Limits...
An Anticancer Role of Hydrogen Sulfide in Human Gastric Cancer Cells.

Zhang L, Qi Q, Yang J, Sun D, Li C, Xue Y, Jiang Q, Tian Y, Xu C, Wang R - Oxid Med Cell Longev (2015)

Bottom Line: Hydrogen sulfide (H2S) can be synthesized in mammalian cells by cystathionine γ-lyase (CSE) and/or cystathionine β-synthase (CBS).We found that both CSE and CBS proteins were expressed in human gastric cancer cells and upregulated in human gastric carcinoma mucosa compared with those in noncancerous gastric samples.NaHS induced apoptosis of gastric cancer cells by regulating apoptosis related proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathophysiology, Harbin Medical University, Harbin 150081, China.

ABSTRACT
Hydrogen sulfide (H2S) can be synthesized in mammalian cells by cystathionine γ-lyase (CSE) and/or cystathionine β-synthase (CBS). Both CSE and CBS are expressed in rat gastric tissues but their role in human gastric neoplasia has been unclear. The aims of the present study were to detect CSE and CBS proteins in human gastric cancer and determine the effect of exogenous NaHS on the proliferation of gastric cancer cells. We found that both CSE and CBS proteins were expressed in human gastric cancer cells and upregulated in human gastric carcinoma mucosa compared with those in noncancerous gastric samples. NaHS induced apoptosis of gastric cancer cells by regulating apoptosis related proteins. Also, NaHS inhibited cancer cell migration and invasion. An antigastric cancer role of H2S is thus indicated.

No MeSH data available.


Related in: MedlinePlus

NaHS increased the levels of Bax, caspase 3, and Cyt C in SGC7901cells, detected by western blotting.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4442311&req=5

fig4: NaHS increased the levels of Bax, caspase 3, and Cyt C in SGC7901cells, detected by western blotting.

Mentions: To investigate whether H2S is involved in apoptosis, we performed apoptosis test using Hoechst-Propidium Iodide staining of cells with different treatments. As shown in Figure 3, NaHS treatment enhanced apoptotic rate of cells. PPG increased mitotic rate. The levels of apoptosis-related proteins, Bax, Cyt C, and caspase 3 were increased after NaHS treatment (Figure 4). We next sought to reveal the role of NaHS on the expression of cell cycle proteins. Cyclin D1 was upregulated during 0.5 h, 2 h, and 8 h, but downregulated at 12 h of NaHS treatment. On the other hand, cell cycle inhibitors p21waf1/cip1 and p27kip1 were downregulated by NaHS in a time-dependent manner (Figure 5).


An Anticancer Role of Hydrogen Sulfide in Human Gastric Cancer Cells.

Zhang L, Qi Q, Yang J, Sun D, Li C, Xue Y, Jiang Q, Tian Y, Xu C, Wang R - Oxid Med Cell Longev (2015)

NaHS increased the levels of Bax, caspase 3, and Cyt C in SGC7901cells, detected by western blotting.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4442311&req=5

fig4: NaHS increased the levels of Bax, caspase 3, and Cyt C in SGC7901cells, detected by western blotting.
Mentions: To investigate whether H2S is involved in apoptosis, we performed apoptosis test using Hoechst-Propidium Iodide staining of cells with different treatments. As shown in Figure 3, NaHS treatment enhanced apoptotic rate of cells. PPG increased mitotic rate. The levels of apoptosis-related proteins, Bax, Cyt C, and caspase 3 were increased after NaHS treatment (Figure 4). We next sought to reveal the role of NaHS on the expression of cell cycle proteins. Cyclin D1 was upregulated during 0.5 h, 2 h, and 8 h, but downregulated at 12 h of NaHS treatment. On the other hand, cell cycle inhibitors p21waf1/cip1 and p27kip1 were downregulated by NaHS in a time-dependent manner (Figure 5).

Bottom Line: Hydrogen sulfide (H2S) can be synthesized in mammalian cells by cystathionine γ-lyase (CSE) and/or cystathionine β-synthase (CBS).We found that both CSE and CBS proteins were expressed in human gastric cancer cells and upregulated in human gastric carcinoma mucosa compared with those in noncancerous gastric samples.NaHS induced apoptosis of gastric cancer cells by regulating apoptosis related proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathophysiology, Harbin Medical University, Harbin 150081, China.

ABSTRACT
Hydrogen sulfide (H2S) can be synthesized in mammalian cells by cystathionine γ-lyase (CSE) and/or cystathionine β-synthase (CBS). Both CSE and CBS are expressed in rat gastric tissues but their role in human gastric neoplasia has been unclear. The aims of the present study were to detect CSE and CBS proteins in human gastric cancer and determine the effect of exogenous NaHS on the proliferation of gastric cancer cells. We found that both CSE and CBS proteins were expressed in human gastric cancer cells and upregulated in human gastric carcinoma mucosa compared with those in noncancerous gastric samples. NaHS induced apoptosis of gastric cancer cells by regulating apoptosis related proteins. Also, NaHS inhibited cancer cell migration and invasion. An antigastric cancer role of H2S is thus indicated.

No MeSH data available.


Related in: MedlinePlus