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Biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes.

Paquette CC, Phanse Y, Perry JL, Sanchez-Vargas I, Airs PM, Dunphy BM, Xu J, Carlson JO, Luft JC, DeSimone JM, Bartholomay LC, Beaty BJ - PLoS Negl Trop Dis (2015)

Bottom Line: Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes.Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract.Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, United States of America.

ABSTRACT

Background: Nanotechnology offers great potential for molecular genetic investigations and potential control of medically important arthropods. Major advances have been made in mammalian systems to define nanoparticle (NP) characteristics that condition trafficking and biodistribution of NPs in the host. Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes.

Methodology/principal findings: PRINT technology was used to construct a library of fluorescently labeled hydrogel NPs of defined size, shape, and surface charge. The biodistribution (organ, tissue, and cell tropisms and trafficking kinetics) of positively and negatively charged 200 nm x 200 nm, 80 nm x 320 nm, and 80 nm x 5000 nm NPs was determined in adult Anopheles gambiae mosquitoes as a function of the route of challenge (ingestion, injection or contact) using whole body imaging and fluorescence microscopy. Mosquitoes readily ingested NPs in sugar solution. Whole body fluorescence imaging revealed substantial NP accumulation (load) in the alimentary tracts of the adult mosquitoes, with the greatest loads in the diverticula, cardia and foregut. Positively and negatively charged NPs differed in their biodistribution and trafficking. Following oral challenge, negatively charged NPs transited the alimentary tract more rapidly than positively charged NPs. Following contact challenge, negatively charged NPs trafficked more efficiently in alimentary tract tissues. Following parenteral challenge, positively and negatively charged NPs differed in tissue tropisms and trafficking in the hemocoel. Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract.

Conclusions/significance: Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control.

No MeSH data available.


Related in: MedlinePlus

Whole body mean fluorescence intensity (MFI) values of Anopheles gambiae females parenterally challenged with 80nm x 320 nm, 200 nm x 200 nm and 80 nm x 5000 nm hydrogel nanoparticles.Negatively charged NPs consistently produced MFI values that were significantly higher than positively charged NPs.
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pntd.0003745.g004: Whole body mean fluorescence intensity (MFI) values of Anopheles gambiae females parenterally challenged with 80nm x 320 nm, 200 nm x 200 nm and 80 nm x 5000 nm hydrogel nanoparticles.Negatively charged NPs consistently produced MFI values that were significantly higher than positively charged NPs.

Mentions: Whole body image analysis was used to detect and quantify NPs following parenteral and oral challenges. Adults were injected with positively or negatively charged 200 nm x 200 nm NPs (250 μg/mL) or sucrose. At 1 d post challenge, mosquitoes injected with the negatively charged NPs exhibited the greater fluorescent signal (Fig 3, Row 2). The greatest Mean Fluorescence Intensity (MFI) values were also detected in mosquitoes challenged with negatively charged NPs (Fig 4), and the MFI values at day seven were similar to those at the day of challenge, with the exception of the 80 nm x 5000 nm positively charged NPs (Fig 4). The reasons for the dramatic differences in MFI between positively and negatively charged NPs following parenteral challenge (Fig 4) may be attributable to more efficient internalization of the positively charged NPs [38].


Biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes.

Paquette CC, Phanse Y, Perry JL, Sanchez-Vargas I, Airs PM, Dunphy BM, Xu J, Carlson JO, Luft JC, DeSimone JM, Bartholomay LC, Beaty BJ - PLoS Negl Trop Dis (2015)

Whole body mean fluorescence intensity (MFI) values of Anopheles gambiae females parenterally challenged with 80nm x 320 nm, 200 nm x 200 nm and 80 nm x 5000 nm hydrogel nanoparticles.Negatively charged NPs consistently produced MFI values that were significantly higher than positively charged NPs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440717&req=5

pntd.0003745.g004: Whole body mean fluorescence intensity (MFI) values of Anopheles gambiae females parenterally challenged with 80nm x 320 nm, 200 nm x 200 nm and 80 nm x 5000 nm hydrogel nanoparticles.Negatively charged NPs consistently produced MFI values that were significantly higher than positively charged NPs.
Mentions: Whole body image analysis was used to detect and quantify NPs following parenteral and oral challenges. Adults were injected with positively or negatively charged 200 nm x 200 nm NPs (250 μg/mL) or sucrose. At 1 d post challenge, mosquitoes injected with the negatively charged NPs exhibited the greater fluorescent signal (Fig 3, Row 2). The greatest Mean Fluorescence Intensity (MFI) values were also detected in mosquitoes challenged with negatively charged NPs (Fig 4), and the MFI values at day seven were similar to those at the day of challenge, with the exception of the 80 nm x 5000 nm positively charged NPs (Fig 4). The reasons for the dramatic differences in MFI between positively and negatively charged NPs following parenteral challenge (Fig 4) may be attributable to more efficient internalization of the positively charged NPs [38].

Bottom Line: Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes.Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract.Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, United States of America.

ABSTRACT

Background: Nanotechnology offers great potential for molecular genetic investigations and potential control of medically important arthropods. Major advances have been made in mammalian systems to define nanoparticle (NP) characteristics that condition trafficking and biodistribution of NPs in the host. Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes.

Methodology/principal findings: PRINT technology was used to construct a library of fluorescently labeled hydrogel NPs of defined size, shape, and surface charge. The biodistribution (organ, tissue, and cell tropisms and trafficking kinetics) of positively and negatively charged 200 nm x 200 nm, 80 nm x 320 nm, and 80 nm x 5000 nm NPs was determined in adult Anopheles gambiae mosquitoes as a function of the route of challenge (ingestion, injection or contact) using whole body imaging and fluorescence microscopy. Mosquitoes readily ingested NPs in sugar solution. Whole body fluorescence imaging revealed substantial NP accumulation (load) in the alimentary tracts of the adult mosquitoes, with the greatest loads in the diverticula, cardia and foregut. Positively and negatively charged NPs differed in their biodistribution and trafficking. Following oral challenge, negatively charged NPs transited the alimentary tract more rapidly than positively charged NPs. Following contact challenge, negatively charged NPs trafficked more efficiently in alimentary tract tissues. Following parenteral challenge, positively and negatively charged NPs differed in tissue tropisms and trafficking in the hemocoel. Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract.

Conclusions/significance: Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control.

No MeSH data available.


Related in: MedlinePlus