Limits...
A Taxonomic Revision of the Wallemia sebi Species Complex.

Jančič S, Nguyen HD, Frisvad JC, Zalar P, Schroers HJ, Seifert KA, Gunde-Cimerman N - PLoS ONE (2015)

Bottom Line: Canadensis, and W. tropicalis.The species differ in their conidial size, xerotolerance, halotolerance, chaotolerance, growth temperature regimes, extracellular enzyme activity profiles, and secondary metabolite patterns.A key to all currently accepted Wallemia species is provided that allow their identification on the basis of physiological, micromorphological and culture characters.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.

ABSTRACT
Wallemia sebi is a xerophilic food- and air-borne fungus. The name has been used for strains that prevail in cold, temperate and tropical climates. In this study, multi-locus phylogenetic analyses, using the internal transcribed spacer (ITS) regions, DNA replication licensing factor (MCM7), pre-rRNA processing protein (TSR1), RNA polymerase II largest subunit (RPB1), RNA polymerase II second largest subunit (RPB2) and a new marker 3´-phosphoadenosine-5´-phosphatase (HAL2), confirmed the previous hypothesis that W. sebi presents a complex of at least four species. Here, we confirm and apply the phylogenetic analyses based species hypotheses from a companion study to guide phenotypic assessment of W. sebi like strains from a wide range of substrates, climates and continents allowed the recognition of W. sebi sensu stricto and three new species described as W. mellicola, W. Canadensis, and W. tropicalis. The species differ in their conidial size, xerotolerance, halotolerance, chaotolerance, growth temperature regimes, extracellular enzyme activity profiles, and secondary metabolite patterns. A key to all currently accepted Wallemia species is provided that allow their identification on the basis of physiological, micromorphological and culture characters.

No MeSH data available.


Related in: MedlinePlus

Midpoint rooted majority rule consensus tree of Bayesian MCMC sampling inferred from the HAL2 sequences.Bayesian posterior probabilities are displayed at the nodes of the tree. Red T, ex-type strains; red NT, ex-neotype strain; bold, strains included in physiological and morphological studies, and for the determination of extracellular enzyme activities; underlined, strains included in studies of secondary metabolites.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4446336&req=5

pone.0125933.g002: Midpoint rooted majority rule consensus tree of Bayesian MCMC sampling inferred from the HAL2 sequences.Bayesian posterior probabilities are displayed at the nodes of the tree. Red T, ex-type strains; red NT, ex-neotype strain; bold, strains included in physiological and morphological studies, and for the determination of extracellular enzyme activities; underlined, strains included in studies of secondary metabolites.

Mentions: The ITS phylogenetic analysis divided the WSSC into two subgroups, with only the subgroup including the ex-neotype strain CBS 818.96 strongly supported. Most strains of the WSSC formed an un-resolved and weakly supported cluster (S1 Fig). Analyses of the rpb2, rpb1, MCM7, and tsr1 sequences resolved the WSSC into four groups, referred to as clades 1–4. The tree topologies based on rpb1, MCM7, and tsr1 (S1 File) were similar to the topology of the combined data set (Fig 1), suggesting a sister-group relationship of clades 1 and 2. Clades 1–4 also received high support in analyses of the hal2 sequences (Fig 2), with a supported sister group relationship for clades 2 and 4. Comparison of pair-wise distance (p-distance), alignment length, and parsimony informative characters obtained from HAL2 sequences are provided in S2 Fig and S3 Table. Further details of the phylogenetic analyses, determination of genetic variability of sampled loci and barcode gap analyses are described in the companion study [27].


A Taxonomic Revision of the Wallemia sebi Species Complex.

Jančič S, Nguyen HD, Frisvad JC, Zalar P, Schroers HJ, Seifert KA, Gunde-Cimerman N - PLoS ONE (2015)

Midpoint rooted majority rule consensus tree of Bayesian MCMC sampling inferred from the HAL2 sequences.Bayesian posterior probabilities are displayed at the nodes of the tree. Red T, ex-type strains; red NT, ex-neotype strain; bold, strains included in physiological and morphological studies, and for the determination of extracellular enzyme activities; underlined, strains included in studies of secondary metabolites.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4446336&req=5

pone.0125933.g002: Midpoint rooted majority rule consensus tree of Bayesian MCMC sampling inferred from the HAL2 sequences.Bayesian posterior probabilities are displayed at the nodes of the tree. Red T, ex-type strains; red NT, ex-neotype strain; bold, strains included in physiological and morphological studies, and for the determination of extracellular enzyme activities; underlined, strains included in studies of secondary metabolites.
Mentions: The ITS phylogenetic analysis divided the WSSC into two subgroups, with only the subgroup including the ex-neotype strain CBS 818.96 strongly supported. Most strains of the WSSC formed an un-resolved and weakly supported cluster (S1 Fig). Analyses of the rpb2, rpb1, MCM7, and tsr1 sequences resolved the WSSC into four groups, referred to as clades 1–4. The tree topologies based on rpb1, MCM7, and tsr1 (S1 File) were similar to the topology of the combined data set (Fig 1), suggesting a sister-group relationship of clades 1 and 2. Clades 1–4 also received high support in analyses of the hal2 sequences (Fig 2), with a supported sister group relationship for clades 2 and 4. Comparison of pair-wise distance (p-distance), alignment length, and parsimony informative characters obtained from HAL2 sequences are provided in S2 Fig and S3 Table. Further details of the phylogenetic analyses, determination of genetic variability of sampled loci and barcode gap analyses are described in the companion study [27].

Bottom Line: Canadensis, and W. tropicalis.The species differ in their conidial size, xerotolerance, halotolerance, chaotolerance, growth temperature regimes, extracellular enzyme activity profiles, and secondary metabolite patterns.A key to all currently accepted Wallemia species is provided that allow their identification on the basis of physiological, micromorphological and culture characters.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.

ABSTRACT
Wallemia sebi is a xerophilic food- and air-borne fungus. The name has been used for strains that prevail in cold, temperate and tropical climates. In this study, multi-locus phylogenetic analyses, using the internal transcribed spacer (ITS) regions, DNA replication licensing factor (MCM7), pre-rRNA processing protein (TSR1), RNA polymerase II largest subunit (RPB1), RNA polymerase II second largest subunit (RPB2) and a new marker 3´-phosphoadenosine-5´-phosphatase (HAL2), confirmed the previous hypothesis that W. sebi presents a complex of at least four species. Here, we confirm and apply the phylogenetic analyses based species hypotheses from a companion study to guide phenotypic assessment of W. sebi like strains from a wide range of substrates, climates and continents allowed the recognition of W. sebi sensu stricto and three new species described as W. mellicola, W. Canadensis, and W. tropicalis. The species differ in their conidial size, xerotolerance, halotolerance, chaotolerance, growth temperature regimes, extracellular enzyme activity profiles, and secondary metabolite patterns. A key to all currently accepted Wallemia species is provided that allow their identification on the basis of physiological, micromorphological and culture characters.

No MeSH data available.


Related in: MedlinePlus