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The Effect of Pre-Condition Cerebella Fastigial Nucleus Electrical Stimulation within and beyond the Time Window of Thrombolytic on Ischemic Stroke in the Rats.

Tang W, Dong W, Xie P, Cheng P, Bai S, Ren Y, Wang G, Chen X, Cui C, Zhuang Y, Huang W - PLoS ONE (2015)

Bottom Line: To investigate the effect of neurogenic neuroprotection conferred by cerebellar fastigial nucleus stimulation (FNS) and the role of PPARγ-mediated inflammation in a rat model of cerebral ischemia reperfusion.After a continuous 1 hour fastigial nucleus electric stimulation, the male Sprague Dawley (SD) rats were given middle cerebral artery occlusion (MCAO) for 1, 3, 6, 9, 12 and 15 hours undergoing reperfusion with intravenous recombinant tissue plasminogen activator (rt-PA), while the control group received without FNS.The brain tissue in ischemic penumbra was determined the myeloperoxidase (MPO) activity by a spectrophotometer and expression of PPARγ was measured by Rt-PCR and Western blotting.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT

Objective: To investigate the effect of neurogenic neuroprotection conferred by cerebellar fastigial nucleus stimulation (FNS) and the role of PPARγ-mediated inflammation in a rat model of cerebral ischemia reperfusion.

Methods: After a continuous 1 hour fastigial nucleus electric stimulation, the male Sprague Dawley (SD) rats were given middle cerebral artery occlusion (MCAO) for 1, 3, 6, 9, 12 and 15 hours undergoing reperfusion with intravenous recombinant tissue plasminogen activator (rt-PA), while the control group received without FNS. After 72 h of reperfusion, the neurological deficits, infarct volume and brain edema were evaluated. The brain tissue in ischemic penumbra was determined the myeloperoxidase (MPO) activity by a spectrophotometer and expression of PPARγ was measured by Rt-PCR and Western blotting.

Results: Our findings showed that FNS group had significantly reduced infarct volume and brain edema, and improved neurological deficits compared with the control group, especially in 6 h and 9 h reperfusion subgroups (p<0.05). The expression levels of PPARγ increased gradually and the peak may be before and after 9 h reperfusion, the 3 h, 6 h, 9 h, 12 h and 15 h reperfusion subgroups were higher than each control group (p<0.05). The MPO activity of 6 h, 12 h and 15 h reperfusion subgroups were higher than each control group (p<0.05).

Conclusions: The neuroprotective effects of FNS have been shown to prolong the therapeutic window in cerebral ischemia/reperfusion, which might be related to the PPARγ mediated-inflammation in penumbral region.

No MeSH data available.


Related in: MedlinePlus

Brain water content of two groups.(A) The percentage of ischemic lesion area was represented as the ratio of the infarction area to the whole slice area. (B)Water content was calculated by (wet weight −dry weight) /wet weight × 100%. Results are expressed as the mean ± SD. *p < 0.05, vs the control group, n = 5.
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pone.0128447.g007: Brain water content of two groups.(A) The percentage of ischemic lesion area was represented as the ratio of the infarction area to the whole slice area. (B)Water content was calculated by (wet weight −dry weight) /wet weight × 100%. Results are expressed as the mean ± SD. *p < 0.05, vs the control group, n = 5.

Mentions: Infarct tissues were visualized as an area of unstained part in the ischemia/reperfusion group, in contrast to the viable tissue, which stained red. As shown in Figs 6 and 7 and S3 File, in the FNS group, the infracted volume and the brain water content were significantly reduced as compared with the control group, especially at 6h and 9h reperfusion (P < 0.05).


The Effect of Pre-Condition Cerebella Fastigial Nucleus Electrical Stimulation within and beyond the Time Window of Thrombolytic on Ischemic Stroke in the Rats.

Tang W, Dong W, Xie P, Cheng P, Bai S, Ren Y, Wang G, Chen X, Cui C, Zhuang Y, Huang W - PLoS ONE (2015)

Brain water content of two groups.(A) The percentage of ischemic lesion area was represented as the ratio of the infarction area to the whole slice area. (B)Water content was calculated by (wet weight −dry weight) /wet weight × 100%. Results are expressed as the mean ± SD. *p < 0.05, vs the control group, n = 5.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4446308&req=5

pone.0128447.g007: Brain water content of two groups.(A) The percentage of ischemic lesion area was represented as the ratio of the infarction area to the whole slice area. (B)Water content was calculated by (wet weight −dry weight) /wet weight × 100%. Results are expressed as the mean ± SD. *p < 0.05, vs the control group, n = 5.
Mentions: Infarct tissues were visualized as an area of unstained part in the ischemia/reperfusion group, in contrast to the viable tissue, which stained red. As shown in Figs 6 and 7 and S3 File, in the FNS group, the infracted volume and the brain water content were significantly reduced as compared with the control group, especially at 6h and 9h reperfusion (P < 0.05).

Bottom Line: To investigate the effect of neurogenic neuroprotection conferred by cerebellar fastigial nucleus stimulation (FNS) and the role of PPARγ-mediated inflammation in a rat model of cerebral ischemia reperfusion.After a continuous 1 hour fastigial nucleus electric stimulation, the male Sprague Dawley (SD) rats were given middle cerebral artery occlusion (MCAO) for 1, 3, 6, 9, 12 and 15 hours undergoing reperfusion with intravenous recombinant tissue plasminogen activator (rt-PA), while the control group received without FNS.The brain tissue in ischemic penumbra was determined the myeloperoxidase (MPO) activity by a spectrophotometer and expression of PPARγ was measured by Rt-PCR and Western blotting.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT

Objective: To investigate the effect of neurogenic neuroprotection conferred by cerebellar fastigial nucleus stimulation (FNS) and the role of PPARγ-mediated inflammation in a rat model of cerebral ischemia reperfusion.

Methods: After a continuous 1 hour fastigial nucleus electric stimulation, the male Sprague Dawley (SD) rats were given middle cerebral artery occlusion (MCAO) for 1, 3, 6, 9, 12 and 15 hours undergoing reperfusion with intravenous recombinant tissue plasminogen activator (rt-PA), while the control group received without FNS. After 72 h of reperfusion, the neurological deficits, infarct volume and brain edema were evaluated. The brain tissue in ischemic penumbra was determined the myeloperoxidase (MPO) activity by a spectrophotometer and expression of PPARγ was measured by Rt-PCR and Western blotting.

Results: Our findings showed that FNS group had significantly reduced infarct volume and brain edema, and improved neurological deficits compared with the control group, especially in 6 h and 9 h reperfusion subgroups (p<0.05). The expression levels of PPARγ increased gradually and the peak may be before and after 9 h reperfusion, the 3 h, 6 h, 9 h, 12 h and 15 h reperfusion subgroups were higher than each control group (p<0.05). The MPO activity of 6 h, 12 h and 15 h reperfusion subgroups were higher than each control group (p<0.05).

Conclusions: The neuroprotective effects of FNS have been shown to prolong the therapeutic window in cerebral ischemia/reperfusion, which might be related to the PPARγ mediated-inflammation in penumbral region.

No MeSH data available.


Related in: MedlinePlus