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Antibacterial activity of fresh pomegranate juice against clinical strains of Staphylococcus epidermidis.

Betanzos-Cabrera G, Montes-Rubio PY, Fabela-Illescas HE, Belefant-Miller H, Cancino-Diaz JC - Food Nutr Res (2015)

Bottom Line: Additionally, the antimicrobial activity of FPJ was compared against commercial beverages containing pomegranate: Ocean Spray(®) had a MIC100% at 20%, followed by Del Valle(®) with a MIC15% at 20% concentration only.The beverages Jumex(®) and Sonrisa(®) did not have any antimicrobial activity.Overall, FPJ had antimicrobial activity, which might be attributed to its high polyphenol content and antioxidant capacity.

View Article: PubMed Central - PubMed

Affiliation: Área Académica de Nutrición, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Hidalgo, Mexico; gbetanzo@uaeh.edu.mx.

ABSTRACT

Background: Polyphenols have received a great deal of attention due to their biological functions. Pomegranate (Punica granatum L.) is a polyphenol-rich fruit. In the past decade, studies testing the antimicrobial activity of pomegranates almost exclusively used solvent extracts instead of fresh pomegranate juice (FPJ). The use of FPJ instead of solvent extracts would reduce toxicity issues while increasing patient acceptance. We established a model to test FPJ as a natural antimicrobial agent.

Objective: To evaluate the antimicrobial activity of FPJ on clinical isolates of multidrug-resistant Staphylococcus epidermidis strains.

Design: Sixty strains of S. epidermidis isolated from ocular infections were grown in the presence of FPJ, and minimum inhibitory concentration (MIC) was determined by broth and agar dilution methods.

Results: FPJ at 20% had a MIC equal to 100% (MIC100%) on all 60 strains tested. This inhibition of FPJ was confirmed by the growth kinetics of a multidrug-resistant strain exposed to different concentrations of FPJ. Additionally, the antimicrobial activity of FPJ was compared against commercial beverages containing pomegranate: Ocean Spray(®) had a MIC100% at 20%, followed by Del Valle(®) with a MIC15% at 20% concentration only. The beverages Jumex(®) and Sonrisa(®) did not have any antimicrobial activity. FPJ had the highest polyphenol content and antioxidant capacity.

Conclusions: Overall, FPJ had antimicrobial activity, which might be attributed to its high polyphenol content and antioxidant capacity.

No MeSH data available.


Related in: MedlinePlus

Total polyphenolics as gallic acid equivalents (GAE). Total polyphenol content was determined by the Folin-Ciocalteu method for all beverages. Fresh PJ – fresh pomegranate juice. An asterisk (*) indicates a significant difference (p<0.05) between FPJ and that beverage, according to a one-way ANOVA with a Tukey test.
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Figure 0005: Total polyphenolics as gallic acid equivalents (GAE). Total polyphenol content was determined by the Folin-Ciocalteu method for all beverages. Fresh PJ – fresh pomegranate juice. An asterisk (*) indicates a significant difference (p<0.05) between FPJ and that beverage, according to a one-way ANOVA with a Tukey test.

Mentions: The total polyphenol content of FPJ and pomegranate-based beverages occurred in the following order: FPJ>Ocean Spray®>Del Valle®>Jumex®=Sonrisa® (Fig. 5). FPJ also had the highest antioxidant capacity in the two assays employed (Fig. 6a and b). When the measurements for polyphenols and antioxidants were combined into a single index of antioxidant activity (Fig. 6c), the rank order was the same as for the antimicrobial activity (Fig. 2).


Antibacterial activity of fresh pomegranate juice against clinical strains of Staphylococcus epidermidis.

Betanzos-Cabrera G, Montes-Rubio PY, Fabela-Illescas HE, Belefant-Miller H, Cancino-Diaz JC - Food Nutr Res (2015)

Total polyphenolics as gallic acid equivalents (GAE). Total polyphenol content was determined by the Folin-Ciocalteu method for all beverages. Fresh PJ – fresh pomegranate juice. An asterisk (*) indicates a significant difference (p<0.05) between FPJ and that beverage, according to a one-way ANOVA with a Tukey test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440876&req=5

Figure 0005: Total polyphenolics as gallic acid equivalents (GAE). Total polyphenol content was determined by the Folin-Ciocalteu method for all beverages. Fresh PJ – fresh pomegranate juice. An asterisk (*) indicates a significant difference (p<0.05) between FPJ and that beverage, according to a one-way ANOVA with a Tukey test.
Mentions: The total polyphenol content of FPJ and pomegranate-based beverages occurred in the following order: FPJ>Ocean Spray®>Del Valle®>Jumex®=Sonrisa® (Fig. 5). FPJ also had the highest antioxidant capacity in the two assays employed (Fig. 6a and b). When the measurements for polyphenols and antioxidants were combined into a single index of antioxidant activity (Fig. 6c), the rank order was the same as for the antimicrobial activity (Fig. 2).

Bottom Line: Additionally, the antimicrobial activity of FPJ was compared against commercial beverages containing pomegranate: Ocean Spray(®) had a MIC100% at 20%, followed by Del Valle(®) with a MIC15% at 20% concentration only.The beverages Jumex(®) and Sonrisa(®) did not have any antimicrobial activity.Overall, FPJ had antimicrobial activity, which might be attributed to its high polyphenol content and antioxidant capacity.

View Article: PubMed Central - PubMed

Affiliation: Área Académica de Nutrición, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Hidalgo, Mexico; gbetanzo@uaeh.edu.mx.

ABSTRACT

Background: Polyphenols have received a great deal of attention due to their biological functions. Pomegranate (Punica granatum L.) is a polyphenol-rich fruit. In the past decade, studies testing the antimicrobial activity of pomegranates almost exclusively used solvent extracts instead of fresh pomegranate juice (FPJ). The use of FPJ instead of solvent extracts would reduce toxicity issues while increasing patient acceptance. We established a model to test FPJ as a natural antimicrobial agent.

Objective: To evaluate the antimicrobial activity of FPJ on clinical isolates of multidrug-resistant Staphylococcus epidermidis strains.

Design: Sixty strains of S. epidermidis isolated from ocular infections were grown in the presence of FPJ, and minimum inhibitory concentration (MIC) was determined by broth and agar dilution methods.

Results: FPJ at 20% had a MIC equal to 100% (MIC100%) on all 60 strains tested. This inhibition of FPJ was confirmed by the growth kinetics of a multidrug-resistant strain exposed to different concentrations of FPJ. Additionally, the antimicrobial activity of FPJ was compared against commercial beverages containing pomegranate: Ocean Spray(®) had a MIC100% at 20%, followed by Del Valle(®) with a MIC15% at 20% concentration only. The beverages Jumex(®) and Sonrisa(®) did not have any antimicrobial activity. FPJ had the highest polyphenol content and antioxidant capacity.

Conclusions: Overall, FPJ had antimicrobial activity, which might be attributed to its high polyphenol content and antioxidant capacity.

No MeSH data available.


Related in: MedlinePlus