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Structure and Ultrastructure of the Endodermal Region of the Alimentary Tract in the Freshwater Shrimp Neocaridina heteropoda (Crustacea, Malacostraca).

Sonakowska L, Włodarczyk A, Poprawa I, Binkowski M, Śróbka J, Kamińska K, Kszuk-Jendrysik M, Chajec Ł, Zajusz B, Rost-Roszkowska MM - PLoS ONE (2015)

Bottom Line: Our studies showed that the regionalization in the activity of cells occurs along the length of the hepatopancreatic tubules.The role and ultrastructure of all types of epithelial cells are discussed, with the special emphasis on the function of the E-cells, which are the midgut regenerative cells.Additionally, we present the first report on the existence of an intercellular junction that is connected with the E-cells of Crustacea.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Histology and Embryology, University of Silesia, Katowice, Poland.

ABSTRACT
The freshwater shrimp Neocaridina heteropoda (Crustacea, Malacostraca, Decapoda) originates from Asia and is one of the species that is widely available all over the world because it is the most popular shrimp that is bred in aquaria. The structure and the ultrastructure of the midgut have been described using X-ray microtomography, transmission electron microscopy, light and fluorescence microscopes. The endodermal region of the alimentary system in N. heteropoda consists of an intestine and a hepatopancreas. No differences were observed in the structure and ultrastructure of males and females of the shrimp that were examined. The intestine is a tube-shaped organ and the hepatopancreas is composed of two large diverticles that are divided into the blind-end tubules. Hepatopancreatic tubules have three distinct zones - proximal, medial and distal. Among the epithelial cells of the intestine, two types of cells were distinguished - D and E-cells, while three types of cells were observed in the epithelium of the hepatopancreas - F, B and E-cells. Our studies showed that the regionalization in the activity of cells occurs along the length of the hepatopancreatic tubules. The role and ultrastructure of all types of epithelial cells are discussed, with the special emphasis on the function of the E-cells, which are the midgut regenerative cells. Additionally, we present the first report on the existence of an intercellular junction that is connected with the E-cells of Crustacea.

No MeSH data available.


Regenerative cells in the intestine of N. heteropoda.(A-C) Regenerative cells (rc) situated between the basal regions of the digestive cells (dc). (A) Light microscopy. Bar = 12 µm. (B) TEM. Bar = 0.76 µm. (C) The cytoplasm of the regenerative cells (rc) poor in organelles. TEM. Bar = 0.6 µm. (D) Dividing regenerative cell (rc), digestive cell (dc). TEM. Bar = 3 µm. Nucleus (n), basal lamina (bl), mitochondria (m), cisterns of the rough endoplasmic reticulum (RER).
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pone.0126900.g004: Regenerative cells in the intestine of N. heteropoda.(A-C) Regenerative cells (rc) situated between the basal regions of the digestive cells (dc). (A) Light microscopy. Bar = 12 µm. (B) TEM. Bar = 0.76 µm. (C) The cytoplasm of the regenerative cells (rc) poor in organelles. TEM. Bar = 0.6 µm. (D) Dividing regenerative cell (rc), digestive cell (dc). TEM. Bar = 3 µm. Nucleus (n), basal lamina (bl), mitochondria (m), cisterns of the rough endoplasmic reticulum (RER).

Mentions: Oval-shaped regenerative cells are situated between the basal regions of the digestive cells (Figs 1E, 4A and 4B) and they are distributed only in the anterior part of the intestine (about ¼ of the length of the intestine) (Figs 2A and 2B, 4A), while the remaining region is devoid of regenerative cells (Fig 2C). They do not contact the midgut lumen. The basal membrane does not form any folds and the cytoplasm is poor in organelles during the interphase (Fig 4B and 4C). Some mitochondria and cisterns of the rough endoplasmic reticulum are distributed around the large oval-shaped nucleus, which shows a high electron-density and is devoid of any distinct patches of heterochromatin (Fig 4B and 4C). Septate junctions were observed between the regenerative and digestive cells. The regenerative cells undergo mitosis (Fig 4D), which was confirmed by the anti-BrdU labeling (Fig 5A) and anti-phosphohistone H3 labeling (Fig 5B). The process of the differentiation of the regenerative cells was not observed.


Structure and Ultrastructure of the Endodermal Region of the Alimentary Tract in the Freshwater Shrimp Neocaridina heteropoda (Crustacea, Malacostraca).

Sonakowska L, Włodarczyk A, Poprawa I, Binkowski M, Śróbka J, Kamińska K, Kszuk-Jendrysik M, Chajec Ł, Zajusz B, Rost-Roszkowska MM - PLoS ONE (2015)

Regenerative cells in the intestine of N. heteropoda.(A-C) Regenerative cells (rc) situated between the basal regions of the digestive cells (dc). (A) Light microscopy. Bar = 12 µm. (B) TEM. Bar = 0.76 µm. (C) The cytoplasm of the regenerative cells (rc) poor in organelles. TEM. Bar = 0.6 µm. (D) Dividing regenerative cell (rc), digestive cell (dc). TEM. Bar = 3 µm. Nucleus (n), basal lamina (bl), mitochondria (m), cisterns of the rough endoplasmic reticulum (RER).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440751&req=5

pone.0126900.g004: Regenerative cells in the intestine of N. heteropoda.(A-C) Regenerative cells (rc) situated between the basal regions of the digestive cells (dc). (A) Light microscopy. Bar = 12 µm. (B) TEM. Bar = 0.76 µm. (C) The cytoplasm of the regenerative cells (rc) poor in organelles. TEM. Bar = 0.6 µm. (D) Dividing regenerative cell (rc), digestive cell (dc). TEM. Bar = 3 µm. Nucleus (n), basal lamina (bl), mitochondria (m), cisterns of the rough endoplasmic reticulum (RER).
Mentions: Oval-shaped regenerative cells are situated between the basal regions of the digestive cells (Figs 1E, 4A and 4B) and they are distributed only in the anterior part of the intestine (about ¼ of the length of the intestine) (Figs 2A and 2B, 4A), while the remaining region is devoid of regenerative cells (Fig 2C). They do not contact the midgut lumen. The basal membrane does not form any folds and the cytoplasm is poor in organelles during the interphase (Fig 4B and 4C). Some mitochondria and cisterns of the rough endoplasmic reticulum are distributed around the large oval-shaped nucleus, which shows a high electron-density and is devoid of any distinct patches of heterochromatin (Fig 4B and 4C). Septate junctions were observed between the regenerative and digestive cells. The regenerative cells undergo mitosis (Fig 4D), which was confirmed by the anti-BrdU labeling (Fig 5A) and anti-phosphohistone H3 labeling (Fig 5B). The process of the differentiation of the regenerative cells was not observed.

Bottom Line: Our studies showed that the regionalization in the activity of cells occurs along the length of the hepatopancreatic tubules.The role and ultrastructure of all types of epithelial cells are discussed, with the special emphasis on the function of the E-cells, which are the midgut regenerative cells.Additionally, we present the first report on the existence of an intercellular junction that is connected with the E-cells of Crustacea.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Histology and Embryology, University of Silesia, Katowice, Poland.

ABSTRACT
The freshwater shrimp Neocaridina heteropoda (Crustacea, Malacostraca, Decapoda) originates from Asia and is one of the species that is widely available all over the world because it is the most popular shrimp that is bred in aquaria. The structure and the ultrastructure of the midgut have been described using X-ray microtomography, transmission electron microscopy, light and fluorescence microscopes. The endodermal region of the alimentary system in N. heteropoda consists of an intestine and a hepatopancreas. No differences were observed in the structure and ultrastructure of males and females of the shrimp that were examined. The intestine is a tube-shaped organ and the hepatopancreas is composed of two large diverticles that are divided into the blind-end tubules. Hepatopancreatic tubules have three distinct zones - proximal, medial and distal. Among the epithelial cells of the intestine, two types of cells were distinguished - D and E-cells, while three types of cells were observed in the epithelium of the hepatopancreas - F, B and E-cells. Our studies showed that the regionalization in the activity of cells occurs along the length of the hepatopancreatic tubules. The role and ultrastructure of all types of epithelial cells are discussed, with the special emphasis on the function of the E-cells, which are the midgut regenerative cells. Additionally, we present the first report on the existence of an intercellular junction that is connected with the E-cells of Crustacea.

No MeSH data available.