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An in-vitro-in-vivo model for the transdermal delivery of cholecalciferol for the purposes of rodent management.

Davies J, Ingham A - Int J Pharm (2015)

Bottom Line: The natural selection of anticoagulant resistant rats has resulted in a need for an alternative to anticoagulant rodenticides which differs in both active ingredient and in the method of dosing.A 1 ml dose of 50/50 (v/v) DMSO/ethanol containing 15% (v/v) PEG 200 and 20% (w/v) cholecalciferol was judged as 'sufficiently effective' in line with the European Union's Biocidal Products Regulation (No. 528/2012) during in-vivo studies.This dose was found to cause 100% mortality in a rat population in 64.4h (± 22h).

View Article: PubMed Central - PubMed

Affiliation: School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK.

No MeSH data available.


Related in: MedlinePlus

Cholecalciferol permeation profiles obtained with the cellulose tubing in-vitro model. All formulations contained 10% (w/v) cholecalciferol released from a volume of 1 ml. Mean values ± SD (n = 3).
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fig0005: Cholecalciferol permeation profiles obtained with the cellulose tubing in-vitro model. All formulations contained 10% (w/v) cholecalciferol released from a volume of 1 ml. Mean values ± SD (n = 3).

Mentions: Cholecalciferol permeation profiles using: DMSO, ethanol, oleic acid, 2-pyrrolidone and water as penetration enhancers were obtained through cellulose tubing in an in-vitro model and are illustrated in Fig. 1. Fig. 2 shows the drug flux comparisons between the formulations tested. The 90/10 (v/v) DMSO/ethanol (0.26 ± 0.044 mg/cm2 h) formulation was shown to significantly increase (p < 0.1) the drug flux of cholecalciferol when compared to the ethanol (0.14 ± 0.008 mg/cm2 h) and 2-pyrrolidone (0.13 ± 0.038 mg/cm2 h) formulations. Based on these results, the DMSO/ethanol co-solvent was selected for further investigations as a penetration enhancer for cholecalciferol.


An in-vitro-in-vivo model for the transdermal delivery of cholecalciferol for the purposes of rodent management.

Davies J, Ingham A - Int J Pharm (2015)

Cholecalciferol permeation profiles obtained with the cellulose tubing in-vitro model. All formulations contained 10% (w/v) cholecalciferol released from a volume of 1 ml. Mean values ± SD (n = 3).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4441109&req=5

fig0005: Cholecalciferol permeation profiles obtained with the cellulose tubing in-vitro model. All formulations contained 10% (w/v) cholecalciferol released from a volume of 1 ml. Mean values ± SD (n = 3).
Mentions: Cholecalciferol permeation profiles using: DMSO, ethanol, oleic acid, 2-pyrrolidone and water as penetration enhancers were obtained through cellulose tubing in an in-vitro model and are illustrated in Fig. 1. Fig. 2 shows the drug flux comparisons between the formulations tested. The 90/10 (v/v) DMSO/ethanol (0.26 ± 0.044 mg/cm2 h) formulation was shown to significantly increase (p < 0.1) the drug flux of cholecalciferol when compared to the ethanol (0.14 ± 0.008 mg/cm2 h) and 2-pyrrolidone (0.13 ± 0.038 mg/cm2 h) formulations. Based on these results, the DMSO/ethanol co-solvent was selected for further investigations as a penetration enhancer for cholecalciferol.

Bottom Line: The natural selection of anticoagulant resistant rats has resulted in a need for an alternative to anticoagulant rodenticides which differs in both active ingredient and in the method of dosing.A 1 ml dose of 50/50 (v/v) DMSO/ethanol containing 15% (v/v) PEG 200 and 20% (w/v) cholecalciferol was judged as 'sufficiently effective' in line with the European Union's Biocidal Products Regulation (No. 528/2012) during in-vivo studies.This dose was found to cause 100% mortality in a rat population in 64.4h (± 22h).

View Article: PubMed Central - PubMed

Affiliation: School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, UK.

No MeSH data available.


Related in: MedlinePlus