H3K4 monomethylation dictates nucleosome dynamics and chromatin remodeling at stress-responsive genes.
Bottom Line: Hog1 targets the RSC chromatin remodeling complex to stress-responsive genes and rsc deficient cells display reduced induction of gene expression.Monomethylation of H3K4 is specifically inhibiting RSC-independent chromatin remodeling and thus, it prevents osmostress-induced gene expression.Our findings point to a novel role for H3K4 monomethylation in dictating the specificity of chromatin remodeling, adding an extra layer of regulation to the transcriptional stress response.
Affiliation: Cell signaling unit, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra (UPF), Parc de Recerca Biomèdica de Barcelona (PRBB), Barcelona, Spain.Show MeSH
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Mentions: To analyze the role of histone methylation in nucleosome remodeling following osmostress, we then analyzed the nucleosome positioning at the STL1 locus by Micrococcal Nuclease (MNase) digestion of chromatin before and after stress in wild type, rsc9ts, set1Δ and rsc9tsset1Δ strains. As described, RSC action affects nucleosome position in basal conditions (37,38). We found that, as expected, inactivation of Rsc9 prevented the dramatic changes in chromatin structure observed upon stress in the wild type strain. Nevertheless, deletion of SET1 in the rsc9ts background partially restored such chromatin remodeling (Figure 2). Together, our data suggest that in cells deficient for the RSC complex, methylation by Set1 prevents chromatin reorganization upon stress.
Affiliation: Cell signaling unit, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra (UPF), Parc de Recerca Biomèdica de Barcelona (PRBB), Barcelona, Spain.