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Laser-stimulated fluorescence in paleontology.

Kaye TG, Falk AR, Pittman M, Sereno PC, Martin LD, Burnham DA, Gong E, Xu X, Wang Y - PLoS ONE (2015)

Bottom Line: A laser's ability to concentrate very high flux rates both at the macroscopic and microscopic levels results in specimens fluorescing in ways a standard UV bulb cannot induce.The recent cost reductions in medium-power short wavelength lasers and use of standard photographic filters has now made this technique widely accessible to researchers.This represents a highly cost-effective way to address paleontology's preparatory bottleneck.

View Article: PubMed Central - PubMed

Affiliation: Burke Museum of Natural History and Culture, Seattle, Washington, United States of America.

ABSTRACT
Fluorescence using ultraviolet (UV) light has seen increased use as a tool in paleontology over the last decade. Laser-stimulated fluorescence (LSF) is a next generation technique that is emerging as a way to fluoresce paleontological specimens that remain dark under typical UV. A laser's ability to concentrate very high flux rates both at the macroscopic and microscopic levels results in specimens fluorescing in ways a standard UV bulb cannot induce. Presented here are five paleontological case histories that illustrate the technique across a broad range of specimens and scales. Novel uses such as back-lighting opaque specimens to reveal detail and detection of specimens completely obscured by matrix are highlighted in these examples. The recent cost reductions in medium-power short wavelength lasers and use of standard photographic filters has now made this technique widely accessible to researchers. This technology has the potential to automate multiple aspects of paleontology, including preparation and sorting of microfossils. This represents a highly cost-effective way to address paleontology's preparatory bottleneck.

No MeSH data available.


Is the skull of Microraptor IVPP V13320 a composite?A, the skull of IVPP V13320 under white light conditions shows subtle color differences in the bone across a break in the slab—darker bone proximally and lighter bone distally. B, Under laser light stimulation, the bone fluoresces with the same color pattern observed under white light conditions (see A) indicating that the color differences relate to differences in fossil mineralogy. The latter indicates that the skull is a composite specimen, but it is also possible—but less likely—that the pattern observed reflects variable depositional and taphonomic conditions. Scale bar 1 cm. S2 Fig is a labelled version of this figure (see Supporting Information).
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pone.0125923.g013: Is the skull of Microraptor IVPP V13320 a composite?A, the skull of IVPP V13320 under white light conditions shows subtle color differences in the bone across a break in the slab—darker bone proximally and lighter bone distally. B, Under laser light stimulation, the bone fluoresces with the same color pattern observed under white light conditions (see A) indicating that the color differences relate to differences in fossil mineralogy. The latter indicates that the skull is a composite specimen, but it is also possible—but less likely—that the pattern observed reflects variable depositional and taphonomic conditions. Scale bar 1 cm. S2 Fig is a labelled version of this figure (see Supporting Information).

Mentions: The skull of a Microraptor specimen (IVPP V13320 [21]) was first examined under white light conditions. Although there are subtle color differences in the fossil, nothing unusual stands out on first inspection (Fig 13A). Subsequent imaging using LSF reveals dramatic differences in fluorescence between the proximal and distal portions of the skull prompting further investigation (Fig 13B).


Laser-stimulated fluorescence in paleontology.

Kaye TG, Falk AR, Pittman M, Sereno PC, Martin LD, Burnham DA, Gong E, Xu X, Wang Y - PLoS ONE (2015)

Is the skull of Microraptor IVPP V13320 a composite?A, the skull of IVPP V13320 under white light conditions shows subtle color differences in the bone across a break in the slab—darker bone proximally and lighter bone distally. B, Under laser light stimulation, the bone fluoresces with the same color pattern observed under white light conditions (see A) indicating that the color differences relate to differences in fossil mineralogy. The latter indicates that the skull is a composite specimen, but it is also possible—but less likely—that the pattern observed reflects variable depositional and taphonomic conditions. Scale bar 1 cm. S2 Fig is a labelled version of this figure (see Supporting Information).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4446324&req=5

pone.0125923.g013: Is the skull of Microraptor IVPP V13320 a composite?A, the skull of IVPP V13320 under white light conditions shows subtle color differences in the bone across a break in the slab—darker bone proximally and lighter bone distally. B, Under laser light stimulation, the bone fluoresces with the same color pattern observed under white light conditions (see A) indicating that the color differences relate to differences in fossil mineralogy. The latter indicates that the skull is a composite specimen, but it is also possible—but less likely—that the pattern observed reflects variable depositional and taphonomic conditions. Scale bar 1 cm. S2 Fig is a labelled version of this figure (see Supporting Information).
Mentions: The skull of a Microraptor specimen (IVPP V13320 [21]) was first examined under white light conditions. Although there are subtle color differences in the fossil, nothing unusual stands out on first inspection (Fig 13A). Subsequent imaging using LSF reveals dramatic differences in fluorescence between the proximal and distal portions of the skull prompting further investigation (Fig 13B).

Bottom Line: A laser's ability to concentrate very high flux rates both at the macroscopic and microscopic levels results in specimens fluorescing in ways a standard UV bulb cannot induce.The recent cost reductions in medium-power short wavelength lasers and use of standard photographic filters has now made this technique widely accessible to researchers.This represents a highly cost-effective way to address paleontology's preparatory bottleneck.

View Article: PubMed Central - PubMed

Affiliation: Burke Museum of Natural History and Culture, Seattle, Washington, United States of America.

ABSTRACT
Fluorescence using ultraviolet (UV) light has seen increased use as a tool in paleontology over the last decade. Laser-stimulated fluorescence (LSF) is a next generation technique that is emerging as a way to fluoresce paleontological specimens that remain dark under typical UV. A laser's ability to concentrate very high flux rates both at the macroscopic and microscopic levels results in specimens fluorescing in ways a standard UV bulb cannot induce. Presented here are five paleontological case histories that illustrate the technique across a broad range of specimens and scales. Novel uses such as back-lighting opaque specimens to reveal detail and detection of specimens completely obscured by matrix are highlighted in these examples. The recent cost reductions in medium-power short wavelength lasers and use of standard photographic filters has now made this technique widely accessible to researchers. This technology has the potential to automate multiple aspects of paleontology, including preparation and sorting of microfossils. This represents a highly cost-effective way to address paleontology's preparatory bottleneck.

No MeSH data available.