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Ancient DNA analysis of the oldest canid species from the Siberian Arctic and genetic contribution to the domestic dog.

Lee EJ, Merriwether DA, Kasparov AK, Nikolskiy PA, Sotnikova MV, Pavlova EY, Pitulko VV - PLoS ONE (2015)

Bottom Line: In order to examine the genetic affinities of ancient Siberian canids species to the domestic dog and modern wolves, we obtained mitochondrial DNA control region sequences and compared them to published ancient and modern canid sequences.The older canid specimens illustrate affinities with pre-domestic dog/wolf lineages while others appear in the major phylogenetic clades of domestic dogs.Our results suggest a European origin of domestic dog may not be conclusive and illustrates an emerging complexity of genetic contribution of regional wolf breeds to the modern Canis gene pool.

View Article: PubMed Central - PubMed

Affiliation: Department of Anthropology, Binghamton University, Binghamton, NY, United States of America; Department of Sociology, Anthropology, and Social Work, Texas Tech University, Lubbock, TX, United States of America.

ABSTRACT
Modern Arctic Siberia provides a wealth of resources for archaeological, geological, and paleontological research to investigate the population dynamics of faunal communities from the Pleistocene, particularly as the faunal material coming from permafrost has proven suitable for genetic studies. In order to examine the history of the Canid species in the Siberian Arctic, we carried out genetic analysis of fourteen canid remains from various sites, including the well-documented Upper Paleolithic Yana RHS and Early Holocene Zhokhov Island sites. Estimated age of samples range from as recent as 1,700 years before present (YBP) to at least 360,000 YBP for the remains of the extinct wolf, Canis cf. variabilis. In order to examine the genetic affinities of ancient Siberian canids species to the domestic dog and modern wolves, we obtained mitochondrial DNA control region sequences and compared them to published ancient and modern canid sequences. The older canid specimens illustrate affinities with pre-domestic dog/wolf lineages while others appear in the major phylogenetic clades of domestic dogs. Our results suggest a European origin of domestic dog may not be conclusive and illustrates an emerging complexity of genetic contribution of regional wolf breeds to the modern Canis gene pool.

No MeSH data available.


Location of the sites studied.Corresponding numbers and information are provided in Table 1.
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pone.0125759.g001: Location of the sites studied.Corresponding numbers and information are provided in Table 1.

Mentions: We examined thirteen prehistoric canid remains and one contemporary wolf sample from the Siberian Arctic: Ulakhan-Sullar, Duvanny Yar, Yana RHS, Zhokhov Island, and Aachim Lighthouse (Fig 1 and Table 1). The oldest specimens come from the exposures of Quaternary deposits (locality #1, Canis cf. variabilis) and from Duvanny Yar exposure (locality #2), Details of each site are provided in the next section. Mitochondrial DNA (mtDNA) analysis of the canid specimens was carried out to infer the phylogenetic relationships of these ancient canids with modern canid species, with particular focus on the Canis cf. variabilis specimen from Ulakhan-Sullar that may provide clues to the origin of domestic dogs.


Ancient DNA analysis of the oldest canid species from the Siberian Arctic and genetic contribution to the domestic dog.

Lee EJ, Merriwether DA, Kasparov AK, Nikolskiy PA, Sotnikova MV, Pavlova EY, Pitulko VV - PLoS ONE (2015)

Location of the sites studied.Corresponding numbers and information are provided in Table 1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4446326&req=5

pone.0125759.g001: Location of the sites studied.Corresponding numbers and information are provided in Table 1.
Mentions: We examined thirteen prehistoric canid remains and one contemporary wolf sample from the Siberian Arctic: Ulakhan-Sullar, Duvanny Yar, Yana RHS, Zhokhov Island, and Aachim Lighthouse (Fig 1 and Table 1). The oldest specimens come from the exposures of Quaternary deposits (locality #1, Canis cf. variabilis) and from Duvanny Yar exposure (locality #2), Details of each site are provided in the next section. Mitochondrial DNA (mtDNA) analysis of the canid specimens was carried out to infer the phylogenetic relationships of these ancient canids with modern canid species, with particular focus on the Canis cf. variabilis specimen from Ulakhan-Sullar that may provide clues to the origin of domestic dogs.

Bottom Line: In order to examine the genetic affinities of ancient Siberian canids species to the domestic dog and modern wolves, we obtained mitochondrial DNA control region sequences and compared them to published ancient and modern canid sequences.The older canid specimens illustrate affinities with pre-domestic dog/wolf lineages while others appear in the major phylogenetic clades of domestic dogs.Our results suggest a European origin of domestic dog may not be conclusive and illustrates an emerging complexity of genetic contribution of regional wolf breeds to the modern Canis gene pool.

View Article: PubMed Central - PubMed

Affiliation: Department of Anthropology, Binghamton University, Binghamton, NY, United States of America; Department of Sociology, Anthropology, and Social Work, Texas Tech University, Lubbock, TX, United States of America.

ABSTRACT
Modern Arctic Siberia provides a wealth of resources for archaeological, geological, and paleontological research to investigate the population dynamics of faunal communities from the Pleistocene, particularly as the faunal material coming from permafrost has proven suitable for genetic studies. In order to examine the history of the Canid species in the Siberian Arctic, we carried out genetic analysis of fourteen canid remains from various sites, including the well-documented Upper Paleolithic Yana RHS and Early Holocene Zhokhov Island sites. Estimated age of samples range from as recent as 1,700 years before present (YBP) to at least 360,000 YBP for the remains of the extinct wolf, Canis cf. variabilis. In order to examine the genetic affinities of ancient Siberian canids species to the domestic dog and modern wolves, we obtained mitochondrial DNA control region sequences and compared them to published ancient and modern canid sequences. The older canid specimens illustrate affinities with pre-domestic dog/wolf lineages while others appear in the major phylogenetic clades of domestic dogs. Our results suggest a European origin of domestic dog may not be conclusive and illustrates an emerging complexity of genetic contribution of regional wolf breeds to the modern Canis gene pool.

No MeSH data available.