Alveolar Macrophages Isolated Directly From Human Cytomegalovirus (HCMV)-Seropositive Individuals Are Sites of HCMV Reactivation In Vivo.
Bottom Line: Human cytomegalovirus (HCMV) causes significant morbidity in the immunocompromised host.This has been used to support the concept that viral reactivation in HCMV carriers routinely occurs from such terminally differentiated myeloid cells in vivo.However, to date this has not been shown for in vivo-differentiated macrophages.
Affiliation: Department of Medicine, University of Cambridge, United Kingdom.Show MeSH
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Mentions: We and others have previously shown that latent infection in healthy seropositive virus carriers can be assessed by the analysis of peripheral monocytes for HCMV lytic gene expression . To ensure that the seropositive donor was latently infected, CD14+ monocytes were isolated from blood and analyzed for the standard hallmarks of latency: the presence of latent transcripts and the absence of lytic gene transcription. Figure 3A shows that CD14+ cells isolated from venous blood of the seropositive donor expressed the latency-associated transcript UL138 in the absence of expression of the lytic IE gene. We did not observe viral gene expression in monocytes from the seronegative donor. Alveolar macrophages from both donors were stained for detection of IE; IE expression was detectable in a few cells from the seropositive donor (Figure 3B) but in no cells from the seronegative donor (data not shown).Figure 3.
Affiliation: Department of Medicine, University of Cambridge, United Kingdom.