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Inhibition of calcium oxalate crystallisation in vitro by an extract of Bergenia ciliata.

Saha S, Verma RJ - Arab J Urol (2013)

Bottom Line: The results were compared with a parallel study conducted with a marketed poly-herbal combination, Cystone, under identical concentrations.The extract of B. ciliata was significantly more effective in inhibiting the nucleation and aggregation of COM crystals in a dose-dependent manner than was Cystone.Moreover, the extract induced more CaOx dihydrate crystals, with a significant reduction in the number and size of COM crystals.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad 380009, India.

ABSTRACT

Objective: To evaluate the effectiveness of an extract obtained from the rhizomes of Bergenia ciliata (Saxifragaceae) on the inhibition of calcium oxalate (CaOx) crystallisation in vitro.

Materials and methods: A hydro-alcoholic extract (30:70, v/v) of rhizomes of B. ciliata was prepared at different concentrations (1-10 mg/mL). The crystallisation of CaOx monohydrate (COM) was induced in a synthetic urine system. The nucleation and aggregation of COM crystals were measured using spectrophotometric methods. The rates of nucleation and aggregation were evaluated by comparing the slope of the turbidity of a control system with that of one exposed to the extract. The results were compared with a parallel study conducted with a marketed poly-herbal combination, Cystone, under identical concentrations. Crystals generated in the urine were also analysed by light microscopy. Statistical differences and percentage inhibitions were calculated and assessed.

Results: The extract of B. ciliata was significantly more effective in inhibiting the nucleation and aggregation of COM crystals in a dose-dependent manner than was Cystone. Moreover, the extract induced more CaOx dihydrate crystals, with a significant reduction in the number and size of COM crystals.

Conclusion: An extract of the traditional herb B. ciliata has an excellent inhibitory activity on crystalluria and therefore might be beneficial in dissolving urinary stones. However, further study in animal models of urolithiasis is needed to evaluate its potential anti-urolithiatic activity.

No MeSH data available.


Related in: MedlinePlus

B. ciliata (10 mg/mL) inhibited the crystallisation of CaOx (light microscopy × 100).
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f0010: B. ciliata (10 mg/mL) inhibited the crystallisation of CaOx (light microscopy × 100).

Mentions: The in vitro inhibitory effect of extracts of B. ciliata on various phases of CaOx crystallisation was determined by the time course of turbidity measured in synthetic urine at extract concentrations of 1, 2, 5, 7.5 and 10 mg/mL. The light micrographs at 6 h in the control system showed the formation of both types of CaOx crystals, as bi-concave, oval and dumb-bell shaped COM and octahedral COD, with significant aggregations (Fig. 1). The arrows in Fig. 1 show both types of crystals, but B. ciliata extract at the highest concentration (10 mg/mL) inhibited crystal formation, with no COM crystals and a few COD crystals, as shown by the arrows (Fig. 2). Moreover, there were fewer COD crystals with the extract. Cystone was less effective than the extract, with numerous COM and COD crystals when used at the same concentrations (Fig. 3), with the arrows showing a moderate number of COD crystals and a few COM crystals. Thus, the highest concentration of the B. ciliate extract was associated with only COD crystals.


Inhibition of calcium oxalate crystallisation in vitro by an extract of Bergenia ciliata.

Saha S, Verma RJ - Arab J Urol (2013)

B. ciliata (10 mg/mL) inhibited the crystallisation of CaOx (light microscopy × 100).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4443001&req=5

f0010: B. ciliata (10 mg/mL) inhibited the crystallisation of CaOx (light microscopy × 100).
Mentions: The in vitro inhibitory effect of extracts of B. ciliata on various phases of CaOx crystallisation was determined by the time course of turbidity measured in synthetic urine at extract concentrations of 1, 2, 5, 7.5 and 10 mg/mL. The light micrographs at 6 h in the control system showed the formation of both types of CaOx crystals, as bi-concave, oval and dumb-bell shaped COM and octahedral COD, with significant aggregations (Fig. 1). The arrows in Fig. 1 show both types of crystals, but B. ciliata extract at the highest concentration (10 mg/mL) inhibited crystal formation, with no COM crystals and a few COD crystals, as shown by the arrows (Fig. 2). Moreover, there were fewer COD crystals with the extract. Cystone was less effective than the extract, with numerous COM and COD crystals when used at the same concentrations (Fig. 3), with the arrows showing a moderate number of COD crystals and a few COM crystals. Thus, the highest concentration of the B. ciliate extract was associated with only COD crystals.

Bottom Line: The results were compared with a parallel study conducted with a marketed poly-herbal combination, Cystone, under identical concentrations.The extract of B. ciliata was significantly more effective in inhibiting the nucleation and aggregation of COM crystals in a dose-dependent manner than was Cystone.Moreover, the extract induced more CaOx dihydrate crystals, with a significant reduction in the number and size of COM crystals.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, University School of Sciences, Gujarat University, Ahmedabad 380009, India.

ABSTRACT

Objective: To evaluate the effectiveness of an extract obtained from the rhizomes of Bergenia ciliata (Saxifragaceae) on the inhibition of calcium oxalate (CaOx) crystallisation in vitro.

Materials and methods: A hydro-alcoholic extract (30:70, v/v) of rhizomes of B. ciliata was prepared at different concentrations (1-10 mg/mL). The crystallisation of CaOx monohydrate (COM) was induced in a synthetic urine system. The nucleation and aggregation of COM crystals were measured using spectrophotometric methods. The rates of nucleation and aggregation were evaluated by comparing the slope of the turbidity of a control system with that of one exposed to the extract. The results were compared with a parallel study conducted with a marketed poly-herbal combination, Cystone, under identical concentrations. Crystals generated in the urine were also analysed by light microscopy. Statistical differences and percentage inhibitions were calculated and assessed.

Results: The extract of B. ciliata was significantly more effective in inhibiting the nucleation and aggregation of COM crystals in a dose-dependent manner than was Cystone. Moreover, the extract induced more CaOx dihydrate crystals, with a significant reduction in the number and size of COM crystals.

Conclusion: An extract of the traditional herb B. ciliata has an excellent inhibitory activity on crystalluria and therefore might be beneficial in dissolving urinary stones. However, further study in animal models of urolithiasis is needed to evaluate its potential anti-urolithiatic activity.

No MeSH data available.


Related in: MedlinePlus