Splice junctions are constrained by protein disorder.
Bottom Line: Thus we infer that it is the positions of splice junctions in the gene that must be under constraint by the local protein environment.Examining exonic splicing enhancers found near the splice junction in the gene, reveals that these (short DNA motifs) are more prevalent in exons that encode disordered protein regions than exons encoding structured regions.Thus we also conclude that local protein features constrain efficient splicing more in structure than in disorder.
Affiliation: Department of Computer Science, University of Bristol, Bristol, BS8 1UB, UK firstname.lastname@example.org.Show MeSH
Mentions: We compared the amino acids that are preferred or avoided near splice junctions between six different taxonomic groups and found that most trends are consistent, with some notable exceptions. Figure 6 shows some illustrative examples of the distribution of correlation coefficients for each taxonomic grouping; results for all amino acids and each exon class can be found online at http://bioinformatics.bris.ac.uk/people/ben_smithers/splicing.
Affiliation: Department of Computer Science, University of Bristol, Bristol, BS8 1UB, UK email@example.com.