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Antibacterial activity of fresh pomegranate juice against clinical strains of Staphylococcus epidermidis.

Betanzos-Cabrera G, Montes-Rubio PY, Fabela-Illescas HE, Belefant-Miller H, Cancino-Diaz JC - Food Nutr Res (2015)

Bottom Line: Additionally, the antimicrobial activity of FPJ was compared against commercial beverages containing pomegranate: Ocean Spray(®) had a MIC100% at 20%, followed by Del Valle(®) with a MIC15% at 20% concentration only.The beverages Jumex(®) and Sonrisa(®) did not have any antimicrobial activity.Overall, FPJ had antimicrobial activity, which might be attributed to its high polyphenol content and antioxidant capacity.

View Article: PubMed Central - PubMed

Affiliation: Área Académica de Nutrición, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Hidalgo, Mexico; gbetanzo@uaeh.edu.mx.

ABSTRACT

Background: Polyphenols have received a great deal of attention due to their biological functions. Pomegranate (Punica granatum L.) is a polyphenol-rich fruit. In the past decade, studies testing the antimicrobial activity of pomegranates almost exclusively used solvent extracts instead of fresh pomegranate juice (FPJ). The use of FPJ instead of solvent extracts would reduce toxicity issues while increasing patient acceptance. We established a model to test FPJ as a natural antimicrobial agent.

Objective: To evaluate the antimicrobial activity of FPJ on clinical isolates of multidrug-resistant Staphylococcus epidermidis strains.

Design: Sixty strains of S. epidermidis isolated from ocular infections were grown in the presence of FPJ, and minimum inhibitory concentration (MIC) was determined by broth and agar dilution methods.

Results: FPJ at 20% had a MIC equal to 100% (MIC100%) on all 60 strains tested. This inhibition of FPJ was confirmed by the growth kinetics of a multidrug-resistant strain exposed to different concentrations of FPJ. Additionally, the antimicrobial activity of FPJ was compared against commercial beverages containing pomegranate: Ocean Spray(®) had a MIC100% at 20%, followed by Del Valle(®) with a MIC15% at 20% concentration only. The beverages Jumex(®) and Sonrisa(®) did not have any antimicrobial activity. FPJ had the highest polyphenol content and antioxidant capacity.

Conclusions: Overall, FPJ had antimicrobial activity, which might be attributed to its high polyphenol content and antioxidant capacity.

No MeSH data available.


Related in: MedlinePlus

Comparison of antimicrobial activity between FPJ and commercial pomegranate-based beverages. An example of the effect of beverage concentration on strains 144 and 2050 (A and B, respectively) in duplicate. Control – Mueller Hinton media without FPJ; Amp – Mueller Hinton media containing 100 µg/mL of ampicillin; FPJ – fresh pomegranate juice; OSY – Ocean Spray®; DVE – Del Valle®; JUX – Jumex®; and SOA – Sonrisa®.
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Figure 0003: Comparison of antimicrobial activity between FPJ and commercial pomegranate-based beverages. An example of the effect of beverage concentration on strains 144 and 2050 (A and B, respectively) in duplicate. Control – Mueller Hinton media without FPJ; Amp – Mueller Hinton media containing 100 µg/mL of ampicillin; FPJ – fresh pomegranate juice; OSY – Ocean Spray®; DVE – Del Valle®; JUX – Jumex®; and SOA – Sonrisa®.

Mentions: Figure 2 shows the antimicrobial activity of FPJ and various pomegranate beverages at 20% on S. epidermidis strains. FPJ and pomegranate beverages at less than 20% concentrations had no inhibitory effect. Both FPJ and Ocean Spray® at 20% inhibited 100% of bacteria (MIC100%=20%), while Del Valle® at 20% only inhibited 15% of the strains. Neither Jumex® nor Sonrisa® showed inhibitory effects at any concentration used (MIC0%=20%). The presence of a beverage, with the exception of Jumex® and Sonrisa®, appeared to reduce the colony size (FPJ=Ocean Spray®>Del Valle®>Jumex®=Sonrisa®) (Fig. 3). It should be noted that adding FPJ or beverages to the culture medium did not alter the pH value (data not shown).


Antibacterial activity of fresh pomegranate juice against clinical strains of Staphylococcus epidermidis.

Betanzos-Cabrera G, Montes-Rubio PY, Fabela-Illescas HE, Belefant-Miller H, Cancino-Diaz JC - Food Nutr Res (2015)

Comparison of antimicrobial activity between FPJ and commercial pomegranate-based beverages. An example of the effect of beverage concentration on strains 144 and 2050 (A and B, respectively) in duplicate. Control – Mueller Hinton media without FPJ; Amp – Mueller Hinton media containing 100 µg/mL of ampicillin; FPJ – fresh pomegranate juice; OSY – Ocean Spray®; DVE – Del Valle®; JUX – Jumex®; and SOA – Sonrisa®.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440876&req=5

Figure 0003: Comparison of antimicrobial activity between FPJ and commercial pomegranate-based beverages. An example of the effect of beverage concentration on strains 144 and 2050 (A and B, respectively) in duplicate. Control – Mueller Hinton media without FPJ; Amp – Mueller Hinton media containing 100 µg/mL of ampicillin; FPJ – fresh pomegranate juice; OSY – Ocean Spray®; DVE – Del Valle®; JUX – Jumex®; and SOA – Sonrisa®.
Mentions: Figure 2 shows the antimicrobial activity of FPJ and various pomegranate beverages at 20% on S. epidermidis strains. FPJ and pomegranate beverages at less than 20% concentrations had no inhibitory effect. Both FPJ and Ocean Spray® at 20% inhibited 100% of bacteria (MIC100%=20%), while Del Valle® at 20% only inhibited 15% of the strains. Neither Jumex® nor Sonrisa® showed inhibitory effects at any concentration used (MIC0%=20%). The presence of a beverage, with the exception of Jumex® and Sonrisa®, appeared to reduce the colony size (FPJ=Ocean Spray®>Del Valle®>Jumex®=Sonrisa®) (Fig. 3). It should be noted that adding FPJ or beverages to the culture medium did not alter the pH value (data not shown).

Bottom Line: Additionally, the antimicrobial activity of FPJ was compared against commercial beverages containing pomegranate: Ocean Spray(®) had a MIC100% at 20%, followed by Del Valle(®) with a MIC15% at 20% concentration only.The beverages Jumex(®) and Sonrisa(®) did not have any antimicrobial activity.Overall, FPJ had antimicrobial activity, which might be attributed to its high polyphenol content and antioxidant capacity.

View Article: PubMed Central - PubMed

Affiliation: Área Académica de Nutrición, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Hidalgo, Mexico; gbetanzo@uaeh.edu.mx.

ABSTRACT

Background: Polyphenols have received a great deal of attention due to their biological functions. Pomegranate (Punica granatum L.) is a polyphenol-rich fruit. In the past decade, studies testing the antimicrobial activity of pomegranates almost exclusively used solvent extracts instead of fresh pomegranate juice (FPJ). The use of FPJ instead of solvent extracts would reduce toxicity issues while increasing patient acceptance. We established a model to test FPJ as a natural antimicrobial agent.

Objective: To evaluate the antimicrobial activity of FPJ on clinical isolates of multidrug-resistant Staphylococcus epidermidis strains.

Design: Sixty strains of S. epidermidis isolated from ocular infections were grown in the presence of FPJ, and minimum inhibitory concentration (MIC) was determined by broth and agar dilution methods.

Results: FPJ at 20% had a MIC equal to 100% (MIC100%) on all 60 strains tested. This inhibition of FPJ was confirmed by the growth kinetics of a multidrug-resistant strain exposed to different concentrations of FPJ. Additionally, the antimicrobial activity of FPJ was compared against commercial beverages containing pomegranate: Ocean Spray(®) had a MIC100% at 20%, followed by Del Valle(®) with a MIC15% at 20% concentration only. The beverages Jumex(®) and Sonrisa(®) did not have any antimicrobial activity. FPJ had the highest polyphenol content and antioxidant capacity.

Conclusions: Overall, FPJ had antimicrobial activity, which might be attributed to its high polyphenol content and antioxidant capacity.

No MeSH data available.


Related in: MedlinePlus