Multiple genome segments determine virulence of bluetongue virus serotype 8.
Bottom Line: We found that partial attenuation of BTV8L in IFNAR(-/-) mice was achieved by simply replacing genomic segment 2 (Seg2, encoding VP2) or Seg10 (encoding NS3) with the BTV8H homologous segments.Replication of BTV8H was relatively poor in interferon (IFN)-competent primary ovine endothelial cells compared to replication of BTV8L, and this phenotype was determined by several viral genomic segments, including Seg4 and Seg9.The possibility to determine the pathogenicity of virus isolates on the basis of their genome sequences will help in the design of control strategies that fit the risk posed by new emerging BTV strains.
Affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom.Show MeSH
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Mentions: Next, we wanted to establish whether BTV8H was a more potent IFN inducer than the parental BTV8L. To this end, we measured IFN production in the supernatants of OvEC infected with the same set of viruses as above. rgBTV8H and most of the monoreassortants induced similar amounts of IFN. However, statistically significant differences (P < 0.05) were observed between rgBTV8L and BTV8L+S9H (Fig. 7A).
Affiliation: MRC-University of Glasgow Centre for Virus Research, Glasgow, United Kingdom.