Limits...
Influence of Different Antioxidants on X-Ray Induced DNA Double-Strand Breaks (DSBs) Using γ-H2AX Immunofluorescence Microscopy in a Preliminary Study.

Brand M, Sommer M, Ellmann S, Wuest W, May MS, Eller A, Vogt S, Lell MM, Kuefner MA, Uder M - PLoS ONE (2015)

Bottom Line: For zinc, trolox and lipoic acid regardless of concentration or pre-incubation time, no significant decrease of γ-H2AX-foci was found.However, ß-carotene (15%), selenium (14%), vitamin E (12%), vitamin C (25%), NAC (43%) and Q 10 (18%) led to a significant reduction of γ-H2AX-foci at a pre-incubation time of 1 hour.The combination of different antioxidants did not have an additive effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, University Hospital Erlangen-Nuremberg, Erlangen, Germany.

ABSTRACT

Background: Radiation exposure occurs in X-ray guided interventional procedures or computed tomography (CT) and γ-H2AX-foci are recognized to represent DNA double-strand breaks (DSBs) as a biomarker for radiation induced damage. Antioxidants may reduce the induction of γ-H2AX-foci by binding free radicals. The aim of this study was to establish a dose-effect relationship and a time-effect relationship for the individual antioxidants on DSBs in human blood lymphocytes.

Materials and methods: Blood samples from volunteers were irradiated with 10 mGy before and after pre-incubation with different antioxidants (zinc, trolox, lipoic acid, ß-carotene, selenium, vitamin E, vitamin C, N-acetyl-L-cysteine (NAC) and Q 10). Thereby, different pre-incubation times, concentrations and combinations of drugs were evaluated. For assessment of DSBs, lymphocytes were stained against the phosphorylated histone variant γ-H2AX.

Results: For zinc, trolox and lipoic acid regardless of concentration or pre-incubation time, no significant decrease of γ-H2AX-foci was found. However, ß-carotene (15%), selenium (14%), vitamin E (12%), vitamin C (25%), NAC (43%) and Q 10 (18%) led to a significant reduction of γ-H2AX-foci at a pre-incubation time of 1 hour. The combination of different antioxidants did not have an additive effect.

Conclusion: Antioxidants administered prior to irradiation demonstrated the potential to reduce γ-H2AX-foci in blood lymphocytes.

No MeSH data available.


Related in: MedlinePlus

Influence of different antioxidant combinations.Formation of excess foci/cell (mean ± standard deviation) after irradiation with 10mGy for different antioxidant combinations at a pre-incubation time of 1 hour and after irradiation with 10 mGy. No combination resulted in a significant excess foci reduction in comparison to the effect of the best single antioxidant.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4440758&req=5

pone.0127142.g005: Influence of different antioxidant combinations.Formation of excess foci/cell (mean ± standard deviation) after irradiation with 10mGy for different antioxidant combinations at a pre-incubation time of 1 hour and after irradiation with 10 mGy. No combination resulted in a significant excess foci reduction in comparison to the effect of the best single antioxidant.

Mentions: The combination of different antioxidants (pre-incubation time 1 hour) did not lead to further significant reduction of excess foci using the standard concentration (1 x SC). Thus, the effect of NAC (0.106 excess foci/cell) could not be increased by a simultaneous addition of vitamin C (0.098 excess foci/cell, p = 0.0813), vitamin E (0.103 excess foci/cell, p = 0.1548) or selenium (0.097 excess foci/cell, p = 0.0691). Additionally the effect of vitamin C (0.121 excess foci/cell) could not be increased by an addition of vitamin E (0.115 excess foci /cell, p = 0.1542) or selenium (0.117 excess foci/cell, p = 0.4808). The combination of selenium (0.132 excess foci/cell) and vitamin E (0.140 excess foci/cell) showed no additive effect (0.131 excess foci/cell, p = 0.1679) (Fig 5).


Influence of Different Antioxidants on X-Ray Induced DNA Double-Strand Breaks (DSBs) Using γ-H2AX Immunofluorescence Microscopy in a Preliminary Study.

Brand M, Sommer M, Ellmann S, Wuest W, May MS, Eller A, Vogt S, Lell MM, Kuefner MA, Uder M - PLoS ONE (2015)

Influence of different antioxidant combinations.Formation of excess foci/cell (mean ± standard deviation) after irradiation with 10mGy for different antioxidant combinations at a pre-incubation time of 1 hour and after irradiation with 10 mGy. No combination resulted in a significant excess foci reduction in comparison to the effect of the best single antioxidant.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440758&req=5

pone.0127142.g005: Influence of different antioxidant combinations.Formation of excess foci/cell (mean ± standard deviation) after irradiation with 10mGy for different antioxidant combinations at a pre-incubation time of 1 hour and after irradiation with 10 mGy. No combination resulted in a significant excess foci reduction in comparison to the effect of the best single antioxidant.
Mentions: The combination of different antioxidants (pre-incubation time 1 hour) did not lead to further significant reduction of excess foci using the standard concentration (1 x SC). Thus, the effect of NAC (0.106 excess foci/cell) could not be increased by a simultaneous addition of vitamin C (0.098 excess foci/cell, p = 0.0813), vitamin E (0.103 excess foci/cell, p = 0.1548) or selenium (0.097 excess foci/cell, p = 0.0691). Additionally the effect of vitamin C (0.121 excess foci/cell) could not be increased by an addition of vitamin E (0.115 excess foci /cell, p = 0.1542) or selenium (0.117 excess foci/cell, p = 0.4808). The combination of selenium (0.132 excess foci/cell) and vitamin E (0.140 excess foci/cell) showed no additive effect (0.131 excess foci/cell, p = 0.1679) (Fig 5).

Bottom Line: For zinc, trolox and lipoic acid regardless of concentration or pre-incubation time, no significant decrease of γ-H2AX-foci was found.However, ß-carotene (15%), selenium (14%), vitamin E (12%), vitamin C (25%), NAC (43%) and Q 10 (18%) led to a significant reduction of γ-H2AX-foci at a pre-incubation time of 1 hour.The combination of different antioxidants did not have an additive effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, University Hospital Erlangen-Nuremberg, Erlangen, Germany.

ABSTRACT

Background: Radiation exposure occurs in X-ray guided interventional procedures or computed tomography (CT) and γ-H2AX-foci are recognized to represent DNA double-strand breaks (DSBs) as a biomarker for radiation induced damage. Antioxidants may reduce the induction of γ-H2AX-foci by binding free radicals. The aim of this study was to establish a dose-effect relationship and a time-effect relationship for the individual antioxidants on DSBs in human blood lymphocytes.

Materials and methods: Blood samples from volunteers were irradiated with 10 mGy before and after pre-incubation with different antioxidants (zinc, trolox, lipoic acid, ß-carotene, selenium, vitamin E, vitamin C, N-acetyl-L-cysteine (NAC) and Q 10). Thereby, different pre-incubation times, concentrations and combinations of drugs were evaluated. For assessment of DSBs, lymphocytes were stained against the phosphorylated histone variant γ-H2AX.

Results: For zinc, trolox and lipoic acid regardless of concentration or pre-incubation time, no significant decrease of γ-H2AX-foci was found. However, ß-carotene (15%), selenium (14%), vitamin E (12%), vitamin C (25%), NAC (43%) and Q 10 (18%) led to a significant reduction of γ-H2AX-foci at a pre-incubation time of 1 hour. The combination of different antioxidants did not have an additive effect.

Conclusion: Antioxidants administered prior to irradiation demonstrated the potential to reduce γ-H2AX-foci in blood lymphocytes.

No MeSH data available.


Related in: MedlinePlus