Alveolar Macrophages Isolated Directly From Human Cytomegalovirus (HCMV)-Seropositive Individuals Are Sites of HCMV Reactivation In Vivo.
Bottom Line: Human cytomegalovirus (HCMV) causes significant morbidity in the immunocompromised host.This has been used to support the concept that viral reactivation in HCMV carriers routinely occurs from such terminally differentiated myeloid cells in vivo.However, to date this has not been shown for in vivo-differentiated macrophages.
Affiliation: Department of Medicine, University of Cambridge, United Kingdom.Show MeSH
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Mentions: To determine whether alveolar macrophages are sites of reactivation of full virus production, they were cocultured on indicator fibroblasts. After 3 weeks of coculture, the fibroblasts were fixed and stained for detection of infection foci where IE was being expressed. Figure 4A shows that foci of infection were clearly identifiable as areas of swollen cells within the fibroblast monolayer. Figure 4B shows that less than one in a million alveolar macrophages from seropositive individuals are productive for viral spread in vitro. Finally, to demonstrate that virions were being released into the supernatant, the supernatants were collected and transferred onto fresh fibroblasts, and the numbers of IE-expressing cells were enumerated over time (Figure 4C).Figure 4.
Affiliation: Department of Medicine, University of Cambridge, United Kingdom.