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Biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes.

Paquette CC, Phanse Y, Perry JL, Sanchez-Vargas I, Airs PM, Dunphy BM, Xu J, Carlson JO, Luft JC, DeSimone JM, Bartholomay LC, Beaty BJ - PLoS Negl Trop Dis (2015)

Bottom Line: Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes.Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract.Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, United States of America.

ABSTRACT

Background: Nanotechnology offers great potential for molecular genetic investigations and potential control of medically important arthropods. Major advances have been made in mammalian systems to define nanoparticle (NP) characteristics that condition trafficking and biodistribution of NPs in the host. Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes.

Methodology/principal findings: PRINT technology was used to construct a library of fluorescently labeled hydrogel NPs of defined size, shape, and surface charge. The biodistribution (organ, tissue, and cell tropisms and trafficking kinetics) of positively and negatively charged 200 nm x 200 nm, 80 nm x 320 nm, and 80 nm x 5000 nm NPs was determined in adult Anopheles gambiae mosquitoes as a function of the route of challenge (ingestion, injection or contact) using whole body imaging and fluorescence microscopy. Mosquitoes readily ingested NPs in sugar solution. Whole body fluorescence imaging revealed substantial NP accumulation (load) in the alimentary tracts of the adult mosquitoes, with the greatest loads in the diverticula, cardia and foregut. Positively and negatively charged NPs differed in their biodistribution and trafficking. Following oral challenge, negatively charged NPs transited the alimentary tract more rapidly than positively charged NPs. Following contact challenge, negatively charged NPs trafficked more efficiently in alimentary tract tissues. Following parenteral challenge, positively and negatively charged NPs differed in tissue tropisms and trafficking in the hemocoel. Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract.

Conclusions/significance: Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control.

No MeSH data available.


Related in: MedlinePlus

Anopheles gambaie adult female alimentary tract and dissected principal organ systems.A. Sagittal plane diagram of organ systems in situ in An. gambiae. Illustration by R. Isaì Madriz. B. Dissected alimentary tract from female An. gambiae. prob = proboscis,dr. dv = dorsal diverticulum, v. dv = ventral diverticulum,. car = cardia, for.g = foregut, mid.g = midgut, hin.g = hind gut, Mal.tu = Malpighian tubules, Bar = 500 μM.
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pntd.0003745.g002: Anopheles gambaie adult female alimentary tract and dissected principal organ systems.A. Sagittal plane diagram of organ systems in situ in An. gambiae. Illustration by R. Isaì Madriz. B. Dissected alimentary tract from female An. gambiae. prob = proboscis,dr. dv = dorsal diverticulum, v. dv = ventral diverticulum,. car = cardia, for.g = foregut, mid.g = midgut, hin.g = hind gut, Mal.tu = Malpighian tubules, Bar = 500 μM.

Mentions: For contact challenges, a 69 nL drop of fluorescently labeled 80 nm x 320 nm positively or negatively charged NPs (3.6 μg/mL) in water or NP40 (nonyl phenoxypolyethoxylethanol) (0.5%) was placed on of the head/proboscis, thorax or abdomen of adult female mosquitoes (5 to 7 d post-eclosion) using the Nanoject II injector system. All deliveries were performed using a dissecting microscope using glass needles prepared using a vertical pipette puller (P-30, Sutter Instrument Co.). To determine NP tropisms, mosquito tissues were examined for the presence of fluorescence 1 and 2 d post contact; the alimentary tract was dissected and the thoraces, heads, and abdomens were squashed on glass slides (Fig 2), and assayed for NP biodistribution by fluorescence microscopy.


Biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes.

Paquette CC, Phanse Y, Perry JL, Sanchez-Vargas I, Airs PM, Dunphy BM, Xu J, Carlson JO, Luft JC, DeSimone JM, Bartholomay LC, Beaty BJ - PLoS Negl Trop Dis (2015)

Anopheles gambaie adult female alimentary tract and dissected principal organ systems.A. Sagittal plane diagram of organ systems in situ in An. gambiae. Illustration by R. Isaì Madriz. B. Dissected alimentary tract from female An. gambiae. prob = proboscis,dr. dv = dorsal diverticulum, v. dv = ventral diverticulum,. car = cardia, for.g = foregut, mid.g = midgut, hin.g = hind gut, Mal.tu = Malpighian tubules, Bar = 500 μM.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440717&req=5

pntd.0003745.g002: Anopheles gambaie adult female alimentary tract and dissected principal organ systems.A. Sagittal plane diagram of organ systems in situ in An. gambiae. Illustration by R. Isaì Madriz. B. Dissected alimentary tract from female An. gambiae. prob = proboscis,dr. dv = dorsal diverticulum, v. dv = ventral diverticulum,. car = cardia, for.g = foregut, mid.g = midgut, hin.g = hind gut, Mal.tu = Malpighian tubules, Bar = 500 μM.
Mentions: For contact challenges, a 69 nL drop of fluorescently labeled 80 nm x 320 nm positively or negatively charged NPs (3.6 μg/mL) in water or NP40 (nonyl phenoxypolyethoxylethanol) (0.5%) was placed on of the head/proboscis, thorax or abdomen of adult female mosquitoes (5 to 7 d post-eclosion) using the Nanoject II injector system. All deliveries were performed using a dissecting microscope using glass needles prepared using a vertical pipette puller (P-30, Sutter Instrument Co.). To determine NP tropisms, mosquito tissues were examined for the presence of fluorescence 1 and 2 d post contact; the alimentary tract was dissected and the thoraces, heads, and abdomens were squashed on glass slides (Fig 2), and assayed for NP biodistribution by fluorescence microscopy.

Bottom Line: Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes.Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract.Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, United States of America.

ABSTRACT

Background: Nanotechnology offers great potential for molecular genetic investigations and potential control of medically important arthropods. Major advances have been made in mammalian systems to define nanoparticle (NP) characteristics that condition trafficking and biodistribution of NPs in the host. Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes.

Methodology/principal findings: PRINT technology was used to construct a library of fluorescently labeled hydrogel NPs of defined size, shape, and surface charge. The biodistribution (organ, tissue, and cell tropisms and trafficking kinetics) of positively and negatively charged 200 nm x 200 nm, 80 nm x 320 nm, and 80 nm x 5000 nm NPs was determined in adult Anopheles gambiae mosquitoes as a function of the route of challenge (ingestion, injection or contact) using whole body imaging and fluorescence microscopy. Mosquitoes readily ingested NPs in sugar solution. Whole body fluorescence imaging revealed substantial NP accumulation (load) in the alimentary tracts of the adult mosquitoes, with the greatest loads in the diverticula, cardia and foregut. Positively and negatively charged NPs differed in their biodistribution and trafficking. Following oral challenge, negatively charged NPs transited the alimentary tract more rapidly than positively charged NPs. Following contact challenge, negatively charged NPs trafficked more efficiently in alimentary tract tissues. Following parenteral challenge, positively and negatively charged NPs differed in tissue tropisms and trafficking in the hemocoel. Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract.

Conclusions/significance: Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control.

No MeSH data available.


Related in: MedlinePlus