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Combating drug resistant pathogenic bacteria isolated from clinical infections, with silver oxide nanoparticles.

Sangappa M, Thiagarajan P - Indian J Pharm Sci (2015 Mar-Apr)

Bottom Line: Production of Ag2O nanoparticles was confirmed by color change of fungal filtrate and UV light absorption at 450 nm.Fourier transform infrared spectroscopy indicated the presence of primary amine, carbonyl group, NO2 and silver, revealing protein mediated nanoparticle production.The scanning electron microscope image showed freely dispersed Ag2O nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences and Technology, VIT University, Tamil Nadu, Vellore-632014, India.

ABSTRACT
The antibiogram study of methicillin resistant Staphylococcus aureus isolates revealed 100% resistance to vancomycin, bacitracin, erythromycin, ciprofloxacin and nalidixic acid. Eight isolates (53.3%) showed resistance to co-trimoxazole and one isolate to rifampicin, which was the drug of choice. An effort was made to evaluate the antimethicillin resistant Staphylococcus aureus activity of silver oxide (Ag2O) nanoparticles synthesized from Aspergillus terreus VIT 2013. Production of Ag2O nanoparticles was confirmed by color change of fungal filtrate and UV light absorption at 450 nm. X-ray diffraction pattern showed 2θ values at 27, 32, 38 and 57°, which corresponded to the cubic structure of Ag2O nanocrystals. Fourier transform infrared spectroscopy indicated the presence of primary amine, carbonyl group, NO2 and silver, revealing protein mediated nanoparticle production. The scanning electron microscope image showed freely dispersed Ag2O nanoparticles. The nanoparticles were active against all methicillin resistant isolates and hence can be used as antibacterial agents against drug resistant bacteria.

No MeSH data available.


Related in: MedlinePlus

Characterization of Ag2O nanoparticle production.Characterization of Ag2O nanoparticle production through change in filtrate color (a), UV spectra (b).
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Figure 1: Characterization of Ag2O nanoparticle production.Characterization of Ag2O nanoparticle production through change in filtrate color (a), UV spectra (b).

Mentions: Each isolate was resistant to more than one antibiotic, thus showing high versatility of drug resistance among nosocomial MRSA. Since drug resistance is disseminating at a rapid pace, it should be of serious concern for prevention and control of diseases caused by them. Nanotechnology is one of the promising areas to battle the drug resistance scenario. Silver-carbon complexes, nitric oxide releasing nanoparticles and other multifunctional nanoparticles have been reported to have strong antibacterial activities against drug resistance bacteria[14]. Antibacterial Ag2O nanoparticles synthesis, by soil fungus Aspergillus terreus VIT 2013 was carried out and the presence of Ag2O was confirmed through the characteristic color change in the fungal filtrate, from colorless to brown, upon addition of AgNO3. The color change was noticed within one hour of addition of the substrate. No change in color was observed in the remaining two conical flasks containing only fungal filtrate and 0.1 mM AgNO3 solution, which served as controls (fig. 1a). Test solution, when scanned from 300-600 nm showed strong UV peak absorption at 450 nm. The optical density (OD) value of each successive recording at 450 nm was approximately doubled which signified increased synthesis of Ag2O nanoparticles (fig. 1b). In contrast to Ag2O nanoparticles, silver (Ag°) nanoparticles absorbs light at 420 nm as observed in our previous study, thus clearly indicating a difference in UV absorption spectra of two nanoparticles[151617].


Combating drug resistant pathogenic bacteria isolated from clinical infections, with silver oxide nanoparticles.

Sangappa M, Thiagarajan P - Indian J Pharm Sci (2015 Mar-Apr)

Characterization of Ag2O nanoparticle production.Characterization of Ag2O nanoparticle production through change in filtrate color (a), UV spectra (b).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4442462&req=5

Figure 1: Characterization of Ag2O nanoparticle production.Characterization of Ag2O nanoparticle production through change in filtrate color (a), UV spectra (b).
Mentions: Each isolate was resistant to more than one antibiotic, thus showing high versatility of drug resistance among nosocomial MRSA. Since drug resistance is disseminating at a rapid pace, it should be of serious concern for prevention and control of diseases caused by them. Nanotechnology is one of the promising areas to battle the drug resistance scenario. Silver-carbon complexes, nitric oxide releasing nanoparticles and other multifunctional nanoparticles have been reported to have strong antibacterial activities against drug resistance bacteria[14]. Antibacterial Ag2O nanoparticles synthesis, by soil fungus Aspergillus terreus VIT 2013 was carried out and the presence of Ag2O was confirmed through the characteristic color change in the fungal filtrate, from colorless to brown, upon addition of AgNO3. The color change was noticed within one hour of addition of the substrate. No change in color was observed in the remaining two conical flasks containing only fungal filtrate and 0.1 mM AgNO3 solution, which served as controls (fig. 1a). Test solution, when scanned from 300-600 nm showed strong UV peak absorption at 450 nm. The optical density (OD) value of each successive recording at 450 nm was approximately doubled which signified increased synthesis of Ag2O nanoparticles (fig. 1b). In contrast to Ag2O nanoparticles, silver (Ag°) nanoparticles absorbs light at 420 nm as observed in our previous study, thus clearly indicating a difference in UV absorption spectra of two nanoparticles[151617].

Bottom Line: Production of Ag2O nanoparticles was confirmed by color change of fungal filtrate and UV light absorption at 450 nm.Fourier transform infrared spectroscopy indicated the presence of primary amine, carbonyl group, NO2 and silver, revealing protein mediated nanoparticle production.The scanning electron microscope image showed freely dispersed Ag2O nanoparticles.

View Article: PubMed Central - PubMed

Affiliation: School of Biosciences and Technology, VIT University, Tamil Nadu, Vellore-632014, India.

ABSTRACT
The antibiogram study of methicillin resistant Staphylococcus aureus isolates revealed 100% resistance to vancomycin, bacitracin, erythromycin, ciprofloxacin and nalidixic acid. Eight isolates (53.3%) showed resistance to co-trimoxazole and one isolate to rifampicin, which was the drug of choice. An effort was made to evaluate the antimethicillin resistant Staphylococcus aureus activity of silver oxide (Ag2O) nanoparticles synthesized from Aspergillus terreus VIT 2013. Production of Ag2O nanoparticles was confirmed by color change of fungal filtrate and UV light absorption at 450 nm. X-ray diffraction pattern showed 2θ values at 27, 32, 38 and 57°, which corresponded to the cubic structure of Ag2O nanocrystals. Fourier transform infrared spectroscopy indicated the presence of primary amine, carbonyl group, NO2 and silver, revealing protein mediated nanoparticle production. The scanning electron microscope image showed freely dispersed Ag2O nanoparticles. The nanoparticles were active against all methicillin resistant isolates and hence can be used as antibacterial agents against drug resistant bacteria.

No MeSH data available.


Related in: MedlinePlus