 was significantly increased. PE patients expressed higher levels of Notch receptor (1-4) and Notch ligand of DLL4, whereas Notch ligand of Jagged-1, -2 was much lower. Furthermore, the levels of FoxP3 T cells correlated positively with Jagged-2. In addition, there were positive correlations between the mRNA level of IL-17 and DLL4.

Conclusion: Our results indicated that maternal immunological changes may reverse maternal tolerance in PE, and this phenomenon may due to the Th17/Treg imbalance affected by Notch/Notch ligand.

No MeSH data available.


Related in: MedlinePlus

Expression of transcription factors in PBMC of PE patients and normal pregnant women. mRNA expression in PBMC from PE patients was measured by PCR. The result was normalized relative to beta actin and compared to expression of these mRNA from 22 normal pregnant women. (a) The relative expression of Foxp3 was compared among three groups; (b) the relative expression of RORγt was compared among three groups.
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fig1: Expression of transcription factors in PBMC of PE patients and normal pregnant women. mRNA expression in PBMC from PE patients was measured by PCR. The result was normalized relative to beta actin and compared to expression of these mRNA from 22 normal pregnant women. (a) The relative expression of Foxp3 was compared among three groups; (b) the relative expression of RORγt was compared among three groups.

Mentions: In this study, we determined the specific transcription factor of Th17 and Treg cells in three groups by real-time PCR. As shown in Figure 1, decreased mRNA expression of Treg specific transcription factor Foxp3 was observed in server PE patients compared with normal pregnant women (P < 0.001); as for Th17 specific transcription factor, RORγt mRNA expression was significantly higher in mild PE and severe PE patients than those normal controls (both P < 0.05).


The Expression of Notch/Notch Ligand, IL-35, IL-17, and Th17/Treg in Preeclampsia.

Cao W, Wang X, Chen T, Zhu H, Xu W, Zhao S, Cheng X, Xia L - Dis. Markers (2015)

Expression of transcription factors in PBMC of PE patients and normal pregnant women. mRNA expression in PBMC from PE patients was measured by PCR. The result was normalized relative to beta actin and compared to expression of these mRNA from 22 normal pregnant women. (a) The relative expression of Foxp3 was compared among three groups; (b) the relative expression of RORγt was compared among three groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4446509&req=5

fig1: Expression of transcription factors in PBMC of PE patients and normal pregnant women. mRNA expression in PBMC from PE patients was measured by PCR. The result was normalized relative to beta actin and compared to expression of these mRNA from 22 normal pregnant women. (a) The relative expression of Foxp3 was compared among three groups; (b) the relative expression of RORγt was compared among three groups.
Mentions: In this study, we determined the specific transcription factor of Th17 and Treg cells in three groups by real-time PCR. As shown in Figure 1, decreased mRNA expression of Treg specific transcription factor Foxp3 was observed in server PE patients compared with normal pregnant women (P < 0.001); as for Th17 specific transcription factor, RORγt mRNA expression was significantly higher in mild PE and severe PE patients than those normal controls (both P < 0.05).

Bottom Line: It was observed that the expressions of Foxp3, EBI3, and P35 in PE patients were lower compared with normal pregnancy, whereas the RORγt expression was significantly increased.The results also demonstrated that PE patients exhibited decreased levels of Treg-related cytokine IL-35, whereas IL-17 was significantly increased.Our results indicated that maternal immunological changes may reverse maternal tolerance in PE, and this phenomenon may due to the Th17/Treg imbalance affected by Notch/Notch ligand.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics, Maternity and Child Health Hospital of Zhenjiang, No. 20 Zhengdong Road, Zhenjiang, Jiangsu 212001, China.

ABSTRACT

Unlabelled: The aim of this study was to examine the interaction of Notch/Notch ligand with Th17/Treg, cytokines IL-35 and IL-17 in cases of preeclampsia (PE).

Methods: Peripheral blood was obtained from 42 PE patients and 22 health pregnant women. The mRNA expressions of Notch/Notch ligand, Treg transcription factor FoxP3 and Th17 transcription factor RORγt, EBI3 and P35 (IL-35 two subunits), and IL-17 were determined by qPCR. The serum levels of IL-17 and IL-35 were measured by ELISA.

Results: It was observed that the expressions of Foxp3, EBI3, and P35 in PE patients were lower compared with normal pregnancy, whereas the RORγt expression was significantly increased. The results also demonstrated that PE patients exhibited decreased levels of Treg-related cytokine IL-35, whereas IL-17 was significantly increased. PE patients expressed higher levels of Notch receptor (1-4) and Notch ligand of DLL4, whereas Notch ligand of Jagged-1, -2 was much lower. Furthermore, the levels of FoxP3 T cells correlated positively with Jagged-2. In addition, there were positive correlations between the mRNA level of IL-17 and DLL4.

Conclusion: Our results indicated that maternal immunological changes may reverse maternal tolerance in PE, and this phenomenon may due to the Th17/Treg imbalance affected by Notch/Notch ligand.

No MeSH data available.


Related in: MedlinePlus