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Structure and Ultrastructure of the Endodermal Region of the Alimentary Tract in the Freshwater Shrimp Neocaridina heteropoda (Crustacea, Malacostraca).

Sonakowska L, Włodarczyk A, Poprawa I, Binkowski M, Śróbka J, Kamińska K, Kszuk-Jendrysik M, Chajec Ł, Zajusz B, Rost-Roszkowska MM - PLoS ONE (2015)

Bottom Line: Our studies showed that the regionalization in the activity of cells occurs along the length of the hepatopancreatic tubules.The role and ultrastructure of all types of epithelial cells are discussed, with the special emphasis on the function of the E-cells, which are the midgut regenerative cells.Additionally, we present the first report on the existence of an intercellular junction that is connected with the E-cells of Crustacea.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Histology and Embryology, University of Silesia, Katowice, Poland.

ABSTRACT
The freshwater shrimp Neocaridina heteropoda (Crustacea, Malacostraca, Decapoda) originates from Asia and is one of the species that is widely available all over the world because it is the most popular shrimp that is bred in aquaria. The structure and the ultrastructure of the midgut have been described using X-ray microtomography, transmission electron microscopy, light and fluorescence microscopes. The endodermal region of the alimentary system in N. heteropoda consists of an intestine and a hepatopancreas. No differences were observed in the structure and ultrastructure of males and females of the shrimp that were examined. The intestine is a tube-shaped organ and the hepatopancreas is composed of two large diverticles that are divided into the blind-end tubules. Hepatopancreatic tubules have three distinct zones - proximal, medial and distal. Among the epithelial cells of the intestine, two types of cells were distinguished - D and E-cells, while three types of cells were observed in the epithelium of the hepatopancreas - F, B and E-cells. Our studies showed that the regionalization in the activity of cells occurs along the length of the hepatopancreatic tubules. The role and ultrastructure of all types of epithelial cells are discussed, with the special emphasis on the function of the E-cells, which are the midgut regenerative cells. Additionally, we present the first report on the existence of an intercellular junction that is connected with the E-cells of Crustacea.

No MeSH data available.


Related in: MedlinePlus

Proximal region of hepatopancreatic tubules in N. heteropoda.(A) Two types of cells were distinguished in the epithelium of the proximal region in hepatopancreatic tubule: type I—fibrillar cells (fc) and type II—storage cells (sc). TEM. Bar = 1.8 µm. (B) Fibrillar cells (fc) and storage cell (sc). TEM. Bar = 2.7 µm. (C) A higher magnification of (B). Fibrillar cells (fc)—a cortical layer with electron-lucent vesicles (v) and storage cell (sc). TEM. Bar = 1.5 µm. (D) The cytoplasm of fibrillar (fc) and storage (sc) cells. TEM. Bar = 0.7 µm. (E) Gap junctions (arrow) and septate junctions (arrowheads). TEM. Bar = 0.5 µm. (F) Fibrillar cells (fc). Basal membrane with small folds (arrows). TEM. Bar = 2.7 µm. (G) Autophagosomes (au) accumulated above the nucleus (n). TEM. Bar = 1.7 µm. Hepatopancreatic lumen (l), microvilli (mv), nucleus (n), basal lamina (bl), smooth septate junctions (arrows), reserve material (rm), cisterns of the rough (RER) and smooth (SER) endoplasmic reticulum, Golgi complexes (d), mitochondria (m), autophagosome (au).
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pone.0126900.g009: Proximal region of hepatopancreatic tubules in N. heteropoda.(A) Two types of cells were distinguished in the epithelium of the proximal region in hepatopancreatic tubule: type I—fibrillar cells (fc) and type II—storage cells (sc). TEM. Bar = 1.8 µm. (B) Fibrillar cells (fc) and storage cell (sc). TEM. Bar = 2.7 µm. (C) A higher magnification of (B). Fibrillar cells (fc)—a cortical layer with electron-lucent vesicles (v) and storage cell (sc). TEM. Bar = 1.5 µm. (D) The cytoplasm of fibrillar (fc) and storage (sc) cells. TEM. Bar = 0.7 µm. (E) Gap junctions (arrow) and septate junctions (arrowheads). TEM. Bar = 0.5 µm. (F) Fibrillar cells (fc). Basal membrane with small folds (arrows). TEM. Bar = 2.7 µm. (G) Autophagosomes (au) accumulated above the nucleus (n). TEM. Bar = 1.7 µm. Hepatopancreatic lumen (l), microvilli (mv), nucleus (n), basal lamina (bl), smooth septate junctions (arrows), reserve material (rm), cisterns of the rough (RER) and smooth (SER) endoplasmic reticulum, Golgi complexes (d), mitochondria (m), autophagosome (au).

Mentions: Two types of cells can be distinguished in the epithelium of the proximal region in the hepatopancreatic tubule—type I (fibrillar cells) and type II (storage cells) (Figs 1E and 9A).


Structure and Ultrastructure of the Endodermal Region of the Alimentary Tract in the Freshwater Shrimp Neocaridina heteropoda (Crustacea, Malacostraca).

Sonakowska L, Włodarczyk A, Poprawa I, Binkowski M, Śróbka J, Kamińska K, Kszuk-Jendrysik M, Chajec Ł, Zajusz B, Rost-Roszkowska MM - PLoS ONE (2015)

Proximal region of hepatopancreatic tubules in N. heteropoda.(A) Two types of cells were distinguished in the epithelium of the proximal region in hepatopancreatic tubule: type I—fibrillar cells (fc) and type II—storage cells (sc). TEM. Bar = 1.8 µm. (B) Fibrillar cells (fc) and storage cell (sc). TEM. Bar = 2.7 µm. (C) A higher magnification of (B). Fibrillar cells (fc)—a cortical layer with electron-lucent vesicles (v) and storage cell (sc). TEM. Bar = 1.5 µm. (D) The cytoplasm of fibrillar (fc) and storage (sc) cells. TEM. Bar = 0.7 µm. (E) Gap junctions (arrow) and septate junctions (arrowheads). TEM. Bar = 0.5 µm. (F) Fibrillar cells (fc). Basal membrane with small folds (arrows). TEM. Bar = 2.7 µm. (G) Autophagosomes (au) accumulated above the nucleus (n). TEM. Bar = 1.7 µm. Hepatopancreatic lumen (l), microvilli (mv), nucleus (n), basal lamina (bl), smooth septate junctions (arrows), reserve material (rm), cisterns of the rough (RER) and smooth (SER) endoplasmic reticulum, Golgi complexes (d), mitochondria (m), autophagosome (au).
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pone.0126900.g009: Proximal region of hepatopancreatic tubules in N. heteropoda.(A) Two types of cells were distinguished in the epithelium of the proximal region in hepatopancreatic tubule: type I—fibrillar cells (fc) and type II—storage cells (sc). TEM. Bar = 1.8 µm. (B) Fibrillar cells (fc) and storage cell (sc). TEM. Bar = 2.7 µm. (C) A higher magnification of (B). Fibrillar cells (fc)—a cortical layer with electron-lucent vesicles (v) and storage cell (sc). TEM. Bar = 1.5 µm. (D) The cytoplasm of fibrillar (fc) and storage (sc) cells. TEM. Bar = 0.7 µm. (E) Gap junctions (arrow) and septate junctions (arrowheads). TEM. Bar = 0.5 µm. (F) Fibrillar cells (fc). Basal membrane with small folds (arrows). TEM. Bar = 2.7 µm. (G) Autophagosomes (au) accumulated above the nucleus (n). TEM. Bar = 1.7 µm. Hepatopancreatic lumen (l), microvilli (mv), nucleus (n), basal lamina (bl), smooth septate junctions (arrows), reserve material (rm), cisterns of the rough (RER) and smooth (SER) endoplasmic reticulum, Golgi complexes (d), mitochondria (m), autophagosome (au).
Mentions: Two types of cells can be distinguished in the epithelium of the proximal region in the hepatopancreatic tubule—type I (fibrillar cells) and type II (storage cells) (Figs 1E and 9A).

Bottom Line: Our studies showed that the regionalization in the activity of cells occurs along the length of the hepatopancreatic tubules.The role and ultrastructure of all types of epithelial cells are discussed, with the special emphasis on the function of the E-cells, which are the midgut regenerative cells.Additionally, we present the first report on the existence of an intercellular junction that is connected with the E-cells of Crustacea.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Histology and Embryology, University of Silesia, Katowice, Poland.

ABSTRACT
The freshwater shrimp Neocaridina heteropoda (Crustacea, Malacostraca, Decapoda) originates from Asia and is one of the species that is widely available all over the world because it is the most popular shrimp that is bred in aquaria. The structure and the ultrastructure of the midgut have been described using X-ray microtomography, transmission electron microscopy, light and fluorescence microscopes. The endodermal region of the alimentary system in N. heteropoda consists of an intestine and a hepatopancreas. No differences were observed in the structure and ultrastructure of males and females of the shrimp that were examined. The intestine is a tube-shaped organ and the hepatopancreas is composed of two large diverticles that are divided into the blind-end tubules. Hepatopancreatic tubules have three distinct zones - proximal, medial and distal. Among the epithelial cells of the intestine, two types of cells were distinguished - D and E-cells, while three types of cells were observed in the epithelium of the hepatopancreas - F, B and E-cells. Our studies showed that the regionalization in the activity of cells occurs along the length of the hepatopancreatic tubules. The role and ultrastructure of all types of epithelial cells are discussed, with the special emphasis on the function of the E-cells, which are the midgut regenerative cells. Additionally, we present the first report on the existence of an intercellular junction that is connected with the E-cells of Crustacea.

No MeSH data available.


Related in: MedlinePlus