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Delayed reconstitution of B cell immunity to pneumococcus in HIV-infected Malawian children on antiretroviral therapy.

Iwajomo OH, Moons P, Nkhata R, Mzinza D, Ogunniyi AD, Williams NA, Heyderman RS, Finn A - J. Infect. (2014)

Bottom Line: The proportions of mature naïve B cells (CD19(+) CD10(-) CD27(-) CD21(hi)) and resting memory B cells (CD19(+) CD27(+) CD21(hi)) increased and apoptosis-prone mature activated B cells (CD19(+) CD21(lo) CD10(-)) decreased markedly by 12 months.These data show that, in chronically HIV-infected children receiving ART, improvement in B-cell memory profiles and function is slower than CD4(+) T-cells.This supports early initiation of ART and informs research into optimal timing of immunization with pneumococcal vaccines.

View Article: PubMed Central - PubMed

Affiliation: School of Cellular and Molecular Medicine, University of Bristol, Bristol, United Kingdom; Malawi Liverpool Wellcome Trust Clinical Research Programme, University of Malawi College of Medicine, Blantyre, Malawi.

No MeSH data available.


Related in: MedlinePlus

Progressive changes in major lymphocyte subsets of HIV-infected Malawian children over the course of 12 months' antiretroviral therapy (ART). Absolute numbers of circulating (A) CD4+ T cells (B) CD8+ T cells and (C) CD19+ B cells; proportions of (D) CD4+ T cells (E) CD8+ T cells and (F) CD19+ B cells measured using flow cytometry. Horizontal bars in A–F represent median values. The broken horizontal lines in A–F represent median values in HIV-uninfected controls recruited as part of separate contemporaneous study reported elsewhere and interquartile range for these controls have been incorporated in the y-axis.10 Comparisons were made using Friedman's test to evaluate the effect of ART across all time points.
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fig1: Progressive changes in major lymphocyte subsets of HIV-infected Malawian children over the course of 12 months' antiretroviral therapy (ART). Absolute numbers of circulating (A) CD4+ T cells (B) CD8+ T cells and (C) CD19+ B cells; proportions of (D) CD4+ T cells (E) CD8+ T cells and (F) CD19+ B cells measured using flow cytometry. Horizontal bars in A–F represent median values. The broken horizontal lines in A–F represent median values in HIV-uninfected controls recruited as part of separate contemporaneous study reported elsewhere and interquartile range for these controls have been incorporated in the y-axis.10 Comparisons were made using Friedman's test to evaluate the effect of ART across all time points.

Mentions: As expected, both absolute and percentage CD4+ T cell counts rose significantly (P < 0.0001) following initiation of ART over the 12 months of the study. While on average, rises in absolute counts were most obvious during the first 3 months, rises in percentages were more progressive over the whole observation period although in neither case did they reach median values seen in HIV-uninfected controls (Fig. 1A and D). In contrast, no statistically significant trends in absolute CD8+ T cell and CD19+ B cell counts were seen over the same period (Fig. 1B and C). Values for CD8+ T cells remained above those seen in uninfected controls showing some apparent trend towards these normal values (Fig. 1B and E) but median CD19+ B cell values remained consistently lower than control values (Fig. 1C and F).


Delayed reconstitution of B cell immunity to pneumococcus in HIV-infected Malawian children on antiretroviral therapy.

Iwajomo OH, Moons P, Nkhata R, Mzinza D, Ogunniyi AD, Williams NA, Heyderman RS, Finn A - J. Infect. (2014)

Progressive changes in major lymphocyte subsets of HIV-infected Malawian children over the course of 12 months' antiretroviral therapy (ART). Absolute numbers of circulating (A) CD4+ T cells (B) CD8+ T cells and (C) CD19+ B cells; proportions of (D) CD4+ T cells (E) CD8+ T cells and (F) CD19+ B cells measured using flow cytometry. Horizontal bars in A–F represent median values. The broken horizontal lines in A–F represent median values in HIV-uninfected controls recruited as part of separate contemporaneous study reported elsewhere and interquartile range for these controls have been incorporated in the y-axis.10 Comparisons were made using Friedman's test to evaluate the effect of ART across all time points.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4441108&req=5

fig1: Progressive changes in major lymphocyte subsets of HIV-infected Malawian children over the course of 12 months' antiretroviral therapy (ART). Absolute numbers of circulating (A) CD4+ T cells (B) CD8+ T cells and (C) CD19+ B cells; proportions of (D) CD4+ T cells (E) CD8+ T cells and (F) CD19+ B cells measured using flow cytometry. Horizontal bars in A–F represent median values. The broken horizontal lines in A–F represent median values in HIV-uninfected controls recruited as part of separate contemporaneous study reported elsewhere and interquartile range for these controls have been incorporated in the y-axis.10 Comparisons were made using Friedman's test to evaluate the effect of ART across all time points.
Mentions: As expected, both absolute and percentage CD4+ T cell counts rose significantly (P < 0.0001) following initiation of ART over the 12 months of the study. While on average, rises in absolute counts were most obvious during the first 3 months, rises in percentages were more progressive over the whole observation period although in neither case did they reach median values seen in HIV-uninfected controls (Fig. 1A and D). In contrast, no statistically significant trends in absolute CD8+ T cell and CD19+ B cell counts were seen over the same period (Fig. 1B and C). Values for CD8+ T cells remained above those seen in uninfected controls showing some apparent trend towards these normal values (Fig. 1B and E) but median CD19+ B cell values remained consistently lower than control values (Fig. 1C and F).

Bottom Line: The proportions of mature naïve B cells (CD19(+) CD10(-) CD27(-) CD21(hi)) and resting memory B cells (CD19(+) CD27(+) CD21(hi)) increased and apoptosis-prone mature activated B cells (CD19(+) CD21(lo) CD10(-)) decreased markedly by 12 months.These data show that, in chronically HIV-infected children receiving ART, improvement in B-cell memory profiles and function is slower than CD4(+) T-cells.This supports early initiation of ART and informs research into optimal timing of immunization with pneumococcal vaccines.

View Article: PubMed Central - PubMed

Affiliation: School of Cellular and Molecular Medicine, University of Bristol, Bristol, United Kingdom; Malawi Liverpool Wellcome Trust Clinical Research Programme, University of Malawi College of Medicine, Blantyre, Malawi.

No MeSH data available.


Related in: MedlinePlus