Limits...
Biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes.

Paquette CC, Phanse Y, Perry JL, Sanchez-Vargas I, Airs PM, Dunphy BM, Xu J, Carlson JO, Luft JC, DeSimone JM, Bartholomay LC, Beaty BJ - PLoS Negl Trop Dis (2015)

Bottom Line: Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes.Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract.Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, United States of America.

ABSTRACT

Background: Nanotechnology offers great potential for molecular genetic investigations and potential control of medically important arthropods. Major advances have been made in mammalian systems to define nanoparticle (NP) characteristics that condition trafficking and biodistribution of NPs in the host. Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes.

Methodology/principal findings: PRINT technology was used to construct a library of fluorescently labeled hydrogel NPs of defined size, shape, and surface charge. The biodistribution (organ, tissue, and cell tropisms and trafficking kinetics) of positively and negatively charged 200 nm x 200 nm, 80 nm x 320 nm, and 80 nm x 5000 nm NPs was determined in adult Anopheles gambiae mosquitoes as a function of the route of challenge (ingestion, injection or contact) using whole body imaging and fluorescence microscopy. Mosquitoes readily ingested NPs in sugar solution. Whole body fluorescence imaging revealed substantial NP accumulation (load) in the alimentary tracts of the adult mosquitoes, with the greatest loads in the diverticula, cardia and foregut. Positively and negatively charged NPs differed in their biodistribution and trafficking. Following oral challenge, negatively charged NPs transited the alimentary tract more rapidly than positively charged NPs. Following contact challenge, negatively charged NPs trafficked more efficiently in alimentary tract tissues. Following parenteral challenge, positively and negatively charged NPs differed in tissue tropisms and trafficking in the hemocoel. Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract.

Conclusions/significance: Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control.

No MeSH data available.


Related in: MedlinePlus

Excretion of 80 nm x 320 nm hydrogel nanoparticles from the alimentary tract of Anopheles gambiae females following a 1 d oral challenge.Excretion of negatively charged NPs resulted in larger and brighter fluorescence spots on filter paper lining the cages than excreted positively charged NPs. Bar = 1 μm.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4440717&req=5

pntd.0003745.g009: Excretion of 80 nm x 320 nm hydrogel nanoparticles from the alimentary tract of Anopheles gambiae females following a 1 d oral challenge.Excretion of negatively charged NPs resulted in larger and brighter fluorescence spots on filter paper lining the cages than excreted positively charged NPs. Bar = 1 μm.

Mentions: Following oral challenge, the negatively charged NPs apparently transited the alimentary tract more quickly than positively charged NPs; The NPs were detected in fewer tissues than positively charged particles at 2 d post challenge (Fig 7A). To investigate this, filter papers were placed on the bottom of selected cages of mosquitoes challenged with positively or negatively charged NPs to capture expelled fluids. Fluorescence intensity and sizes of spots of NPs expelled during or shortly after ingestion were typically much greater for the negatively charged NPs (Fig 9). Much smaller fluorescent spots were detected on the papers and netting (S4 Fig), which were likely due to physical tracking of NPs on tarsi or the proboscis from the NP feeding suspensions. In this regard, NPs were associated frequently and sometimes abundantly with tissues in the proboscis (Fig 10B), which could account for the smaller fluorescent spots (S4 Fig).


Biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes.

Paquette CC, Phanse Y, Perry JL, Sanchez-Vargas I, Airs PM, Dunphy BM, Xu J, Carlson JO, Luft JC, DeSimone JM, Bartholomay LC, Beaty BJ - PLoS Negl Trop Dis (2015)

Excretion of 80 nm x 320 nm hydrogel nanoparticles from the alimentary tract of Anopheles gambiae females following a 1 d oral challenge.Excretion of negatively charged NPs resulted in larger and brighter fluorescence spots on filter paper lining the cages than excreted positively charged NPs. Bar = 1 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440717&req=5

pntd.0003745.g009: Excretion of 80 nm x 320 nm hydrogel nanoparticles from the alimentary tract of Anopheles gambiae females following a 1 d oral challenge.Excretion of negatively charged NPs resulted in larger and brighter fluorescence spots on filter paper lining the cages than excreted positively charged NPs. Bar = 1 μm.
Mentions: Following oral challenge, the negatively charged NPs apparently transited the alimentary tract more quickly than positively charged NPs; The NPs were detected in fewer tissues than positively charged particles at 2 d post challenge (Fig 7A). To investigate this, filter papers were placed on the bottom of selected cages of mosquitoes challenged with positively or negatively charged NPs to capture expelled fluids. Fluorescence intensity and sizes of spots of NPs expelled during or shortly after ingestion were typically much greater for the negatively charged NPs (Fig 9). Much smaller fluorescent spots were detected on the papers and netting (S4 Fig), which were likely due to physical tracking of NPs on tarsi or the proboscis from the NP feeding suspensions. In this regard, NPs were associated frequently and sometimes abundantly with tissues in the proboscis (Fig 10B), which could account for the smaller fluorescent spots (S4 Fig).

Bottom Line: Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes.Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract.Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, United States of America.

ABSTRACT

Background: Nanotechnology offers great potential for molecular genetic investigations and potential control of medically important arthropods. Major advances have been made in mammalian systems to define nanoparticle (NP) characteristics that condition trafficking and biodistribution of NPs in the host. Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes.

Methodology/principal findings: PRINT technology was used to construct a library of fluorescently labeled hydrogel NPs of defined size, shape, and surface charge. The biodistribution (organ, tissue, and cell tropisms and trafficking kinetics) of positively and negatively charged 200 nm x 200 nm, 80 nm x 320 nm, and 80 nm x 5000 nm NPs was determined in adult Anopheles gambiae mosquitoes as a function of the route of challenge (ingestion, injection or contact) using whole body imaging and fluorescence microscopy. Mosquitoes readily ingested NPs in sugar solution. Whole body fluorescence imaging revealed substantial NP accumulation (load) in the alimentary tracts of the adult mosquitoes, with the greatest loads in the diverticula, cardia and foregut. Positively and negatively charged NPs differed in their biodistribution and trafficking. Following oral challenge, negatively charged NPs transited the alimentary tract more rapidly than positively charged NPs. Following contact challenge, negatively charged NPs trafficked more efficiently in alimentary tract tissues. Following parenteral challenge, positively and negatively charged NPs differed in tissue tropisms and trafficking in the hemocoel. Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract.

Conclusions/significance: Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control.

No MeSH data available.


Related in: MedlinePlus