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Inhibition of calcium oxalate crystallisation in vitro by an extract of Bergenia ciliata.

Saha S, Verma RJ - Arab J Urol (2013)

Bottom Line: The results were compared with a parallel study conducted with a marketed poly-herbal combination, Cystone, under identical concentrations.The extract of B. ciliata was significantly more effective in inhibiting the nucleation and aggregation of COM crystals in a dose-dependent manner than was Cystone.Moreover, the extract induced more CaOx dihydrate crystals, with a significant reduction in the number and size of COM crystals.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad 380009, India.

ABSTRACT

Objective: To evaluate the effectiveness of an extract obtained from the rhizomes of Bergenia ciliata (Saxifragaceae) on the inhibition of calcium oxalate (CaOx) crystallisation in vitro.

Materials and methods: A hydro-alcoholic extract (30:70, v/v) of rhizomes of B. ciliata was prepared at different concentrations (1-10 mg/mL). The crystallisation of CaOx monohydrate (COM) was induced in a synthetic urine system. The nucleation and aggregation of COM crystals were measured using spectrophotometric methods. The rates of nucleation and aggregation were evaluated by comparing the slope of the turbidity of a control system with that of one exposed to the extract. The results were compared with a parallel study conducted with a marketed poly-herbal combination, Cystone, under identical concentrations. Crystals generated in the urine were also analysed by light microscopy. Statistical differences and percentage inhibitions were calculated and assessed.

Results: The extract of B. ciliata was significantly more effective in inhibiting the nucleation and aggregation of COM crystals in a dose-dependent manner than was Cystone. Moreover, the extract induced more CaOx dihydrate crystals, with a significant reduction in the number and size of COM crystals.

Conclusion: An extract of the traditional herb B. ciliata has an excellent inhibitory activity on crystalluria and therefore might be beneficial in dissolving urinary stones. However, further study in animal models of urolithiasis is needed to evaluate its potential anti-urolithiatic activity.

No MeSH data available.


Related in: MedlinePlus

The plot of turbidity as influenced by B. ciliata extract and Cystone at various concentrations.
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f0020: The plot of turbidity as influenced by B. ciliata extract and Cystone at various concentrations.

Mentions: The extract of B. ciliata at various concentrations also had a significant inhibitory effect on nucleation and aggregation, compared with Cystone. In the nucleation assay the number of crystals formed was estimated as the turbidity of the solution. The absorbance of the control was subtracted from that obtained with the extract. There was a steep decrease in the absorbance with increasing concentration of the extract (Fig. 4). The percentage inhibition of the extract was 32–92%, whereas with cystone it was 17–62% (Fig. 5A and B, Table 1). The dose-dependent increase in percentage inhibition of nucleation by the extract had a coefficient of regression (r2) of 0.965. The IC50 of the plant extract was 2.3 mg/mL, compared with 8.1 mg/mL for Cystone.


Inhibition of calcium oxalate crystallisation in vitro by an extract of Bergenia ciliata.

Saha S, Verma RJ - Arab J Urol (2013)

The plot of turbidity as influenced by B. ciliata extract and Cystone at various concentrations.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4443001&req=5

f0020: The plot of turbidity as influenced by B. ciliata extract and Cystone at various concentrations.
Mentions: The extract of B. ciliata at various concentrations also had a significant inhibitory effect on nucleation and aggregation, compared with Cystone. In the nucleation assay the number of crystals formed was estimated as the turbidity of the solution. The absorbance of the control was subtracted from that obtained with the extract. There was a steep decrease in the absorbance with increasing concentration of the extract (Fig. 4). The percentage inhibition of the extract was 32–92%, whereas with cystone it was 17–62% (Fig. 5A and B, Table 1). The dose-dependent increase in percentage inhibition of nucleation by the extract had a coefficient of regression (r2) of 0.965. The IC50 of the plant extract was 2.3 mg/mL, compared with 8.1 mg/mL for Cystone.

Bottom Line: The results were compared with a parallel study conducted with a marketed poly-herbal combination, Cystone, under identical concentrations.The extract of B. ciliata was significantly more effective in inhibiting the nucleation and aggregation of COM crystals in a dose-dependent manner than was Cystone.Moreover, the extract induced more CaOx dihydrate crystals, with a significant reduction in the number and size of COM crystals.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad 380009, India.

ABSTRACT

Objective: To evaluate the effectiveness of an extract obtained from the rhizomes of Bergenia ciliata (Saxifragaceae) on the inhibition of calcium oxalate (CaOx) crystallisation in vitro.

Materials and methods: A hydro-alcoholic extract (30:70, v/v) of rhizomes of B. ciliata was prepared at different concentrations (1-10 mg/mL). The crystallisation of CaOx monohydrate (COM) was induced in a synthetic urine system. The nucleation and aggregation of COM crystals were measured using spectrophotometric methods. The rates of nucleation and aggregation were evaluated by comparing the slope of the turbidity of a control system with that of one exposed to the extract. The results were compared with a parallel study conducted with a marketed poly-herbal combination, Cystone, under identical concentrations. Crystals generated in the urine were also analysed by light microscopy. Statistical differences and percentage inhibitions were calculated and assessed.

Results: The extract of B. ciliata was significantly more effective in inhibiting the nucleation and aggregation of COM crystals in a dose-dependent manner than was Cystone. Moreover, the extract induced more CaOx dihydrate crystals, with a significant reduction in the number and size of COM crystals.

Conclusion: An extract of the traditional herb B. ciliata has an excellent inhibitory activity on crystalluria and therefore might be beneficial in dissolving urinary stones. However, further study in animal models of urolithiasis is needed to evaluate its potential anti-urolithiatic activity.

No MeSH data available.


Related in: MedlinePlus