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Reinforcing Lipid A Acylation on the Cell Surface of Acinetobacter baumannii Promotes Cationic Antimicrobial Peptide Resistance and Desiccation Survival.

Boll JM, Tucker AT, Klein DR, Beltran AM, Brodbelt JS, Davies BW, Trent MS - MBio (2015)

Bottom Line: Acinetobacter baumannii is an emerging Gram-negative pathogen found in hospitals and intensive care units.Whereas in Escherichia coli and Salmonella, increased production of the outer membrane acyltransferase PagP results in formation of protective hepta-acylated lipid A, which reinforces the lipopolysaccharide portion of the outer membrane barrier, A. baumannii does not carry a gene that encodes a PagP homolog.Emergence of multidrug- and extremely drug-resistant A. baumannii illustrates the ineffectiveness of current prevention and treatment options.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas, USA.

No MeSH data available.


Related in: MedlinePlus

Galleria mellonella virulence model. G. mellonella survival is dependent on LpxMAb. G. mellonella larvae were injected with approximately 1 × 106 CFU and incubated at 37°C. Killing was monitored every 24 h for 6 days.
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fig7: Galleria mellonella virulence model. G. mellonella survival is dependent on LpxMAb. G. mellonella larvae were injected with approximately 1 × 106 CFU and incubated at 37°C. Killing was monitored every 24 h for 6 days.

Mentions: To determine the susceptibility of killing in the A. baumannii acyltransferase mutants, we injected equivalent numbers of CFU from wild-type, mutant, and complemented strains into G. mellonella and monitored bacterial killing. After 72 h, all wax moth larvae injected with the wild-type and complemented A. baumannii strains died, whereas all worms that were challenged with phosphate-buffered saline (PBS) and the A. baumannii ΔlpxMAb strain survived. In contrast, only one of the larvae injected with the A. baumannii LpxLAb mutant survived (Fig. 7). These data suggest that LpxMAb-dependent lipid A acylation is important for A. baumannii survival and virulence in invertebrates.


Reinforcing Lipid A Acylation on the Cell Surface of Acinetobacter baumannii Promotes Cationic Antimicrobial Peptide Resistance and Desiccation Survival.

Boll JM, Tucker AT, Klein DR, Beltran AM, Brodbelt JS, Davies BW, Trent MS - MBio (2015)

Galleria mellonella virulence model. G. mellonella survival is dependent on LpxMAb. G. mellonella larvae were injected with approximately 1 × 106 CFU and incubated at 37°C. Killing was monitored every 24 h for 6 days.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4442142&req=5

fig7: Galleria mellonella virulence model. G. mellonella survival is dependent on LpxMAb. G. mellonella larvae were injected with approximately 1 × 106 CFU and incubated at 37°C. Killing was monitored every 24 h for 6 days.
Mentions: To determine the susceptibility of killing in the A. baumannii acyltransferase mutants, we injected equivalent numbers of CFU from wild-type, mutant, and complemented strains into G. mellonella and monitored bacterial killing. After 72 h, all wax moth larvae injected with the wild-type and complemented A. baumannii strains died, whereas all worms that were challenged with phosphate-buffered saline (PBS) and the A. baumannii ΔlpxMAb strain survived. In contrast, only one of the larvae injected with the A. baumannii LpxLAb mutant survived (Fig. 7). These data suggest that LpxMAb-dependent lipid A acylation is important for A. baumannii survival and virulence in invertebrates.

Bottom Line: Acinetobacter baumannii is an emerging Gram-negative pathogen found in hospitals and intensive care units.Whereas in Escherichia coli and Salmonella, increased production of the outer membrane acyltransferase PagP results in formation of protective hepta-acylated lipid A, which reinforces the lipopolysaccharide portion of the outer membrane barrier, A. baumannii does not carry a gene that encodes a PagP homolog.Emergence of multidrug- and extremely drug-resistant A. baumannii illustrates the ineffectiveness of current prevention and treatment options.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biosciences, University of Texas at Austin, Austin, Texas, USA.

No MeSH data available.


Related in: MedlinePlus