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In vitro Antioxidant and Pharmacognostic Studies of Leaf Extracts of Cajanus cajan (L.) Millsp.

Mahitha B, Archana P, Ebrahimzadeh MH, Srikanth K, Rajinikanth M, Ramaswamy N - Indian J Pharm Sci (2015 Mar-Apr)

Bottom Line: Physicochemical parameters such as total, acid-insoluble and water-soluble ash values and moisture content of the leaf powder of C. cajan were found to be 9.50%, 1.40 g/100 g, 4.15 g/100 g drug and 6.72%, respectively.The results of the antioxidant activity were found to be concentration-dependent.Microscopic study showed the presence of collenchyma, fibres, xylem, phloem, epidermis, trichomes, palisade tissue, basal sheath, pith and cortex in leaf, petiole and pulvinus.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Research Group, Department of Biotechnology, Kakatiya University, Warangal-506 009, India.

ABSTRACT
Cajanus cajan (L.) Millsp is one of the second most dietary legume crops. The leaf extracts may be used as a potential source of natural antioxidant. The ash values, extractive values, total phenolic and flavonoid content, in vitro antioxidant activity of various leaf extracts as well as anatomical investigation of Cajanus cajan were carried out. Physicochemical parameters such as total, acid-insoluble and water-soluble ash values and moisture content of the leaf powder of C. cajan were found to be 9.50%, 1.40 g/100 g, 4.15 g/100 g drug and 6.72%, respectively. Percent yield of acetone, aqueous, ethanol, ethyl acetate and chloroform leaf extracts were 9.0, 10.6, 13.75, 8.7 and 5.8 g/100 g, respectively. Significant amount of phenolic and flavonoid content were observed. The results of the antioxidant activity were found to be concentration-dependent. The IC50 values for DPPH assay determined for aqueous and ethanol extracts were 0.69 and 0.79 mg/ml, respectively. Reducing power is increased with increasing amount of concentration in both aqueous and ethanol leaf extracts. The highest hydroxyl radical scavenging activity reached up to 83.67% in aqueous and 78.75% in ethanol extracts and in phosphomolybdenum assay the aqueous extract showed strong antioxidant capacity up to 55.97 nM gallic acid equivalents/g. It was found that the aqueous extract possessed highest antioxidant activity in all the assays tested. The antioxidant characteristics of leaf extracts are possibly because of the presence of polyphenols. Microscopic study showed the presence of collenchyma, fibres, xylem, phloem, epidermis, trichomes, palisade tissue, basal sheath, pith and cortex in leaf, petiole and pulvinus.

No MeSH data available.


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Phosphomolybdenum assay of various leaf extracts of C. cajan.
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Figure 5: Phosphomolybdenum assay of various leaf extracts of C. cajan.

Mentions: To assess the antioxidant capacity by phosphomolybdenum assay the basic principle implied is the reduction of molybdenum forms a green molybdenum complex by the antioxidant compounds present in the plant extracts which has absorption at 695 nm[43]. The data on total antioxidant capacity observed in acetone, aqueous, ethanol, ethyl acetate and chloroform leaf extracts of C. cajan are shown in fig. 5. The antioxidant activity of phosphomolybdenum assay was found in this order: aqueous>ethanol>ethyl acetate>chloroform>acetone. Among the extracts tested, aqueous extract showed highest antioxidant activity in comparison to others.


In vitro Antioxidant and Pharmacognostic Studies of Leaf Extracts of Cajanus cajan (L.) Millsp.

Mahitha B, Archana P, Ebrahimzadeh MH, Srikanth K, Rajinikanth M, Ramaswamy N - Indian J Pharm Sci (2015 Mar-Apr)

Phosphomolybdenum assay of various leaf extracts of C. cajan.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4442465&req=5

Figure 5: Phosphomolybdenum assay of various leaf extracts of C. cajan.
Mentions: To assess the antioxidant capacity by phosphomolybdenum assay the basic principle implied is the reduction of molybdenum forms a green molybdenum complex by the antioxidant compounds present in the plant extracts which has absorption at 695 nm[43]. The data on total antioxidant capacity observed in acetone, aqueous, ethanol, ethyl acetate and chloroform leaf extracts of C. cajan are shown in fig. 5. The antioxidant activity of phosphomolybdenum assay was found in this order: aqueous>ethanol>ethyl acetate>chloroform>acetone. Among the extracts tested, aqueous extract showed highest antioxidant activity in comparison to others.

Bottom Line: Physicochemical parameters such as total, acid-insoluble and water-soluble ash values and moisture content of the leaf powder of C. cajan were found to be 9.50%, 1.40 g/100 g, 4.15 g/100 g drug and 6.72%, respectively.The results of the antioxidant activity were found to be concentration-dependent.Microscopic study showed the presence of collenchyma, fibres, xylem, phloem, epidermis, trichomes, palisade tissue, basal sheath, pith and cortex in leaf, petiole and pulvinus.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Research Group, Department of Biotechnology, Kakatiya University, Warangal-506 009, India.

ABSTRACT
Cajanus cajan (L.) Millsp is one of the second most dietary legume crops. The leaf extracts may be used as a potential source of natural antioxidant. The ash values, extractive values, total phenolic and flavonoid content, in vitro antioxidant activity of various leaf extracts as well as anatomical investigation of Cajanus cajan were carried out. Physicochemical parameters such as total, acid-insoluble and water-soluble ash values and moisture content of the leaf powder of C. cajan were found to be 9.50%, 1.40 g/100 g, 4.15 g/100 g drug and 6.72%, respectively. Percent yield of acetone, aqueous, ethanol, ethyl acetate and chloroform leaf extracts were 9.0, 10.6, 13.75, 8.7 and 5.8 g/100 g, respectively. Significant amount of phenolic and flavonoid content were observed. The results of the antioxidant activity were found to be concentration-dependent. The IC50 values for DPPH assay determined for aqueous and ethanol extracts were 0.69 and 0.79 mg/ml, respectively. Reducing power is increased with increasing amount of concentration in both aqueous and ethanol leaf extracts. The highest hydroxyl radical scavenging activity reached up to 83.67% in aqueous and 78.75% in ethanol extracts and in phosphomolybdenum assay the aqueous extract showed strong antioxidant capacity up to 55.97 nM gallic acid equivalents/g. It was found that the aqueous extract possessed highest antioxidant activity in all the assays tested. The antioxidant characteristics of leaf extracts are possibly because of the presence of polyphenols. Microscopic study showed the presence of collenchyma, fibres, xylem, phloem, epidermis, trichomes, palisade tissue, basal sheath, pith and cortex in leaf, petiole and pulvinus.

No MeSH data available.


Related in: MedlinePlus