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Pectin Enhances Bio-Control Efficacy by Inducing Colonization and Secretion of Secondary Metabolites by Bacillus amyloliquefaciens SQY 162 in the Rhizosphere of Tobacco.

Wu K, Fang Z, Guo R, Pan B, Shi W, Yuan S, Guan H, Gong M, Shen B, Shen Q - PLoS ONE (2015)

Bottom Line: These results suggested that pectin might serve as an environmental factor in the stimulation of the biofilm formation of SQY 162.Furthermore, in pot experiments the surfactin production and the population of SQY 162 in the rhizosphere significantly increased with the addition of sucrose or pectin, whereas the abundance of the bacterial pathogen Ralstonia decreased.With increased production of secondary metabolites in the rhizosphere of tobacco by SQY 162 and improved colonization density of SQY 162 in the pectin treatment, the disease incidences of bacterial wilt were efficiently suppressed.

View Article: PubMed Central - PubMed

Affiliation: National Engineering Research Center for Organic-based Fertilizers, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China; Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Key Laboratory of Ministry of Education, College of Energy and Environmental Sciences, Yunnan Normal University, Kunming, 650500, China.

ABSTRACT
Bacillus amyloliquefaciens is a plant-beneficial Gram-positive bacterium involved in suppressing soil-borne pathogens through the secretion of secondary metabolites and high rhizosphere competence. Biofilm formation is regarded as a prerequisite for high rhizosphere competence. In this work, we show that plant extracts affect the chemotaxis and biofilm formation of B. amyloliquefaciens SQY 162 (SQY 162). All carbohydrates tested induced the chemotaxis and biofilm formation of the SQY 162 strain; however, the bacterial growth rate was not influenced by the addition of carbohydrates. A strong chemotactic response and biofilm formation of SQY 162 were both induced by pectin through stimulation of surfactin synthesis and transcriptional expression of biofilm formation related matrix genes. These results suggested that pectin might serve as an environmental factor in the stimulation of the biofilm formation of SQY 162. Furthermore, in pot experiments the surfactin production and the population of SQY 162 in the rhizosphere significantly increased with the addition of sucrose or pectin, whereas the abundance of the bacterial pathogen Ralstonia decreased. With increased production of secondary metabolites in the rhizosphere of tobacco by SQY 162 and improved colonization density of SQY 162 in the pectin treatment, the disease incidences of bacterial wilt were efficiently suppressed. The present study revealed that certain plant extracts might serve as energy sources or environmental cues for SQY 162 to enhance the population density on tobacco root and bio-control efficacy of tobacco bacterial wilt.

No MeSH data available.


Related in: MedlinePlus

Effects of different carbohydrates on the biofilm formation of SQY 162.The assays were performed in triplicate and quantified by measuring A570 nm of crystal violet-stained wells rinsed with 80% ethanol and 20% acetone. The different letters above each bar refer to the Duncan’s test, p < 0.05. Control (amended with nothing). LB medium was amended with sucrose (Sucrose), fructose (Fructose), pectin (Pectin), xylan (Xylan), galactose (Galactose), lactose (Lactose), or glycerol (Glycerol) at a final concentration of 0.5% (w:v).
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pone.0127418.g004: Effects of different carbohydrates on the biofilm formation of SQY 162.The assays were performed in triplicate and quantified by measuring A570 nm of crystal violet-stained wells rinsed with 80% ethanol and 20% acetone. The different letters above each bar refer to the Duncan’s test, p < 0.05. Control (amended with nothing). LB medium was amended with sucrose (Sucrose), fructose (Fructose), pectin (Pectin), xylan (Xylan), galactose (Galactose), lactose (Lactose), or glycerol (Glycerol) at a final concentration of 0.5% (w:v).

Mentions: The effects of tobacco root exudates and root extracts on the biofilm formation of SQY 162 are determined. The results indicated that both root exudates and root extracts could induce the biofilm formation of SQY 162 (Fig 3). The effects of carbohydrates on the biofilm formation of SQY 162 were tested using the crystal violet staining method. All the individual carbohydrates induced the biofilm formation of SQY 162, whereas their influence abilities differed (Fig 4). The highest biofilm intensity of SQY 162 was observed in the presence of sucrose, followed by fructose and pectin.


Pectin Enhances Bio-Control Efficacy by Inducing Colonization and Secretion of Secondary Metabolites by Bacillus amyloliquefaciens SQY 162 in the Rhizosphere of Tobacco.

Wu K, Fang Z, Guo R, Pan B, Shi W, Yuan S, Guan H, Gong M, Shen B, Shen Q - PLoS ONE (2015)

Effects of different carbohydrates on the biofilm formation of SQY 162.The assays were performed in triplicate and quantified by measuring A570 nm of crystal violet-stained wells rinsed with 80% ethanol and 20% acetone. The different letters above each bar refer to the Duncan’s test, p < 0.05. Control (amended with nothing). LB medium was amended with sucrose (Sucrose), fructose (Fructose), pectin (Pectin), xylan (Xylan), galactose (Galactose), lactose (Lactose), or glycerol (Glycerol) at a final concentration of 0.5% (w:v).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440637&req=5

pone.0127418.g004: Effects of different carbohydrates on the biofilm formation of SQY 162.The assays were performed in triplicate and quantified by measuring A570 nm of crystal violet-stained wells rinsed with 80% ethanol and 20% acetone. The different letters above each bar refer to the Duncan’s test, p < 0.05. Control (amended with nothing). LB medium was amended with sucrose (Sucrose), fructose (Fructose), pectin (Pectin), xylan (Xylan), galactose (Galactose), lactose (Lactose), or glycerol (Glycerol) at a final concentration of 0.5% (w:v).
Mentions: The effects of tobacco root exudates and root extracts on the biofilm formation of SQY 162 are determined. The results indicated that both root exudates and root extracts could induce the biofilm formation of SQY 162 (Fig 3). The effects of carbohydrates on the biofilm formation of SQY 162 were tested using the crystal violet staining method. All the individual carbohydrates induced the biofilm formation of SQY 162, whereas their influence abilities differed (Fig 4). The highest biofilm intensity of SQY 162 was observed in the presence of sucrose, followed by fructose and pectin.

Bottom Line: These results suggested that pectin might serve as an environmental factor in the stimulation of the biofilm formation of SQY 162.Furthermore, in pot experiments the surfactin production and the population of SQY 162 in the rhizosphere significantly increased with the addition of sucrose or pectin, whereas the abundance of the bacterial pathogen Ralstonia decreased.With increased production of secondary metabolites in the rhizosphere of tobacco by SQY 162 and improved colonization density of SQY 162 in the pectin treatment, the disease incidences of bacterial wilt were efficiently suppressed.

View Article: PubMed Central - PubMed

Affiliation: National Engineering Research Center for Organic-based Fertilizers, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China; Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Key Laboratory of Ministry of Education, College of Energy and Environmental Sciences, Yunnan Normal University, Kunming, 650500, China.

ABSTRACT
Bacillus amyloliquefaciens is a plant-beneficial Gram-positive bacterium involved in suppressing soil-borne pathogens through the secretion of secondary metabolites and high rhizosphere competence. Biofilm formation is regarded as a prerequisite for high rhizosphere competence. In this work, we show that plant extracts affect the chemotaxis and biofilm formation of B. amyloliquefaciens SQY 162 (SQY 162). All carbohydrates tested induced the chemotaxis and biofilm formation of the SQY 162 strain; however, the bacterial growth rate was not influenced by the addition of carbohydrates. A strong chemotactic response and biofilm formation of SQY 162 were both induced by pectin through stimulation of surfactin synthesis and transcriptional expression of biofilm formation related matrix genes. These results suggested that pectin might serve as an environmental factor in the stimulation of the biofilm formation of SQY 162. Furthermore, in pot experiments the surfactin production and the population of SQY 162 in the rhizosphere significantly increased with the addition of sucrose or pectin, whereas the abundance of the bacterial pathogen Ralstonia decreased. With increased production of secondary metabolites in the rhizosphere of tobacco by SQY 162 and improved colonization density of SQY 162 in the pectin treatment, the disease incidences of bacterial wilt were efficiently suppressed. The present study revealed that certain plant extracts might serve as energy sources or environmental cues for SQY 162 to enhance the population density on tobacco root and bio-control efficacy of tobacco bacterial wilt.

No MeSH data available.


Related in: MedlinePlus