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An Anticancer Role of Hydrogen Sulfide in Human Gastric Cancer Cells.

Zhang L, Qi Q, Yang J, Sun D, Li C, Xue Y, Jiang Q, Tian Y, Xu C, Wang R - Oxid Med Cell Longev (2015)

Bottom Line: Hydrogen sulfide (H2S) can be synthesized in mammalian cells by cystathionine γ-lyase (CSE) and/or cystathionine β-synthase (CBS).We found that both CSE and CBS proteins were expressed in human gastric cancer cells and upregulated in human gastric carcinoma mucosa compared with those in noncancerous gastric samples.NaHS induced apoptosis of gastric cancer cells by regulating apoptosis related proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathophysiology, Harbin Medical University, Harbin 150081, China.

ABSTRACT
Hydrogen sulfide (H2S) can be synthesized in mammalian cells by cystathionine γ-lyase (CSE) and/or cystathionine β-synthase (CBS). Both CSE and CBS are expressed in rat gastric tissues but their role in human gastric neoplasia has been unclear. The aims of the present study were to detect CSE and CBS proteins in human gastric cancer and determine the effect of exogenous NaHS on the proliferation of gastric cancer cells. We found that both CSE and CBS proteins were expressed in human gastric cancer cells and upregulated in human gastric carcinoma mucosa compared with those in noncancerous gastric samples. NaHS induced apoptosis of gastric cancer cells by regulating apoptosis related proteins. Also, NaHS inhibited cancer cell migration and invasion. An antigastric cancer role of H2S is thus indicated.

No MeSH data available.


Related in: MedlinePlus

NaHS reduces cancer cell migration. Gastric cancer cells, SGC7901, were cultured in the absence or presence of NaHS. The effects of NaHS on cell migration were determined by a scratch assay. *P < 0.05 versus control. n = 3.
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fig6: NaHS reduces cancer cell migration. Gastric cancer cells, SGC7901, were cultured in the absence or presence of NaHS. The effects of NaHS on cell migration were determined by a scratch assay. *P < 0.05 versus control. n = 3.

Mentions: We further examined the effect of NaHS on SGC7901 cell migration. As shown in Figure 6, 0.8 mM NaHS significantly reduced cell migration in a scratch assay. NaHS-induced delay of coverage of the scratched area by cell migration is unlikely due to the reduced cell proliferation because the assay was carried out in presence of 0.1% serum to essentially stop cell proliferation. To evaluate the contribution of H2S on cell invasion, we added NaHS to the upper inserts of Boyden Chambers. As shown in Figure 7, 0.8 mM NaHS inhibited cancer cell invasion. To further determine the mechanisms of involvement in cell invasion, we tested MMP-2 and MMP-9 expression during NaHS treatment. As shown in Figure 8, 0.8 mM NaHS significantly attenuated MMP-2 expression, but there was no significant effect of NaHS observed on MMP-9 level.


An Anticancer Role of Hydrogen Sulfide in Human Gastric Cancer Cells.

Zhang L, Qi Q, Yang J, Sun D, Li C, Xue Y, Jiang Q, Tian Y, Xu C, Wang R - Oxid Med Cell Longev (2015)

NaHS reduces cancer cell migration. Gastric cancer cells, SGC7901, were cultured in the absence or presence of NaHS. The effects of NaHS on cell migration were determined by a scratch assay. *P < 0.05 versus control. n = 3.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4442311&req=5

fig6: NaHS reduces cancer cell migration. Gastric cancer cells, SGC7901, were cultured in the absence or presence of NaHS. The effects of NaHS on cell migration were determined by a scratch assay. *P < 0.05 versus control. n = 3.
Mentions: We further examined the effect of NaHS on SGC7901 cell migration. As shown in Figure 6, 0.8 mM NaHS significantly reduced cell migration in a scratch assay. NaHS-induced delay of coverage of the scratched area by cell migration is unlikely due to the reduced cell proliferation because the assay was carried out in presence of 0.1% serum to essentially stop cell proliferation. To evaluate the contribution of H2S on cell invasion, we added NaHS to the upper inserts of Boyden Chambers. As shown in Figure 7, 0.8 mM NaHS inhibited cancer cell invasion. To further determine the mechanisms of involvement in cell invasion, we tested MMP-2 and MMP-9 expression during NaHS treatment. As shown in Figure 8, 0.8 mM NaHS significantly attenuated MMP-2 expression, but there was no significant effect of NaHS observed on MMP-9 level.

Bottom Line: Hydrogen sulfide (H2S) can be synthesized in mammalian cells by cystathionine γ-lyase (CSE) and/or cystathionine β-synthase (CBS).We found that both CSE and CBS proteins were expressed in human gastric cancer cells and upregulated in human gastric carcinoma mucosa compared with those in noncancerous gastric samples.NaHS induced apoptosis of gastric cancer cells by regulating apoptosis related proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathophysiology, Harbin Medical University, Harbin 150081, China.

ABSTRACT
Hydrogen sulfide (H2S) can be synthesized in mammalian cells by cystathionine γ-lyase (CSE) and/or cystathionine β-synthase (CBS). Both CSE and CBS are expressed in rat gastric tissues but their role in human gastric neoplasia has been unclear. The aims of the present study were to detect CSE and CBS proteins in human gastric cancer and determine the effect of exogenous NaHS on the proliferation of gastric cancer cells. We found that both CSE and CBS proteins were expressed in human gastric cancer cells and upregulated in human gastric carcinoma mucosa compared with those in noncancerous gastric samples. NaHS induced apoptosis of gastric cancer cells by regulating apoptosis related proteins. Also, NaHS inhibited cancer cell migration and invasion. An antigastric cancer role of H2S is thus indicated.

No MeSH data available.


Related in: MedlinePlus