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Structure and Ultrastructure of the Endodermal Region of the Alimentary Tract in the Freshwater Shrimp Neocaridina heteropoda (Crustacea, Malacostraca).

Sonakowska L, Włodarczyk A, Poprawa I, Binkowski M, Śróbka J, Kamińska K, Kszuk-Jendrysik M, Chajec Ł, Zajusz B, Rost-Roszkowska MM - PLoS ONE (2015)

Bottom Line: Our studies showed that the regionalization in the activity of cells occurs along the length of the hepatopancreatic tubules.The role and ultrastructure of all types of epithelial cells are discussed, with the special emphasis on the function of the E-cells, which are the midgut regenerative cells.Additionally, we present the first report on the existence of an intercellular junction that is connected with the E-cells of Crustacea.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Histology and Embryology, University of Silesia, Katowice, Poland.

ABSTRACT
The freshwater shrimp Neocaridina heteropoda (Crustacea, Malacostraca, Decapoda) originates from Asia and is one of the species that is widely available all over the world because it is the most popular shrimp that is bred in aquaria. The structure and the ultrastructure of the midgut have been described using X-ray microtomography, transmission electron microscopy, light and fluorescence microscopes. The endodermal region of the alimentary system in N. heteropoda consists of an intestine and a hepatopancreas. No differences were observed in the structure and ultrastructure of males and females of the shrimp that were examined. The intestine is a tube-shaped organ and the hepatopancreas is composed of two large diverticles that are divided into the blind-end tubules. Hepatopancreatic tubules have three distinct zones - proximal, medial and distal. Among the epithelial cells of the intestine, two types of cells were distinguished - D and E-cells, while three types of cells were observed in the epithelium of the hepatopancreas - F, B and E-cells. Our studies showed that the regionalization in the activity of cells occurs along the length of the hepatopancreatic tubules. The role and ultrastructure of all types of epithelial cells are discussed, with the special emphasis on the function of the E-cells, which are the midgut regenerative cells. Additionally, we present the first report on the existence of an intercellular junction that is connected with the E-cells of Crustacea.

No MeSH data available.


Digestive system of Neocaridina heteropoda with the midgut epithelium.(A) Neocaridina heteropoda. XMT. Bar = 2 mm. (B-C) A fragment of the digestive system of N. heteropoda. (B) XMT. Bar = 2 mm. (C) Stereomicroscopy. Bar = 272 µm. (D) The beginning of two diverticles of the hepatopancreas (arrows). Light microscopy. Bar = 28 µm. (E) Diagrammatic representation of epithelia in the intestine and zones of hepatopancreas. Intestine (in), hepatopancreas (hp), stomach (white arrow), midgut lumen (l), intestine epithelium (in), basal lamina (arrowhead).
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pone.0126900.g001: Digestive system of Neocaridina heteropoda with the midgut epithelium.(A) Neocaridina heteropoda. XMT. Bar = 2 mm. (B-C) A fragment of the digestive system of N. heteropoda. (B) XMT. Bar = 2 mm. (C) Stereomicroscopy. Bar = 272 µm. (D) The beginning of two diverticles of the hepatopancreas (arrows). Light microscopy. Bar = 28 µm. (E) Diagrammatic representation of epithelia in the intestine and zones of hepatopancreas. Intestine (in), hepatopancreas (hp), stomach (white arrow), midgut lumen (l), intestine epithelium (in), basal lamina (arrowhead).

Mentions: The midgut of Neocaridina heteropoda (Fig 1A), which is located on the dorsal side of the body (S1 Video), is composed of two distinct parts—an intestine that is tubular in shape and a large lobular hepatopancreas (Fig 1B and 1C). The following description relates to both males and females as no sexual differences were observed.


Structure and Ultrastructure of the Endodermal Region of the Alimentary Tract in the Freshwater Shrimp Neocaridina heteropoda (Crustacea, Malacostraca).

Sonakowska L, Włodarczyk A, Poprawa I, Binkowski M, Śróbka J, Kamińska K, Kszuk-Jendrysik M, Chajec Ł, Zajusz B, Rost-Roszkowska MM - PLoS ONE (2015)

Digestive system of Neocaridina heteropoda with the midgut epithelium.(A) Neocaridina heteropoda. XMT. Bar = 2 mm. (B-C) A fragment of the digestive system of N. heteropoda. (B) XMT. Bar = 2 mm. (C) Stereomicroscopy. Bar = 272 µm. (D) The beginning of two diverticles of the hepatopancreas (arrows). Light microscopy. Bar = 28 µm. (E) Diagrammatic representation of epithelia in the intestine and zones of hepatopancreas. Intestine (in), hepatopancreas (hp), stomach (white arrow), midgut lumen (l), intestine epithelium (in), basal lamina (arrowhead).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440751&req=5

pone.0126900.g001: Digestive system of Neocaridina heteropoda with the midgut epithelium.(A) Neocaridina heteropoda. XMT. Bar = 2 mm. (B-C) A fragment of the digestive system of N. heteropoda. (B) XMT. Bar = 2 mm. (C) Stereomicroscopy. Bar = 272 µm. (D) The beginning of two diverticles of the hepatopancreas (arrows). Light microscopy. Bar = 28 µm. (E) Diagrammatic representation of epithelia in the intestine and zones of hepatopancreas. Intestine (in), hepatopancreas (hp), stomach (white arrow), midgut lumen (l), intestine epithelium (in), basal lamina (arrowhead).
Mentions: The midgut of Neocaridina heteropoda (Fig 1A), which is located on the dorsal side of the body (S1 Video), is composed of two distinct parts—an intestine that is tubular in shape and a large lobular hepatopancreas (Fig 1B and 1C). The following description relates to both males and females as no sexual differences were observed.

Bottom Line: Our studies showed that the regionalization in the activity of cells occurs along the length of the hepatopancreatic tubules.The role and ultrastructure of all types of epithelial cells are discussed, with the special emphasis on the function of the E-cells, which are the midgut regenerative cells.Additionally, we present the first report on the existence of an intercellular junction that is connected with the E-cells of Crustacea.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Histology and Embryology, University of Silesia, Katowice, Poland.

ABSTRACT
The freshwater shrimp Neocaridina heteropoda (Crustacea, Malacostraca, Decapoda) originates from Asia and is one of the species that is widely available all over the world because it is the most popular shrimp that is bred in aquaria. The structure and the ultrastructure of the midgut have been described using X-ray microtomography, transmission electron microscopy, light and fluorescence microscopes. The endodermal region of the alimentary system in N. heteropoda consists of an intestine and a hepatopancreas. No differences were observed in the structure and ultrastructure of males and females of the shrimp that were examined. The intestine is a tube-shaped organ and the hepatopancreas is composed of two large diverticles that are divided into the blind-end tubules. Hepatopancreatic tubules have three distinct zones - proximal, medial and distal. Among the epithelial cells of the intestine, two types of cells were distinguished - D and E-cells, while three types of cells were observed in the epithelium of the hepatopancreas - F, B and E-cells. Our studies showed that the regionalization in the activity of cells occurs along the length of the hepatopancreatic tubules. The role and ultrastructure of all types of epithelial cells are discussed, with the special emphasis on the function of the E-cells, which are the midgut regenerative cells. Additionally, we present the first report on the existence of an intercellular junction that is connected with the E-cells of Crustacea.

No MeSH data available.