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The Effect of Pre-Condition Cerebella Fastigial Nucleus Electrical Stimulation within and beyond the Time Window of Thrombolytic on Ischemic Stroke in the Rats.

Tang W, Dong W, Xie P, Cheng P, Bai S, Ren Y, Wang G, Chen X, Cui C, Zhuang Y, Huang W - PLoS ONE (2015)

Bottom Line: To investigate the effect of neurogenic neuroprotection conferred by cerebellar fastigial nucleus stimulation (FNS) and the role of PPARγ-mediated inflammation in a rat model of cerebral ischemia reperfusion.After a continuous 1 hour fastigial nucleus electric stimulation, the male Sprague Dawley (SD) rats were given middle cerebral artery occlusion (MCAO) for 1, 3, 6, 9, 12 and 15 hours undergoing reperfusion with intravenous recombinant tissue plasminogen activator (rt-PA), while the control group received without FNS.The brain tissue in ischemic penumbra was determined the myeloperoxidase (MPO) activity by a spectrophotometer and expression of PPARγ was measured by Rt-PCR and Western blotting.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT

Objective: To investigate the effect of neurogenic neuroprotection conferred by cerebellar fastigial nucleus stimulation (FNS) and the role of PPARγ-mediated inflammation in a rat model of cerebral ischemia reperfusion.

Methods: After a continuous 1 hour fastigial nucleus electric stimulation, the male Sprague Dawley (SD) rats were given middle cerebral artery occlusion (MCAO) for 1, 3, 6, 9, 12 and 15 hours undergoing reperfusion with intravenous recombinant tissue plasminogen activator (rt-PA), while the control group received without FNS. After 72 h of reperfusion, the neurological deficits, infarct volume and brain edema were evaluated. The brain tissue in ischemic penumbra was determined the myeloperoxidase (MPO) activity by a spectrophotometer and expression of PPARγ was measured by Rt-PCR and Western blotting.

Results: Our findings showed that FNS group had significantly reduced infarct volume and brain edema, and improved neurological deficits compared with the control group, especially in 6 h and 9 h reperfusion subgroups (p<0.05). The expression levels of PPARγ increased gradually and the peak may be before and after 9 h reperfusion, the 3 h, 6 h, 9 h, 12 h and 15 h reperfusion subgroups were higher than each control group (p<0.05). The MPO activity of 6 h, 12 h and 15 h reperfusion subgroups were higher than each control group (p<0.05).

Conclusions: The neuroprotective effects of FNS have been shown to prolong the therapeutic window in cerebral ischemia/reperfusion, which might be related to the PPARγ mediated-inflammation in penumbral region.

No MeSH data available.


Related in: MedlinePlus

The mRNA levels of PPARγ in cerebral ischemic penumbra.Results are expressed as the mean ± SD. *p < 0.05, vs the control group, n = 5.
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pone.0128447.g004: The mRNA levels of PPARγ in cerebral ischemic penumbra.Results are expressed as the mean ± SD. *p < 0.05, vs the control group, n = 5.

Mentions: To further analyze FNS-induced anti-inflammatory effects, we also examined the expression of PPARγ using Western-blot (S2 File) and Rt-PCR. The results suggested that PPARγ levels were mainly located in the 6h and 9h reperfusion subgroup (Figs 3 and 4). Compared with control group, the levels of PPARγ was significantly higher in 3h, 6h, 9h, 12h and 15h reperfusion subgroup (P < 0.05). Rt-PCR analysis further confirmed this result. A significantly higher expression of PPARγ were found in the FNS group compared with the control group at 3h、6h、9h、15h reperfusion.


The Effect of Pre-Condition Cerebella Fastigial Nucleus Electrical Stimulation within and beyond the Time Window of Thrombolytic on Ischemic Stroke in the Rats.

Tang W, Dong W, Xie P, Cheng P, Bai S, Ren Y, Wang G, Chen X, Cui C, Zhuang Y, Huang W - PLoS ONE (2015)

The mRNA levels of PPARγ in cerebral ischemic penumbra.Results are expressed as the mean ± SD. *p < 0.05, vs the control group, n = 5.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4446308&req=5

pone.0128447.g004: The mRNA levels of PPARγ in cerebral ischemic penumbra.Results are expressed as the mean ± SD. *p < 0.05, vs the control group, n = 5.
Mentions: To further analyze FNS-induced anti-inflammatory effects, we also examined the expression of PPARγ using Western-blot (S2 File) and Rt-PCR. The results suggested that PPARγ levels were mainly located in the 6h and 9h reperfusion subgroup (Figs 3 and 4). Compared with control group, the levels of PPARγ was significantly higher in 3h, 6h, 9h, 12h and 15h reperfusion subgroup (P < 0.05). Rt-PCR analysis further confirmed this result. A significantly higher expression of PPARγ were found in the FNS group compared with the control group at 3h、6h、9h、15h reperfusion.

Bottom Line: To investigate the effect of neurogenic neuroprotection conferred by cerebellar fastigial nucleus stimulation (FNS) and the role of PPARγ-mediated inflammation in a rat model of cerebral ischemia reperfusion.After a continuous 1 hour fastigial nucleus electric stimulation, the male Sprague Dawley (SD) rats were given middle cerebral artery occlusion (MCAO) for 1, 3, 6, 9, 12 and 15 hours undergoing reperfusion with intravenous recombinant tissue plasminogen activator (rt-PA), while the control group received without FNS.The brain tissue in ischemic penumbra was determined the myeloperoxidase (MPO) activity by a spectrophotometer and expression of PPARγ was measured by Rt-PCR and Western blotting.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing, China.

ABSTRACT

Objective: To investigate the effect of neurogenic neuroprotection conferred by cerebellar fastigial nucleus stimulation (FNS) and the role of PPARγ-mediated inflammation in a rat model of cerebral ischemia reperfusion.

Methods: After a continuous 1 hour fastigial nucleus electric stimulation, the male Sprague Dawley (SD) rats were given middle cerebral artery occlusion (MCAO) for 1, 3, 6, 9, 12 and 15 hours undergoing reperfusion with intravenous recombinant tissue plasminogen activator (rt-PA), while the control group received without FNS. After 72 h of reperfusion, the neurological deficits, infarct volume and brain edema were evaluated. The brain tissue in ischemic penumbra was determined the myeloperoxidase (MPO) activity by a spectrophotometer and expression of PPARγ was measured by Rt-PCR and Western blotting.

Results: Our findings showed that FNS group had significantly reduced infarct volume and brain edema, and improved neurological deficits compared with the control group, especially in 6 h and 9 h reperfusion subgroups (p<0.05). The expression levels of PPARγ increased gradually and the peak may be before and after 9 h reperfusion, the 3 h, 6 h, 9 h, 12 h and 15 h reperfusion subgroups were higher than each control group (p<0.05). The MPO activity of 6 h, 12 h and 15 h reperfusion subgroups were higher than each control group (p<0.05).

Conclusions: The neuroprotective effects of FNS have been shown to prolong the therapeutic window in cerebral ischemia/reperfusion, which might be related to the PPARγ mediated-inflammation in penumbral region.

No MeSH data available.


Related in: MedlinePlus