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A Systematic Meta-analysis of Immune Signatures in Patients With Acute Chikungunya Virus Infection.

Teng TS, Kam YW, Lee B, Hapuarachchi HC, Wimal A, Ng LC, Ng LF - J. Infect. Dis. (2015)

Bottom Line: However, studies in different patient cohorts have revealed that disease manifestations vary in frequency.Our findings revealed a specific pattern of clinical presentations of joint-specific arthralgia from this CHIKV cohort.More importantly, we identified an immune mediator signature dominated by proinflammatory cytokines, which include interferon α and γ and interleukin 2, 2R, 6, 7, 12, 15, 17, and 18, across different patient cohorts of CHIKV load associated with arthralgia.

View Article: PubMed Central - PubMed

Affiliation: Singapore Immunology Network, Agency for Science, Technology and Research (A*STAR), Biopolis.

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Related in: MedlinePlus

Heat map of immune mediator profiles collected by meta-analysis [20–27, 29– 33]. The profiles of all the immune mediators analyzed in the studies included in Figure 3 were represented by the heat map according to their sample types and geographic locations (in a west-to-east orientation) before being classified as chemokines (A) or growth factors (B). Each colored well in the 4 heat maps represents the relative levels of each immune mediator. Yellow indicates low expression; blue, high expression; gray, immune mediators not investigated in the studies. Abbreviations: EGF, epidermal growth factor; G-CSF, granulocyte colony-stimulating factor; HGF, hepatocyte growth factor; IP-10, interferon gamma-induced protein 10; MCF, monocyte chemoattractant protein; MIG, monokine induced by gamma interferon; MIP, macrophage inflammatory protein.
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JIV049F4: Heat map of immune mediator profiles collected by meta-analysis [20–27, 29– 33]. The profiles of all the immune mediators analyzed in the studies included in Figure 3 were represented by the heat map according to their sample types and geographic locations (in a west-to-east orientation) before being classified as chemokines (A) or growth factors (B). Each colored well in the 4 heat maps represents the relative levels of each immune mediator. Yellow indicates low expression; blue, high expression; gray, immune mediators not investigated in the studies. Abbreviations: EGF, epidermal growth factor; G-CSF, granulocyte colony-stimulating factor; HGF, hepatocyte growth factor; IP-10, interferon gamma-induced protein 10; MCF, monocyte chemoattractant protein; MIG, monokine induced by gamma interferon; MIP, macrophage inflammatory protein.

Mentions: Of the 14 studies identified, 5 were excluded from the meta-analysis because their data could not be extracted for analysis: in 1 study, the results from the cytokine assay were presented as optical density readings and could not be computed into concentration values [26]; another study contained data from only a single patient [28]. To overcome the lack of immune mediator profiles from non-CHIKV controls in this study, we used the data from healthy controls in a previously published study [24] as a reference to perform the analysis. A heat map was generated to provide an overall view of the expression profiles of all immune mediators analyzed in the 12 studies, together with data from the current Sri Lankan patient cohort. The immune mediators were segregated according to their functions as proinflammatory cytokines (Figure 3A), anti-inflammatory cytokines (Figure 3B), chemokines (Figure 4A), and growth factors (Figure 4B).Figure 3.


A Systematic Meta-analysis of Immune Signatures in Patients With Acute Chikungunya Virus Infection.

Teng TS, Kam YW, Lee B, Hapuarachchi HC, Wimal A, Ng LC, Ng LF - J. Infect. Dis. (2015)

Heat map of immune mediator profiles collected by meta-analysis [20–27, 29– 33]. The profiles of all the immune mediators analyzed in the studies included in Figure 3 were represented by the heat map according to their sample types and geographic locations (in a west-to-east orientation) before being classified as chemokines (A) or growth factors (B). Each colored well in the 4 heat maps represents the relative levels of each immune mediator. Yellow indicates low expression; blue, high expression; gray, immune mediators not investigated in the studies. Abbreviations: EGF, epidermal growth factor; G-CSF, granulocyte colony-stimulating factor; HGF, hepatocyte growth factor; IP-10, interferon gamma-induced protein 10; MCF, monocyte chemoattractant protein; MIG, monokine induced by gamma interferon; MIP, macrophage inflammatory protein.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4442625&req=5

JIV049F4: Heat map of immune mediator profiles collected by meta-analysis [20–27, 29– 33]. The profiles of all the immune mediators analyzed in the studies included in Figure 3 were represented by the heat map according to their sample types and geographic locations (in a west-to-east orientation) before being classified as chemokines (A) or growth factors (B). Each colored well in the 4 heat maps represents the relative levels of each immune mediator. Yellow indicates low expression; blue, high expression; gray, immune mediators not investigated in the studies. Abbreviations: EGF, epidermal growth factor; G-CSF, granulocyte colony-stimulating factor; HGF, hepatocyte growth factor; IP-10, interferon gamma-induced protein 10; MCF, monocyte chemoattractant protein; MIG, monokine induced by gamma interferon; MIP, macrophage inflammatory protein.
Mentions: Of the 14 studies identified, 5 were excluded from the meta-analysis because their data could not be extracted for analysis: in 1 study, the results from the cytokine assay were presented as optical density readings and could not be computed into concentration values [26]; another study contained data from only a single patient [28]. To overcome the lack of immune mediator profiles from non-CHIKV controls in this study, we used the data from healthy controls in a previously published study [24] as a reference to perform the analysis. A heat map was generated to provide an overall view of the expression profiles of all immune mediators analyzed in the 12 studies, together with data from the current Sri Lankan patient cohort. The immune mediators were segregated according to their functions as proinflammatory cytokines (Figure 3A), anti-inflammatory cytokines (Figure 3B), chemokines (Figure 4A), and growth factors (Figure 4B).Figure 3.

Bottom Line: However, studies in different patient cohorts have revealed that disease manifestations vary in frequency.Our findings revealed a specific pattern of clinical presentations of joint-specific arthralgia from this CHIKV cohort.More importantly, we identified an immune mediator signature dominated by proinflammatory cytokines, which include interferon α and γ and interleukin 2, 2R, 6, 7, 12, 15, 17, and 18, across different patient cohorts of CHIKV load associated with arthralgia.

View Article: PubMed Central - PubMed

Affiliation: Singapore Immunology Network, Agency for Science, Technology and Research (A*STAR), Biopolis.

Show MeSH
Related in: MedlinePlus