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Antibacterial activity of fresh pomegranate juice against clinical strains of Staphylococcus epidermidis.

Betanzos-Cabrera G, Montes-Rubio PY, Fabela-Illescas HE, Belefant-Miller H, Cancino-Diaz JC - Food Nutr Res (2015)

Bottom Line: Additionally, the antimicrobial activity of FPJ was compared against commercial beverages containing pomegranate: Ocean Spray(®) had a MIC100% at 20%, followed by Del Valle(®) with a MIC15% at 20% concentration only.The beverages Jumex(®) and Sonrisa(®) did not have any antimicrobial activity.Overall, FPJ had antimicrobial activity, which might be attributed to its high polyphenol content and antioxidant capacity.

View Article: PubMed Central - PubMed

Affiliation: Área Académica de Nutrición, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Hidalgo, Mexico; gbetanzo@uaeh.edu.mx.

ABSTRACT

Background: Polyphenols have received a great deal of attention due to their biological functions. Pomegranate (Punica granatum L.) is a polyphenol-rich fruit. In the past decade, studies testing the antimicrobial activity of pomegranates almost exclusively used solvent extracts instead of fresh pomegranate juice (FPJ). The use of FPJ instead of solvent extracts would reduce toxicity issues while increasing patient acceptance. We established a model to test FPJ as a natural antimicrobial agent.

Objective: To evaluate the antimicrobial activity of FPJ on clinical isolates of multidrug-resistant Staphylococcus epidermidis strains.

Design: Sixty strains of S. epidermidis isolated from ocular infections were grown in the presence of FPJ, and minimum inhibitory concentration (MIC) was determined by broth and agar dilution methods.

Results: FPJ at 20% had a MIC equal to 100% (MIC100%) on all 60 strains tested. This inhibition of FPJ was confirmed by the growth kinetics of a multidrug-resistant strain exposed to different concentrations of FPJ. Additionally, the antimicrobial activity of FPJ was compared against commercial beverages containing pomegranate: Ocean Spray(®) had a MIC100% at 20%, followed by Del Valle(®) with a MIC15% at 20% concentration only. The beverages Jumex(®) and Sonrisa(®) did not have any antimicrobial activity. FPJ had the highest polyphenol content and antioxidant capacity.

Conclusions: Overall, FPJ had antimicrobial activity, which might be attributed to its high polyphenol content and antioxidant capacity.

No MeSH data available.


Related in: MedlinePlus

Antimicrobial activity of fresh pomegranate juice (FPJ). The following results are representative of all strains studied. Growth of 16 S. epidermidis strains (in duplicate), (a) unsupplemented, (b) supplemented with 20% FPJ, or (c) supplemented with 100 µg/mL of ampicillin. Note that six strains were resistant to this antibiotic.
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Figure 0001: Antimicrobial activity of fresh pomegranate juice (FPJ). The following results are representative of all strains studied. Growth of 16 S. epidermidis strains (in duplicate), (a) unsupplemented, (b) supplemented with 20% FPJ, or (c) supplemented with 100 µg/mL of ampicillin. Note that six strains were resistant to this antibiotic.

Mentions: Sixty clinical strains of S. epidermidis isolated from ocular infections were tested to observe the antimicrobial activity of FPJ. FPJ at 20% completely inhibited the growth of all 60 strains (Fig. 1a and b). Ampicillin was used as the control in measuring resistance to antimicrobial activity. Figure 1c shows various strains that were resistant to this ampicillin but were sensitive to FPJ at 20%.


Antibacterial activity of fresh pomegranate juice against clinical strains of Staphylococcus epidermidis.

Betanzos-Cabrera G, Montes-Rubio PY, Fabela-Illescas HE, Belefant-Miller H, Cancino-Diaz JC - Food Nutr Res (2015)

Antimicrobial activity of fresh pomegranate juice (FPJ). The following results are representative of all strains studied. Growth of 16 S. epidermidis strains (in duplicate), (a) unsupplemented, (b) supplemented with 20% FPJ, or (c) supplemented with 100 µg/mL of ampicillin. Note that six strains were resistant to this antibiotic.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440876&req=5

Figure 0001: Antimicrobial activity of fresh pomegranate juice (FPJ). The following results are representative of all strains studied. Growth of 16 S. epidermidis strains (in duplicate), (a) unsupplemented, (b) supplemented with 20% FPJ, or (c) supplemented with 100 µg/mL of ampicillin. Note that six strains were resistant to this antibiotic.
Mentions: Sixty clinical strains of S. epidermidis isolated from ocular infections were tested to observe the antimicrobial activity of FPJ. FPJ at 20% completely inhibited the growth of all 60 strains (Fig. 1a and b). Ampicillin was used as the control in measuring resistance to antimicrobial activity. Figure 1c shows various strains that were resistant to this ampicillin but were sensitive to FPJ at 20%.

Bottom Line: Additionally, the antimicrobial activity of FPJ was compared against commercial beverages containing pomegranate: Ocean Spray(®) had a MIC100% at 20%, followed by Del Valle(®) with a MIC15% at 20% concentration only.The beverages Jumex(®) and Sonrisa(®) did not have any antimicrobial activity.Overall, FPJ had antimicrobial activity, which might be attributed to its high polyphenol content and antioxidant capacity.

View Article: PubMed Central - PubMed

Affiliation: Área Académica de Nutrición, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Hidalgo, Mexico; gbetanzo@uaeh.edu.mx.

ABSTRACT

Background: Polyphenols have received a great deal of attention due to their biological functions. Pomegranate (Punica granatum L.) is a polyphenol-rich fruit. In the past decade, studies testing the antimicrobial activity of pomegranates almost exclusively used solvent extracts instead of fresh pomegranate juice (FPJ). The use of FPJ instead of solvent extracts would reduce toxicity issues while increasing patient acceptance. We established a model to test FPJ as a natural antimicrobial agent.

Objective: To evaluate the antimicrobial activity of FPJ on clinical isolates of multidrug-resistant Staphylococcus epidermidis strains.

Design: Sixty strains of S. epidermidis isolated from ocular infections were grown in the presence of FPJ, and minimum inhibitory concentration (MIC) was determined by broth and agar dilution methods.

Results: FPJ at 20% had a MIC equal to 100% (MIC100%) on all 60 strains tested. This inhibition of FPJ was confirmed by the growth kinetics of a multidrug-resistant strain exposed to different concentrations of FPJ. Additionally, the antimicrobial activity of FPJ was compared against commercial beverages containing pomegranate: Ocean Spray(®) had a MIC100% at 20%, followed by Del Valle(®) with a MIC15% at 20% concentration only. The beverages Jumex(®) and Sonrisa(®) did not have any antimicrobial activity. FPJ had the highest polyphenol content and antioxidant capacity.

Conclusions: Overall, FPJ had antimicrobial activity, which might be attributed to its high polyphenol content and antioxidant capacity.

No MeSH data available.


Related in: MedlinePlus