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The evolution of collagen fiber orientation in engineered cardiovascular tissues visualized by diffusion tensor imaging.

Ghazanfari S, Driessen-Mol A, Strijkers GJ, Baaijens FP, Bouten CV - PLoS ONE (2015)

Bottom Line: Collagen fibers in the high aspect ratio samples were mostly aligned in the constrained direction, while the collagen fibers in low aspect ratio strips were mainly oriented in the oblique direction.DTI captured the collagen orientation differences between low and high aspect ratio samples and with time.Therefore, it can be used as a fast, non-destructive and reliable tool to study the evolution of the collagen orientation in TE constructs.

View Article: PubMed Central - PubMed

Affiliation: Soft Tissue Biomechanics and Engineering, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, the Netherlands.

ABSTRACT
The collagen architecture is the major determinant of the function and mechanical behavior of cardiovascular tissues. In order to engineer a functional and load-bearing cardiovascular tissue with a structure that mimics the native tissue to meet in vivo mechanical demands, a complete understanding of the collagen orientation mechanism is required. Several methods have been used to visualize collagen architecture in tissue-engineered (TE) constructs, but they either have a limited imaging depth or have a complicated set up. In this study, Diffusion Tensor Imaging (DTI) is explored as a fast and reliable method to visualize collagen arrangement, and Confocal Laser Scanning Microscopy (CLSM) was used as a validation technique. Uniaxially constrained TE strips were cultured for 2 days, 10 days, 3 and 6 weeks to investigate the evolution of the collagen orientation with time. Moreover, a comparison of the collagen orientation in high and low aspect ratio (length/width) TE constructs was made with both methods. Both methods showed similar fiber orientation in TE constructs. Collagen fibers in the high aspect ratio samples were mostly aligned in the constrained direction, while the collagen fibers in low aspect ratio strips were mainly oriented in the oblique direction. The orientation changed to the oblique direction by extending culture time and could also be visualized. DTI captured the collagen orientation differences between low and high aspect ratio samples and with time. Therefore, it can be used as a fast, non-destructive and reliable tool to study the evolution of the collagen orientation in TE constructs.

No MeSH data available.


Hematoxylin and eosin staining of low (A) and high (C) aspect ratio TE sample after 3 weeks of culture.Both samples were compacted after 3 weeks. The scale bar represents 500 μm.
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pone.0127847.g010: Hematoxylin and eosin staining of low (A) and high (C) aspect ratio TE sample after 3 weeks of culture.Both samples were compacted after 3 weeks. The scale bar represents 500 μm.

Mentions: H&E staining of slices representing the cross-sectional area of the strips shows the rectangular shape of a typical representative example of TE strips after 2 days of culture, which became more round due to tissue compaction after extending the culture time to 6 weeks (Fig 9). This observation was in agreement with the observed reduction in width of the samples when visualized from top view using the imaging modalities. Fig 10 shows the H&E staining of high and low aspect ratio samples. Both samples demonstrated compaction after 3 weeks of culture.


The evolution of collagen fiber orientation in engineered cardiovascular tissues visualized by diffusion tensor imaging.

Ghazanfari S, Driessen-Mol A, Strijkers GJ, Baaijens FP, Bouten CV - PLoS ONE (2015)

Hematoxylin and eosin staining of low (A) and high (C) aspect ratio TE sample after 3 weeks of culture.Both samples were compacted after 3 weeks. The scale bar represents 500 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4446330&req=5

pone.0127847.g010: Hematoxylin and eosin staining of low (A) and high (C) aspect ratio TE sample after 3 weeks of culture.Both samples were compacted after 3 weeks. The scale bar represents 500 μm.
Mentions: H&E staining of slices representing the cross-sectional area of the strips shows the rectangular shape of a typical representative example of TE strips after 2 days of culture, which became more round due to tissue compaction after extending the culture time to 6 weeks (Fig 9). This observation was in agreement with the observed reduction in width of the samples when visualized from top view using the imaging modalities. Fig 10 shows the H&E staining of high and low aspect ratio samples. Both samples demonstrated compaction after 3 weeks of culture.

Bottom Line: Collagen fibers in the high aspect ratio samples were mostly aligned in the constrained direction, while the collagen fibers in low aspect ratio strips were mainly oriented in the oblique direction.DTI captured the collagen orientation differences between low and high aspect ratio samples and with time.Therefore, it can be used as a fast, non-destructive and reliable tool to study the evolution of the collagen orientation in TE constructs.

View Article: PubMed Central - PubMed

Affiliation: Soft Tissue Biomechanics and Engineering, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, the Netherlands.

ABSTRACT
The collagen architecture is the major determinant of the function and mechanical behavior of cardiovascular tissues. In order to engineer a functional and load-bearing cardiovascular tissue with a structure that mimics the native tissue to meet in vivo mechanical demands, a complete understanding of the collagen orientation mechanism is required. Several methods have been used to visualize collagen architecture in tissue-engineered (TE) constructs, but they either have a limited imaging depth or have a complicated set up. In this study, Diffusion Tensor Imaging (DTI) is explored as a fast and reliable method to visualize collagen arrangement, and Confocal Laser Scanning Microscopy (CLSM) was used as a validation technique. Uniaxially constrained TE strips were cultured for 2 days, 10 days, 3 and 6 weeks to investigate the evolution of the collagen orientation with time. Moreover, a comparison of the collagen orientation in high and low aspect ratio (length/width) TE constructs was made with both methods. Both methods showed similar fiber orientation in TE constructs. Collagen fibers in the high aspect ratio samples were mostly aligned in the constrained direction, while the collagen fibers in low aspect ratio strips were mainly oriented in the oblique direction. The orientation changed to the oblique direction by extending culture time and could also be visualized. DTI captured the collagen orientation differences between low and high aspect ratio samples and with time. Therefore, it can be used as a fast, non-destructive and reliable tool to study the evolution of the collagen orientation in TE constructs.

No MeSH data available.