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Evaluation of antioxidant producing potential of halophilic bacterial bionts from marine invertebrates.

Velho-Pereira S, Parvatkar P, Furtado IJ - Indian J Pharm Sci (2015 Mar-Apr)

Bottom Line: Methanol extract of biont GUVFCFM-3 produced 67.83% scavenging of 2,2-diphenyl-2-picrylhydrazyl free radicals and 65.87% scavenging of superoxide free radicals.Preliminary tests leading to the identification of the extracellular antioxidant factor produced by GUVFCFM-3 revealed that it is a peptide.We report that the genera Chromohalobacter sp. primarily known for its unique salt tolerating abilities by virtue of the production of osmolytes is an excellent scavenger of free radicals.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Goa University, Taleigao Plateau, Goa-403 203, India.

ABSTRACT
Marine invertebrates exposed to high levels of reactive oxygen species in the oceans have been reported to produce antioxidants as a major defense against free radical mediated toxicity; protecting their tissues from the damage associated with the oxidative stress. In view of this, the present study was carried out to determine the antioxidant activity of 100 bacterial bionts isolated from marine sponges, corals and a single bivalve. Methanol extract of biont GUVFCFM-3 produced 67.83% scavenging of 2,2-diphenyl-2-picrylhydrazyl free radicals and 65.87% scavenging of superoxide free radicals. Preliminary tests leading to the identification of the extracellular antioxidant factor produced by GUVFCFM-3 revealed that it is a peptide. We report that the genera Chromohalobacter sp. primarily known for its unique salt tolerating abilities by virtue of the production of osmolytes is an excellent scavenger of free radicals.

No MeSH data available.


Related in: MedlinePlus

DPPH and Superoxide free radical scavenging activity of crude extracts of bacterial biont.DPPH, ▪superoxide.
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Figure 2: DPPH and Superoxide free radical scavenging activity of crude extracts of bacterial biont.DPPH, ▪superoxide.

Mentions: Among the sponge bionts, GUVFCFM-3 (bearing accession number JX839255) showed the highest percentage scavenging at 67.05% (fig. 2) and was identified as C. israelensis. Among the coral bionts, GUVFEIM-3 identified as C. israelensis (bearing accession number JX839277) showed the highest percentage scavenging at 63.77%. It was followed closely by biont GUVFEIM-7 (bearing accession number JX839263) identified again as C. israelensis at 63.52%. Amongst the bivalve bionts isolate GUVFPVM-3 identified as S. luteus (bearing accession number JX839272) showed the highest percentage scavenging at 65.21% followed by GUVFPVM-5 identified as P. celer (bearing accession number JX839269) at 64.17%. In our study, we found that the extract of sponge biont C. israelensis (GUVFCFM-3) showed the maximum DPPH radical scavenging activity 67.05% among all the other extracts tested at a similar concentration of 1000 μg/ml while the lowest was seen in the coral biont V. salexigens (GUVFEIM-4) (bearing accession number JX839261) at 28.56%.


Evaluation of antioxidant producing potential of halophilic bacterial bionts from marine invertebrates.

Velho-Pereira S, Parvatkar P, Furtado IJ - Indian J Pharm Sci (2015 Mar-Apr)

DPPH and Superoxide free radical scavenging activity of crude extracts of bacterial biont.DPPH, ▪superoxide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4442467&req=5

Figure 2: DPPH and Superoxide free radical scavenging activity of crude extracts of bacterial biont.DPPH, ▪superoxide.
Mentions: Among the sponge bionts, GUVFCFM-3 (bearing accession number JX839255) showed the highest percentage scavenging at 67.05% (fig. 2) and was identified as C. israelensis. Among the coral bionts, GUVFEIM-3 identified as C. israelensis (bearing accession number JX839277) showed the highest percentage scavenging at 63.77%. It was followed closely by biont GUVFEIM-7 (bearing accession number JX839263) identified again as C. israelensis at 63.52%. Amongst the bivalve bionts isolate GUVFPVM-3 identified as S. luteus (bearing accession number JX839272) showed the highest percentage scavenging at 65.21% followed by GUVFPVM-5 identified as P. celer (bearing accession number JX839269) at 64.17%. In our study, we found that the extract of sponge biont C. israelensis (GUVFCFM-3) showed the maximum DPPH radical scavenging activity 67.05% among all the other extracts tested at a similar concentration of 1000 μg/ml while the lowest was seen in the coral biont V. salexigens (GUVFEIM-4) (bearing accession number JX839261) at 28.56%.

Bottom Line: Methanol extract of biont GUVFCFM-3 produced 67.83% scavenging of 2,2-diphenyl-2-picrylhydrazyl free radicals and 65.87% scavenging of superoxide free radicals.Preliminary tests leading to the identification of the extracellular antioxidant factor produced by GUVFCFM-3 revealed that it is a peptide.We report that the genera Chromohalobacter sp. primarily known for its unique salt tolerating abilities by virtue of the production of osmolytes is an excellent scavenger of free radicals.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Goa University, Taleigao Plateau, Goa-403 203, India.

ABSTRACT
Marine invertebrates exposed to high levels of reactive oxygen species in the oceans have been reported to produce antioxidants as a major defense against free radical mediated toxicity; protecting their tissues from the damage associated with the oxidative stress. In view of this, the present study was carried out to determine the antioxidant activity of 100 bacterial bionts isolated from marine sponges, corals and a single bivalve. Methanol extract of biont GUVFCFM-3 produced 67.83% scavenging of 2,2-diphenyl-2-picrylhydrazyl free radicals and 65.87% scavenging of superoxide free radicals. Preliminary tests leading to the identification of the extracellular antioxidant factor produced by GUVFCFM-3 revealed that it is a peptide. We report that the genera Chromohalobacter sp. primarily known for its unique salt tolerating abilities by virtue of the production of osmolytes is an excellent scavenger of free radicals.

No MeSH data available.


Related in: MedlinePlus