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Pectin Enhances Bio-Control Efficacy by Inducing Colonization and Secretion of Secondary Metabolites by Bacillus amyloliquefaciens SQY 162 in the Rhizosphere of Tobacco.

Wu K, Fang Z, Guo R, Pan B, Shi W, Yuan S, Guan H, Gong M, Shen B, Shen Q - PLoS ONE (2015)

Bottom Line: These results suggested that pectin might serve as an environmental factor in the stimulation of the biofilm formation of SQY 162.Furthermore, in pot experiments the surfactin production and the population of SQY 162 in the rhizosphere significantly increased with the addition of sucrose or pectin, whereas the abundance of the bacterial pathogen Ralstonia decreased.With increased production of secondary metabolites in the rhizosphere of tobacco by SQY 162 and improved colonization density of SQY 162 in the pectin treatment, the disease incidences of bacterial wilt were efficiently suppressed.

View Article: PubMed Central - PubMed

Affiliation: National Engineering Research Center for Organic-based Fertilizers, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China; Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Key Laboratory of Ministry of Education, College of Energy and Environmental Sciences, Yunnan Normal University, Kunming, 650500, China.

ABSTRACT
Bacillus amyloliquefaciens is a plant-beneficial Gram-positive bacterium involved in suppressing soil-borne pathogens through the secretion of secondary metabolites and high rhizosphere competence. Biofilm formation is regarded as a prerequisite for high rhizosphere competence. In this work, we show that plant extracts affect the chemotaxis and biofilm formation of B. amyloliquefaciens SQY 162 (SQY 162). All carbohydrates tested induced the chemotaxis and biofilm formation of the SQY 162 strain; however, the bacterial growth rate was not influenced by the addition of carbohydrates. A strong chemotactic response and biofilm formation of SQY 162 were both induced by pectin through stimulation of surfactin synthesis and transcriptional expression of biofilm formation related matrix genes. These results suggested that pectin might serve as an environmental factor in the stimulation of the biofilm formation of SQY 162. Furthermore, in pot experiments the surfactin production and the population of SQY 162 in the rhizosphere significantly increased with the addition of sucrose or pectin, whereas the abundance of the bacterial pathogen Ralstonia decreased. With increased production of secondary metabolites in the rhizosphere of tobacco by SQY 162 and improved colonization density of SQY 162 in the pectin treatment, the disease incidences of bacterial wilt were efficiently suppressed. The present study revealed that certain plant extracts might serve as energy sources or environmental cues for SQY 162 to enhance the population density on tobacco root and bio-control efficacy of tobacco bacterial wilt.

No MeSH data available.


Related in: MedlinePlus

Effects of sucrose or pectin treatments on the transcription levels of biofilm related genes (epsD, kinC, yqxM, and sft).LB medium was amended with sucrose (Sucrose) or pectin (Pectin) at a final concentration of 0.5% (w:v).
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pone.0127418.g007: Effects of sucrose or pectin treatments on the transcription levels of biofilm related genes (epsD, kinC, yqxM, and sft).LB medium was amended with sucrose (Sucrose) or pectin (Pectin) at a final concentration of 0.5% (w:v).

Mentions: For the sucrose and pectin treatments, the transcription levels were determined for four genes, epsD, yqxM, sft, and kinC, relative to the control. The results suggested that application of sucrose and pectin both significantly increase the transcription levels of epsD, yqxM and kinC in B. amyloliquefaciens SQY 162. As shown in Fig 7, application of sucrose increased the transcription levels of epsD and yqxM by 6.89- and 3.43-fold, respectively. However, the sft transcription levels showed no significant difference with the addition of sucrose. Furthermore, in contrast to the control, application of pectin significantly increased the transcription levels of kinC, epsD and sft by 26.77-, 8.32- and 5.84-fold, respectively. The results also indicated that addition of pectin had little effect on the yqxM transcription levels.


Pectin Enhances Bio-Control Efficacy by Inducing Colonization and Secretion of Secondary Metabolites by Bacillus amyloliquefaciens SQY 162 in the Rhizosphere of Tobacco.

Wu K, Fang Z, Guo R, Pan B, Shi W, Yuan S, Guan H, Gong M, Shen B, Shen Q - PLoS ONE (2015)

Effects of sucrose or pectin treatments on the transcription levels of biofilm related genes (epsD, kinC, yqxM, and sft).LB medium was amended with sucrose (Sucrose) or pectin (Pectin) at a final concentration of 0.5% (w:v).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440637&req=5

pone.0127418.g007: Effects of sucrose or pectin treatments on the transcription levels of biofilm related genes (epsD, kinC, yqxM, and sft).LB medium was amended with sucrose (Sucrose) or pectin (Pectin) at a final concentration of 0.5% (w:v).
Mentions: For the sucrose and pectin treatments, the transcription levels were determined for four genes, epsD, yqxM, sft, and kinC, relative to the control. The results suggested that application of sucrose and pectin both significantly increase the transcription levels of epsD, yqxM and kinC in B. amyloliquefaciens SQY 162. As shown in Fig 7, application of sucrose increased the transcription levels of epsD and yqxM by 6.89- and 3.43-fold, respectively. However, the sft transcription levels showed no significant difference with the addition of sucrose. Furthermore, in contrast to the control, application of pectin significantly increased the transcription levels of kinC, epsD and sft by 26.77-, 8.32- and 5.84-fold, respectively. The results also indicated that addition of pectin had little effect on the yqxM transcription levels.

Bottom Line: These results suggested that pectin might serve as an environmental factor in the stimulation of the biofilm formation of SQY 162.Furthermore, in pot experiments the surfactin production and the population of SQY 162 in the rhizosphere significantly increased with the addition of sucrose or pectin, whereas the abundance of the bacterial pathogen Ralstonia decreased.With increased production of secondary metabolites in the rhizosphere of tobacco by SQY 162 and improved colonization density of SQY 162 in the pectin treatment, the disease incidences of bacterial wilt were efficiently suppressed.

View Article: PubMed Central - PubMed

Affiliation: National Engineering Research Center for Organic-based Fertilizers, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China; Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Key Laboratory of Ministry of Education, College of Energy and Environmental Sciences, Yunnan Normal University, Kunming, 650500, China.

ABSTRACT
Bacillus amyloliquefaciens is a plant-beneficial Gram-positive bacterium involved in suppressing soil-borne pathogens through the secretion of secondary metabolites and high rhizosphere competence. Biofilm formation is regarded as a prerequisite for high rhizosphere competence. In this work, we show that plant extracts affect the chemotaxis and biofilm formation of B. amyloliquefaciens SQY 162 (SQY 162). All carbohydrates tested induced the chemotaxis and biofilm formation of the SQY 162 strain; however, the bacterial growth rate was not influenced by the addition of carbohydrates. A strong chemotactic response and biofilm formation of SQY 162 were both induced by pectin through stimulation of surfactin synthesis and transcriptional expression of biofilm formation related matrix genes. These results suggested that pectin might serve as an environmental factor in the stimulation of the biofilm formation of SQY 162. Furthermore, in pot experiments the surfactin production and the population of SQY 162 in the rhizosphere significantly increased with the addition of sucrose or pectin, whereas the abundance of the bacterial pathogen Ralstonia decreased. With increased production of secondary metabolites in the rhizosphere of tobacco by SQY 162 and improved colonization density of SQY 162 in the pectin treatment, the disease incidences of bacterial wilt were efficiently suppressed. The present study revealed that certain plant extracts might serve as energy sources or environmental cues for SQY 162 to enhance the population density on tobacco root and bio-control efficacy of tobacco bacterial wilt.

No MeSH data available.


Related in: MedlinePlus