Hepatitis C virus attenuates mitochondrial lipid β-oxidation by downregulating mitochondrial trifunctional-protein expression.
Bottom Line: However, little is known about the effects of HCV on lipid β-oxidation.Here we show that in HCV-infected Huh7.5 cells, lipid β-oxidation was significantly attenuated.This study revealed that HCV impairs mitochondrial lipid β-oxidation, which results in low lipid combustion.
Affiliation: School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom email@example.com firstname.lastname@example.org.Show MeSH
Related in: MedlinePlus
Mentions: Plasmids pISRE-Luc and pRL-TK (20:1, wt/wt, ratio) were transfected into Huh7.5 cells on a 12-well plate with Fugene 6 transfection reagent. Transfected cells were split into a 96-well plate at 24 h posttransfection. Cells were further incubated for 48 h, and during the last 5 h, they were exposed to IFN-α at the concentrations indicated in Fig. 6. Measurement of luciferase activity was performed using the dual-luciferase reporter assay system for pISRE-Luc and pRL-TK.
Affiliation: School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom email@example.com firstname.lastname@example.org.