A novel sigma factor reveals a unique regulon controlling cell-specific recombination in Mycoplasma genitalium.
Bottom Line: Herein, we found that MG428 activates transcription of recA, ruvA and ruvB as well as several genes with unknown function.Additionally, MG428 was found to interact with the RNAP core enzyme, reinforcing the predicted role of this protein as an alternative sigma factor.Finally, our results indicate that MG428 contributes to the generation of genetic diversity in this model organism.
Affiliation: Institut de Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular. Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.Show MeSH
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Mentions: The existence of transcriptional changes between the WT strain and the different MG_428 mutants described above was analyzed by qRT-PCR (Figure 3). As expected, no MG_428 transcript could be detected in the ΔMG_428 mutant. However, in agreement with the western blot analysis, we found increased levels of MG_428 transcript (∼20-fold) in the complemented mutants as compared to the WT strain. No transcriptional changes of the MG_427 gene were observed in any of the strains tested, suggesting that MG428 overexpression has no effect on MG_427 transcript levels.
Affiliation: Institut de Biotecnologia i Biomedicina and Departament de Bioquímica i Biologia Molecular. Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.