Limits...
Delayed reconstitution of B cell immunity to pneumococcus in HIV-infected Malawian children on antiretroviral therapy.

Iwajomo OH, Moons P, Nkhata R, Mzinza D, Ogunniyi AD, Williams NA, Heyderman RS, Finn A - J. Infect. (2014)

Bottom Line: The proportions of mature naïve B cells (CD19(+) CD10(-) CD27(-) CD21(hi)) and resting memory B cells (CD19(+) CD27(+) CD21(hi)) increased and apoptosis-prone mature activated B cells (CD19(+) CD21(lo) CD10(-)) decreased markedly by 12 months.These data show that, in chronically HIV-infected children receiving ART, improvement in B-cell memory profiles and function is slower than CD4(+) T-cells.This supports early initiation of ART and informs research into optimal timing of immunization with pneumococcal vaccines.

View Article: PubMed Central - PubMed

Affiliation: School of Cellular and Molecular Medicine, University of Bristol, Bristol, United Kingdom; Malawi Liverpool Wellcome Trust Clinical Research Programme, University of Malawi College of Medicine, Blantyre, Malawi.

No MeSH data available.


Related in: MedlinePlus

Increase in mature naïve & resting memory B cells and reduction of apoptosis prone mature activated B cells in HIV-infected children following initiation of antiretroviral therapy (ART). Percentages of (A) mature naïve B cells (CD19+ CD10− CD27− CD21hi) (B) resting memory B cells (CD19+ CD27+ CD21hi) (C) mature activated B cells (CD19+ CD21lo CD10−) (D) immature transitional B cells (CD19+ CD10+ CD27−) measured using flow cytometry in HIV-infected children on ART over 12 months. Horizontal bars in A–D represent median values. The broken horizontal lines in A–D represent median values in HIV-uninfected controls recruited as part of separate contemporaneous study reported elsewhere and interquartile range for these controls have been incorporated in the y-axis.10 Comparisons were made using Friedman's test to evaluate the effect of ART across all time points.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4441108&req=5

fig2: Increase in mature naïve & resting memory B cells and reduction of apoptosis prone mature activated B cells in HIV-infected children following initiation of antiretroviral therapy (ART). Percentages of (A) mature naïve B cells (CD19+ CD10− CD27− CD21hi) (B) resting memory B cells (CD19+ CD27+ CD21hi) (C) mature activated B cells (CD19+ CD21lo CD10−) (D) immature transitional B cells (CD19+ CD10+ CD27−) measured using flow cytometry in HIV-infected children on ART over 12 months. Horizontal bars in A–D represent median values. The broken horizontal lines in A–D represent median values in HIV-uninfected controls recruited as part of separate contemporaneous study reported elsewhere and interquartile range for these controls have been incorporated in the y-axis.10 Comparisons were made using Friedman's test to evaluate the effect of ART across all time points.

Mentions: Extending our recent report of an apoptosis-prone phenotype in HIV-infected children,10 we measured trends in circulating B cell subsets during 12 months' ART and observed increases in proportions of both mature naïve (CD19+ CD10− CD27− CD21hi) and resting memory B cells (CD19+ CD27+ CD21hi) (P < 0.0001, P = 0.04) which occurred largely over the first 3 months and to levels, in the former subset, that were higher than those seen in uninfected controls while in the latter they remained below normal median values (Fig. 2A–B). There were corresponding falls in proportions of apoptosis-prone mature activated (CD19+ CD21lo CD10−) B cells (P < 0.0001) to levels seen in uninfected controls (Fig. 2C). However, no significant or consistent trends in numbers of apoptosis-prone immature transitional (CD19+ CD10+ CD27−) B cell percentages were observed (Fig. 2D).


Delayed reconstitution of B cell immunity to pneumococcus in HIV-infected Malawian children on antiretroviral therapy.

Iwajomo OH, Moons P, Nkhata R, Mzinza D, Ogunniyi AD, Williams NA, Heyderman RS, Finn A - J. Infect. (2014)

Increase in mature naïve & resting memory B cells and reduction of apoptosis prone mature activated B cells in HIV-infected children following initiation of antiretroviral therapy (ART). Percentages of (A) mature naïve B cells (CD19+ CD10− CD27− CD21hi) (B) resting memory B cells (CD19+ CD27+ CD21hi) (C) mature activated B cells (CD19+ CD21lo CD10−) (D) immature transitional B cells (CD19+ CD10+ CD27−) measured using flow cytometry in HIV-infected children on ART over 12 months. Horizontal bars in A–D represent median values. The broken horizontal lines in A–D represent median values in HIV-uninfected controls recruited as part of separate contemporaneous study reported elsewhere and interquartile range for these controls have been incorporated in the y-axis.10 Comparisons were made using Friedman's test to evaluate the effect of ART across all time points.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4441108&req=5

fig2: Increase in mature naïve & resting memory B cells and reduction of apoptosis prone mature activated B cells in HIV-infected children following initiation of antiretroviral therapy (ART). Percentages of (A) mature naïve B cells (CD19+ CD10− CD27− CD21hi) (B) resting memory B cells (CD19+ CD27+ CD21hi) (C) mature activated B cells (CD19+ CD21lo CD10−) (D) immature transitional B cells (CD19+ CD10+ CD27−) measured using flow cytometry in HIV-infected children on ART over 12 months. Horizontal bars in A–D represent median values. The broken horizontal lines in A–D represent median values in HIV-uninfected controls recruited as part of separate contemporaneous study reported elsewhere and interquartile range for these controls have been incorporated in the y-axis.10 Comparisons were made using Friedman's test to evaluate the effect of ART across all time points.
Mentions: Extending our recent report of an apoptosis-prone phenotype in HIV-infected children,10 we measured trends in circulating B cell subsets during 12 months' ART and observed increases in proportions of both mature naïve (CD19+ CD10− CD27− CD21hi) and resting memory B cells (CD19+ CD27+ CD21hi) (P < 0.0001, P = 0.04) which occurred largely over the first 3 months and to levels, in the former subset, that were higher than those seen in uninfected controls while in the latter they remained below normal median values (Fig. 2A–B). There were corresponding falls in proportions of apoptosis-prone mature activated (CD19+ CD21lo CD10−) B cells (P < 0.0001) to levels seen in uninfected controls (Fig. 2C). However, no significant or consistent trends in numbers of apoptosis-prone immature transitional (CD19+ CD10+ CD27−) B cell percentages were observed (Fig. 2D).

Bottom Line: The proportions of mature naïve B cells (CD19(+) CD10(-) CD27(-) CD21(hi)) and resting memory B cells (CD19(+) CD27(+) CD21(hi)) increased and apoptosis-prone mature activated B cells (CD19(+) CD21(lo) CD10(-)) decreased markedly by 12 months.These data show that, in chronically HIV-infected children receiving ART, improvement in B-cell memory profiles and function is slower than CD4(+) T-cells.This supports early initiation of ART and informs research into optimal timing of immunization with pneumococcal vaccines.

View Article: PubMed Central - PubMed

Affiliation: School of Cellular and Molecular Medicine, University of Bristol, Bristol, United Kingdom; Malawi Liverpool Wellcome Trust Clinical Research Programme, University of Malawi College of Medicine, Blantyre, Malawi.

No MeSH data available.


Related in: MedlinePlus