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Hydrogen and oxygen stable isotope fractionation in body fluid compartments of dairy cattle according to season, farm, breed, and reproductive stage.

Abeni F, Petrera F, Capelletti M, Dal Prà A, Bontempo L, Tonon A, Camin F - PLoS ONE (2015)

Bottom Line: Blood plasma, urine, faecal water, and milk were sampled and the isotope ratios of H (2H/1H) and O (18O/16O) were determined.Deuterium excess and isotope-fractionation factors were calculated for each passage from plasma to faeces, urine and milk.The different body water turnover between adult lactating cattle and non-lactating heifers was confirmed by the higher isotopic δ for the latter, with a shift in the isotopic equilibrium towards values more distant from those of drinking water.

View Article: PubMed Central - PubMed

Affiliation: Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per Produzioni Foraggere e Lattiero-casearie, via Porcellasco 7, 26100 Cremona, Italy.

ABSTRACT
Environmental temperature affects water turnover and isotope fractionation by causing water evaporation from the body in mammals. This may lead to rearrangement of the water stable isotope equilibrium in body fluids. We propose an approach to detect possible variations in the isotope ratio in different body fluids on the basis of different homoeothermic adaptations in varying reproductive stages. Three different reproductive stages (pregnant heifer, primiparous lactating cow, and pluriparous lactating cow) of two dairy cattle breeds (Italian Friesian and Modenese) were studied in winter and summer. Blood plasma, urine, faecal water, and milk were sampled and the isotope ratios of H (2H/1H) and O (18O/16O) were determined. Deuterium excess and isotope-fractionation factors were calculated for each passage from plasma to faeces, urine and milk. The effects of the season, reproductive stages and breed on δ2H and δ18O were significant in all the fluids, with few exceptions. Deuterium excess was affected by season in all the analysed fluids. The correlations between water isotope measurements in bovine body fluids ranged between 0.6936 (urine-milk) and 0.7848 (urine-plasma) for δ2H, and between 0.8705 (urine-milk) and 0.9602 (plasma-milk) for δ18O. The increase in both isotopic δ values in all body fluids during summer is representative of a condition in which fractionation took place as a consequence of a different ratio between ingested and excreted water, which leads to an increased presence of the heavy isotopes. The different body water turnover between adult lactating cattle and non-lactating heifers was confirmed by the higher isotopic δ for the latter, with a shift in the isotopic equilibrium towards values more distant from those of drinking water.

No MeSH data available.


Sampling design.Italian Friesian pluriparous cows are highlighted in the dotted rectangles as the common type of cows tested in both farms.
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pone.0127391.g002: Sampling design.Italian Friesian pluriparous cows are highlighted in the dotted rectangles as the common type of cows tested in both farms.

Mentions: The presence of pluriparous cows of two different breeds in Farm BS allowed comparison within the farm, highlighting possible differences between a cosmopolitan milk-specialised breed (Holstein type, represented by the Italian Friesian) and a local dual-purpose (milk and meat production) breed (Modenese) (see Fig 2 for sampling design).


Hydrogen and oxygen stable isotope fractionation in body fluid compartments of dairy cattle according to season, farm, breed, and reproductive stage.

Abeni F, Petrera F, Capelletti M, Dal Prà A, Bontempo L, Tonon A, Camin F - PLoS ONE (2015)

Sampling design.Italian Friesian pluriparous cows are highlighted in the dotted rectangles as the common type of cows tested in both farms.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440727&req=5

pone.0127391.g002: Sampling design.Italian Friesian pluriparous cows are highlighted in the dotted rectangles as the common type of cows tested in both farms.
Mentions: The presence of pluriparous cows of two different breeds in Farm BS allowed comparison within the farm, highlighting possible differences between a cosmopolitan milk-specialised breed (Holstein type, represented by the Italian Friesian) and a local dual-purpose (milk and meat production) breed (Modenese) (see Fig 2 for sampling design).

Bottom Line: Blood plasma, urine, faecal water, and milk were sampled and the isotope ratios of H (2H/1H) and O (18O/16O) were determined.Deuterium excess and isotope-fractionation factors were calculated for each passage from plasma to faeces, urine and milk.The different body water turnover between adult lactating cattle and non-lactating heifers was confirmed by the higher isotopic δ for the latter, with a shift in the isotopic equilibrium towards values more distant from those of drinking water.

View Article: PubMed Central - PubMed

Affiliation: Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per Produzioni Foraggere e Lattiero-casearie, via Porcellasco 7, 26100 Cremona, Italy.

ABSTRACT
Environmental temperature affects water turnover and isotope fractionation by causing water evaporation from the body in mammals. This may lead to rearrangement of the water stable isotope equilibrium in body fluids. We propose an approach to detect possible variations in the isotope ratio in different body fluids on the basis of different homoeothermic adaptations in varying reproductive stages. Three different reproductive stages (pregnant heifer, primiparous lactating cow, and pluriparous lactating cow) of two dairy cattle breeds (Italian Friesian and Modenese) were studied in winter and summer. Blood plasma, urine, faecal water, and milk were sampled and the isotope ratios of H (2H/1H) and O (18O/16O) were determined. Deuterium excess and isotope-fractionation factors were calculated for each passage from plasma to faeces, urine and milk. The effects of the season, reproductive stages and breed on δ2H and δ18O were significant in all the fluids, with few exceptions. Deuterium excess was affected by season in all the analysed fluids. The correlations between water isotope measurements in bovine body fluids ranged between 0.6936 (urine-milk) and 0.7848 (urine-plasma) for δ2H, and between 0.8705 (urine-milk) and 0.9602 (plasma-milk) for δ18O. The increase in both isotopic δ values in all body fluids during summer is representative of a condition in which fractionation took place as a consequence of a different ratio between ingested and excreted water, which leads to an increased presence of the heavy isotopes. The different body water turnover between adult lactating cattle and non-lactating heifers was confirmed by the higher isotopic δ for the latter, with a shift in the isotopic equilibrium towards values more distant from those of drinking water.

No MeSH data available.