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Analysis of Genomic Diversity among Helicobacter pylori Strains Isolated from Iranian Children by Pulsed Field Gel Electrophoresis.

Falsafi T, Sotoudeh N, Feizabadi MM, Mahjoub F - Iran J Pediatr (2014)

Bottom Line: Similar conclusion was obtained by Total Lab software.However, more relationship was observed between the strains isolated in the close period (1997-2009, 2001-2003, 2005-2007, and 2007-2009) and more difference was observed among those obtained in the distant periods (1997 and 2009).H. pylori strains isolated from children in Iran are extremely diverse and this diversity is not related to their virulence characteristics.

View Article: PubMed Central - PubMed

Affiliation: Department of Mirobiology, Faculty of Biology, Alzahra University, Tehran.

ABSTRACT

Objective: Presence of genomic diversity among Helicobacter pylori (H. pylori) strains have been suggested by numerous investigators. Little is known about diversity of H. pylori strains isolated from Iranian children and their association with virulence of the strains. Our purpose was to assess the degree of genomic diversity among H. pylori strains isolated from Iranian-children, on the basis of vacA genotype, cagA status of the strains, sex, age as well as the pathological status of the patients.

Methods: Genomic DNA from 44 unrelated H. pylori strains isolated during 1997-2009, was examined by pulse-field gel electrophoresis (PFGE). Pathological status of the patients was performed according to the modified Sydney-system and genotype/status of vacA/cagA genes was determined by PCR. PFGE was performed using XbaI restriction-endonuclease and the field inversion-gel electrophoresis system.

Findings: No significant relationship was observed between the patterns of PFGE and the cagA/vacA status/genotype. Also no relationship was observed between age, sex, and pathological status of the children and the PFGE patterns of their isolates. Similar conclusion was obtained by Total Lab software. However, more relationship was observed between the strains isolated in the close period (1997-2009, 2001-2003, 2005-2007, and 2007-2009) and more difference was observed among those obtained in the distant periods (1997 and 2009).

Conclusion: H. pylori strains isolated from children in Iran are extremely diverse and this diversity is not related to their virulence characteristics. Occurrence of this extreme diversity may be related to adaptation of H. pylori strains to variable living conditions during transmission between various host individuals.

No MeSH data available.


Related in: MedlinePlus

PFGE profile of isolates of group III.Numbers above figures represent the number of strains according to Table 1.
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F2b: PFGE profile of isolates of group III.Numbers above figures represent the number of strains according to Table 1.

Mentions: Comparison of the PFGE patterns suggested that genomic diversity of the strains was not related to the genotype of vacA, status of cagA and the status of gastric inflammation in children (Fig. 1–2, Table 2). The fact that identical PFGE pattern did not occur in the strains with similar pathogenicity-associated markers such as cagA and vacA may suggest that the DNA fragments undergoing genetic changes did not encode proteins that are involved in virulence. Comparison of multiple H. pylori strains by different methods have shown that H. pylori genome has highly plastic gene content and nearly half of the strain-specific genes may be located in each region[34].


Analysis of Genomic Diversity among Helicobacter pylori Strains Isolated from Iranian Children by Pulsed Field Gel Electrophoresis.

Falsafi T, Sotoudeh N, Feizabadi MM, Mahjoub F - Iran J Pediatr (2014)

PFGE profile of isolates of group III.Numbers above figures represent the number of strains according to Table 1.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4442831&req=5

F2b: PFGE profile of isolates of group III.Numbers above figures represent the number of strains according to Table 1.
Mentions: Comparison of the PFGE patterns suggested that genomic diversity of the strains was not related to the genotype of vacA, status of cagA and the status of gastric inflammation in children (Fig. 1–2, Table 2). The fact that identical PFGE pattern did not occur in the strains with similar pathogenicity-associated markers such as cagA and vacA may suggest that the DNA fragments undergoing genetic changes did not encode proteins that are involved in virulence. Comparison of multiple H. pylori strains by different methods have shown that H. pylori genome has highly plastic gene content and nearly half of the strain-specific genes may be located in each region[34].

Bottom Line: Similar conclusion was obtained by Total Lab software.However, more relationship was observed between the strains isolated in the close period (1997-2009, 2001-2003, 2005-2007, and 2007-2009) and more difference was observed among those obtained in the distant periods (1997 and 2009).H. pylori strains isolated from children in Iran are extremely diverse and this diversity is not related to their virulence characteristics.

View Article: PubMed Central - PubMed

Affiliation: Department of Mirobiology, Faculty of Biology, Alzahra University, Tehran.

ABSTRACT

Objective: Presence of genomic diversity among Helicobacter pylori (H. pylori) strains have been suggested by numerous investigators. Little is known about diversity of H. pylori strains isolated from Iranian children and their association with virulence of the strains. Our purpose was to assess the degree of genomic diversity among H. pylori strains isolated from Iranian-children, on the basis of vacA genotype, cagA status of the strains, sex, age as well as the pathological status of the patients.

Methods: Genomic DNA from 44 unrelated H. pylori strains isolated during 1997-2009, was examined by pulse-field gel electrophoresis (PFGE). Pathological status of the patients was performed according to the modified Sydney-system and genotype/status of vacA/cagA genes was determined by PCR. PFGE was performed using XbaI restriction-endonuclease and the field inversion-gel electrophoresis system.

Findings: No significant relationship was observed between the patterns of PFGE and the cagA/vacA status/genotype. Also no relationship was observed between age, sex, and pathological status of the children and the PFGE patterns of their isolates. Similar conclusion was obtained by Total Lab software. However, more relationship was observed between the strains isolated in the close period (1997-2009, 2001-2003, 2005-2007, and 2007-2009) and more difference was observed among those obtained in the distant periods (1997 and 2009).

Conclusion: H. pylori strains isolated from children in Iran are extremely diverse and this diversity is not related to their virulence characteristics. Occurrence of this extreme diversity may be related to adaptation of H. pylori strains to variable living conditions during transmission between various host individuals.

No MeSH data available.


Related in: MedlinePlus