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Development of apical blebbing in the boar epididymis.

Hughes J, Berger T - PLoS ONE (2015)

Bottom Line: This work quantifies the histological appearance of apical blebbing in the porcine epididymis during development and examines the role of endogenous estrogens in regulating this blebbing.Apical blebbing appears at puberty and increases in a linear manner into sexual maturity suggesting that this blebbing is a mature phenotype.Endogenous estrogen levels were reduced with an aromatase inhibitor but such a reduction did not affect apical blebbing in treated animals compared with their vehicle-treated littermates.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science, University of California Davis, Davis, California, United States of America.

ABSTRACT
Microvesicles are of increasing interest in biology as part of normal function of numerous systems; from the immune system (T cell activation) to implantation of the embryo (invasion of the trophoblasts) and sperm maturation (protein transfer in the epididymis). Yet, the mechanisms involved in the appearance of apical blebbing from healthy cells as part of their normal function remain understudied. Microvesicles are produced via one of two pathways: exocytosis or apical blebbing also termed ectocytosis. This work quantifies the histological appearance of apical blebbing in the porcine epididymis during development and examines the role of endogenous estrogens in regulating this blebbing. Apical blebbing appears at puberty and increases in a linear manner into sexual maturity suggesting that this blebbing is a mature phenotype. Endogenous estrogen levels were reduced with an aromatase inhibitor but such a reduction did not affect apical blebbing in treated animals compared with their vehicle-treated littermates. Epididymal production of apical blebs is a secretion mechanism of functionally mature principal cells regulated by factors other than estradiol.

No MeSH data available.


Related in: MedlinePlus

Apical blebbing in letrozole treated versus control littermates.Bars represent least squares means with SEM from 4 and 5 animals in the caput and corpus epidiymis, respectively. Apical blebbing in caput and corpus epididymis were not altered when estrogens were reduced.
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pone.0126848.g004: Apical blebbing in letrozole treated versus control littermates.Bars represent least squares means with SEM from 4 and 5 animals in the caput and corpus epidiymis, respectively. Apical blebbing in caput and corpus epididymis were not altered when estrogens were reduced.

Mentions: Temporal Development and Estrogenic Influence: At 11 weeks of age, very few caput and corpus epididymal tubule cross-sections were blebbing (present in one of the five animals). The incidence increased at 16 weeks of age, which coincided with the onset of puberty in these animals, confirmed by presence of sperm in the epididymal lumen. At this time point, (and at later time points) the apical blebbing rates in the corpus is numerically higher but not statistically different than the rate in the caput. By 20 weeks of age, approximately half of the cross-sections in the caput and three quarters of the corpus cross-sections had blebs. Apical blebbing increased with age in a linear manner (Figs 2 and 3). Letrozole treatment (reduced estradiol) did not affect blebbing in either the caput or the corpus region (Fig 4).


Development of apical blebbing in the boar epididymis.

Hughes J, Berger T - PLoS ONE (2015)

Apical blebbing in letrozole treated versus control littermates.Bars represent least squares means with SEM from 4 and 5 animals in the caput and corpus epidiymis, respectively. Apical blebbing in caput and corpus epididymis were not altered when estrogens were reduced.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440725&req=5

pone.0126848.g004: Apical blebbing in letrozole treated versus control littermates.Bars represent least squares means with SEM from 4 and 5 animals in the caput and corpus epidiymis, respectively. Apical blebbing in caput and corpus epididymis were not altered when estrogens were reduced.
Mentions: Temporal Development and Estrogenic Influence: At 11 weeks of age, very few caput and corpus epididymal tubule cross-sections were blebbing (present in one of the five animals). The incidence increased at 16 weeks of age, which coincided with the onset of puberty in these animals, confirmed by presence of sperm in the epididymal lumen. At this time point, (and at later time points) the apical blebbing rates in the corpus is numerically higher but not statistically different than the rate in the caput. By 20 weeks of age, approximately half of the cross-sections in the caput and three quarters of the corpus cross-sections had blebs. Apical blebbing increased with age in a linear manner (Figs 2 and 3). Letrozole treatment (reduced estradiol) did not affect blebbing in either the caput or the corpus region (Fig 4).

Bottom Line: This work quantifies the histological appearance of apical blebbing in the porcine epididymis during development and examines the role of endogenous estrogens in regulating this blebbing.Apical blebbing appears at puberty and increases in a linear manner into sexual maturity suggesting that this blebbing is a mature phenotype.Endogenous estrogen levels were reduced with an aromatase inhibitor but such a reduction did not affect apical blebbing in treated animals compared with their vehicle-treated littermates.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science, University of California Davis, Davis, California, United States of America.

ABSTRACT
Microvesicles are of increasing interest in biology as part of normal function of numerous systems; from the immune system (T cell activation) to implantation of the embryo (invasion of the trophoblasts) and sperm maturation (protein transfer in the epididymis). Yet, the mechanisms involved in the appearance of apical blebbing from healthy cells as part of their normal function remain understudied. Microvesicles are produced via one of two pathways: exocytosis or apical blebbing also termed ectocytosis. This work quantifies the histological appearance of apical blebbing in the porcine epididymis during development and examines the role of endogenous estrogens in regulating this blebbing. Apical blebbing appears at puberty and increases in a linear manner into sexual maturity suggesting that this blebbing is a mature phenotype. Endogenous estrogen levels were reduced with an aromatase inhibitor but such a reduction did not affect apical blebbing in treated animals compared with their vehicle-treated littermates. Epididymal production of apical blebs is a secretion mechanism of functionally mature principal cells regulated by factors other than estradiol.

No MeSH data available.


Related in: MedlinePlus