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Swirl Flow Bioreactor coupled with Cu-alginate beads: A system for the eradication of Coliform and Escherichia coli from biological effluents.

Atkinson S, Thomas SF, Goddard P, Bransgrove RM, Mason PT, Oak A, Bansode A, Patankar R, Gleason ZD, Sim MK, Whitesell A, Allen MJ - Sci Rep (2015)

Bottom Line: It is estimated that approximately 1.1 billion people globally drink unsafe water.We previously reported both a novel copper-alginate bead, which quickly reduces pathogen loading in waste streams and the incorporation of these beads into a novel swirl flow bioreactor (SFB), of low capital and running costs and of simple construction from commercially available plumbing pipes and fittings.However, the system was slightly less effective in reducing E. coli viability, with a MLRV of 1.80.

View Article: PubMed Central - PubMed

Affiliation: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, UK.

ABSTRACT
It is estimated that approximately 1.1 billion people globally drink unsafe water. We previously reported both a novel copper-alginate bead, which quickly reduces pathogen loading in waste streams and the incorporation of these beads into a novel swirl flow bioreactor (SFB), of low capital and running costs and of simple construction from commercially available plumbing pipes and fittings. The purpose of the present study was to trial this system for pathogen reduction in waste streams from an operating Dewats system in Hinjewadi, Pune, India and in both simulated and real waste streams in Seattle, Washington, USA. The trials in India, showed a complete inactivation of coliforms in the discharged effluent (Mean Log removal Value (MLRV) = 3.51), accompanied by a total inactivation of E. coli with a MLRV of 1.95. The secondary clarifier effluent also showed a 4.38 MLRV in viable coliforms during treatment. However, the system was slightly less effective in reducing E. coli viability, with a MLRV of 1.80. The trials in Seattle also demonstrated the efficacy of the system in the reduction of viable bacteria, with a LRV of 5.67 observed of viable Raoultella terrigena cells (100%).

No MeSH data available.


Related in: MedlinePlus

The turbulence and swirl characteristics of the swirl flow bioreactor, A) without beads and B) with beads.
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f3: The turbulence and swirl characteristics of the swirl flow bioreactor, A) without beads and B) with beads.

Mentions: Each effluent (~6.5 L) was decanted into the Swirl Flow Bioreactor (see Figure 2 and further detailed description of the Swirl Flow Bioreactor can be seen in Thomas et al17) and the bioreactor started without the presence of the Cu-Alginate composite beads (see Figure 3a). Triplicate samples were taken for microbial assay at 0, 10, 20 and 30 mins. Total ATP, E. coli and Coliform assay analyses were completed using fluorescence-based enzyme assays (Hygiena, Hertfordshire, UK), according to the manufacture's instruction and a handheld Luminometer (Model EnSURE, Hygiena, Hertfordshire, UK). The raw Relative Light Unit (RLU) readings from the commercialised detection kit were converted to CFU/mL according to the manufacturer's formula. The Swirl Flow Bioreactor was rinsed repeatedly with clean water after use.


Swirl Flow Bioreactor coupled with Cu-alginate beads: A system for the eradication of Coliform and Escherichia coli from biological effluents.

Atkinson S, Thomas SF, Goddard P, Bransgrove RM, Mason PT, Oak A, Bansode A, Patankar R, Gleason ZD, Sim MK, Whitesell A, Allen MJ - Sci Rep (2015)

The turbulence and swirl characteristics of the swirl flow bioreactor, A) without beads and B) with beads.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440980&req=5

f3: The turbulence and swirl characteristics of the swirl flow bioreactor, A) without beads and B) with beads.
Mentions: Each effluent (~6.5 L) was decanted into the Swirl Flow Bioreactor (see Figure 2 and further detailed description of the Swirl Flow Bioreactor can be seen in Thomas et al17) and the bioreactor started without the presence of the Cu-Alginate composite beads (see Figure 3a). Triplicate samples were taken for microbial assay at 0, 10, 20 and 30 mins. Total ATP, E. coli and Coliform assay analyses were completed using fluorescence-based enzyme assays (Hygiena, Hertfordshire, UK), according to the manufacture's instruction and a handheld Luminometer (Model EnSURE, Hygiena, Hertfordshire, UK). The raw Relative Light Unit (RLU) readings from the commercialised detection kit were converted to CFU/mL according to the manufacturer's formula. The Swirl Flow Bioreactor was rinsed repeatedly with clean water after use.

Bottom Line: It is estimated that approximately 1.1 billion people globally drink unsafe water.We previously reported both a novel copper-alginate bead, which quickly reduces pathogen loading in waste streams and the incorporation of these beads into a novel swirl flow bioreactor (SFB), of low capital and running costs and of simple construction from commercially available plumbing pipes and fittings.However, the system was slightly less effective in reducing E. coli viability, with a MLRV of 1.80.

View Article: PubMed Central - PubMed

Affiliation: Plymouth Marine Laboratory, Prospect Place, The Hoe, Plymouth, PL1 3DH, UK.

ABSTRACT
It is estimated that approximately 1.1 billion people globally drink unsafe water. We previously reported both a novel copper-alginate bead, which quickly reduces pathogen loading in waste streams and the incorporation of these beads into a novel swirl flow bioreactor (SFB), of low capital and running costs and of simple construction from commercially available plumbing pipes and fittings. The purpose of the present study was to trial this system for pathogen reduction in waste streams from an operating Dewats system in Hinjewadi, Pune, India and in both simulated and real waste streams in Seattle, Washington, USA. The trials in India, showed a complete inactivation of coliforms in the discharged effluent (Mean Log removal Value (MLRV) = 3.51), accompanied by a total inactivation of E. coli with a MLRV of 1.95. The secondary clarifier effluent also showed a 4.38 MLRV in viable coliforms during treatment. However, the system was slightly less effective in reducing E. coli viability, with a MLRV of 1.80. The trials in Seattle also demonstrated the efficacy of the system in the reduction of viable bacteria, with a LRV of 5.67 observed of viable Raoultella terrigena cells (100%).

No MeSH data available.


Related in: MedlinePlus