Genome diversity of Epstein-Barr virus from multiple tumor types and normal infection.
Bottom Line: Our results provide the first global view of EBV sequence variation and demonstrate an effective method for sequencing large numbers of genomes to further understand the genetics of EBV infection.Here we used rapid, cost-effective sequencing to determine 71 new EBV sequences from different sample types and locations worldwide.This gives the first overview of EBV genome variation, important for designing vaccines and immune therapy for EBV, and provides techniques to investigate relationships between viral sequence variation and EBV-associated diseases.
Affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom.Show MeSH
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Mentions: The multiple-sequence alignment (Fig. 1B) and detailed gene-by-gene analysis of all 83 EBV genomes strongly suggests that the current EBV genome map annotated in NC_007605 is a good representation of the EBV genome. Consistent with this, the open reading frames were shown to be conserved (Fig. 2B). The EBV genome Saliva1 is derived directly from saliva of a healthy carrier and is the first wild-type EBV genome sequenced that was not selected by immortalization of B cells or derived from a cancer cell (Fig. 1B). The close agreement of this sequence to the NC_007605 reference sequence, with no additional insertions or deletions, indicates that the standard EBV genome map is representative of transmissible saliva strains of EBV. The closest EBV strain (based on the fewest SNPs) to the saliva EBV was HKN19, a spontaneous LCL from Hong Kong. Although the identities of the saliva donors tested were anonymous, the panel did include some Asian donors, so it is likely that this is the basis of the similarity.
Affiliation: Wellcome Trust Sanger Institute, Hinxton, Cambridge, United Kingdom.