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Pectin Enhances Bio-Control Efficacy by Inducing Colonization and Secretion of Secondary Metabolites by Bacillus amyloliquefaciens SQY 162 in the Rhizosphere of Tobacco.

Wu K, Fang Z, Guo R, Pan B, Shi W, Yuan S, Guan H, Gong M, Shen B, Shen Q - PLoS ONE (2015)

Bottom Line: These results suggested that pectin might serve as an environmental factor in the stimulation of the biofilm formation of SQY 162.Furthermore, in pot experiments the surfactin production and the population of SQY 162 in the rhizosphere significantly increased with the addition of sucrose or pectin, whereas the abundance of the bacterial pathogen Ralstonia decreased.With increased production of secondary metabolites in the rhizosphere of tobacco by SQY 162 and improved colonization density of SQY 162 in the pectin treatment, the disease incidences of bacterial wilt were efficiently suppressed.

View Article: PubMed Central - PubMed

Affiliation: National Engineering Research Center for Organic-based Fertilizers, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China; Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Key Laboratory of Ministry of Education, College of Energy and Environmental Sciences, Yunnan Normal University, Kunming, 650500, China.

ABSTRACT
Bacillus amyloliquefaciens is a plant-beneficial Gram-positive bacterium involved in suppressing soil-borne pathogens through the secretion of secondary metabolites and high rhizosphere competence. Biofilm formation is regarded as a prerequisite for high rhizosphere competence. In this work, we show that plant extracts affect the chemotaxis and biofilm formation of B. amyloliquefaciens SQY 162 (SQY 162). All carbohydrates tested induced the chemotaxis and biofilm formation of the SQY 162 strain; however, the bacterial growth rate was not influenced by the addition of carbohydrates. A strong chemotactic response and biofilm formation of SQY 162 were both induced by pectin through stimulation of surfactin synthesis and transcriptional expression of biofilm formation related matrix genes. These results suggested that pectin might serve as an environmental factor in the stimulation of the biofilm formation of SQY 162. Furthermore, in pot experiments the surfactin production and the population of SQY 162 in the rhizosphere significantly increased with the addition of sucrose or pectin, whereas the abundance of the bacterial pathogen Ralstonia decreased. With increased production of secondary metabolites in the rhizosphere of tobacco by SQY 162 and improved colonization density of SQY 162 in the pectin treatment, the disease incidences of bacterial wilt were efficiently suppressed. The present study revealed that certain plant extracts might serve as energy sources or environmental cues for SQY 162 to enhance the population density on tobacco root and bio-control efficacy of tobacco bacterial wilt.

No MeSH data available.


Related in: MedlinePlus

The chemotactic response of SQY 162 to treatment with different carbohydrates.The means and standard errors are shown. The different letters above each bar refer to the Duncan’s test, p < 0.05. Phosphate buffer was amended with sucrose (Sucrose), fructose (Fructose), pectin (Pectin), xylan (Xylan), galactose (Galactose), lactose (Lactose), or glycerol (Glycerol) at a final concentration of 0.5% (w:v).
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pone.0127418.g002: The chemotactic response of SQY 162 to treatment with different carbohydrates.The means and standard errors are shown. The different letters above each bar refer to the Duncan’s test, p < 0.05. Phosphate buffer was amended with sucrose (Sucrose), fructose (Fructose), pectin (Pectin), xylan (Xylan), galactose (Galactose), lactose (Lactose), or glycerol (Glycerol) at a final concentration of 0.5% (w:v).

Mentions: The chemotaxis assay indicated that the effects of attracting SQY 162 differed with root exudates and root extracts. The population density of SQY 162 attracted in the syringe in the KL treatments was higher than that in the K treatments (Fig 1). The attracted population of SQY 162 in the KL treatments was 6.20 log cfu/ml, whereas the attracted number of SQY 162 in the K treatments was 6.03 log cfu/ml. The population densities of SQY 162 in treatments amended with individual carbohydrates were all significantly higher than the control (Fig 2), indicating that all the carbohydrates could efficiently attract the strain. SQY 162 was strongly attracted by sucrose, with as many as 6.62 log cfu/ml observed in the syringe.


Pectin Enhances Bio-Control Efficacy by Inducing Colonization and Secretion of Secondary Metabolites by Bacillus amyloliquefaciens SQY 162 in the Rhizosphere of Tobacco.

Wu K, Fang Z, Guo R, Pan B, Shi W, Yuan S, Guan H, Gong M, Shen B, Shen Q - PLoS ONE (2015)

The chemotactic response of SQY 162 to treatment with different carbohydrates.The means and standard errors are shown. The different letters above each bar refer to the Duncan’s test, p < 0.05. Phosphate buffer was amended with sucrose (Sucrose), fructose (Fructose), pectin (Pectin), xylan (Xylan), galactose (Galactose), lactose (Lactose), or glycerol (Glycerol) at a final concentration of 0.5% (w:v).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440637&req=5

pone.0127418.g002: The chemotactic response of SQY 162 to treatment with different carbohydrates.The means and standard errors are shown. The different letters above each bar refer to the Duncan’s test, p < 0.05. Phosphate buffer was amended with sucrose (Sucrose), fructose (Fructose), pectin (Pectin), xylan (Xylan), galactose (Galactose), lactose (Lactose), or glycerol (Glycerol) at a final concentration of 0.5% (w:v).
Mentions: The chemotaxis assay indicated that the effects of attracting SQY 162 differed with root exudates and root extracts. The population density of SQY 162 attracted in the syringe in the KL treatments was higher than that in the K treatments (Fig 1). The attracted population of SQY 162 in the KL treatments was 6.20 log cfu/ml, whereas the attracted number of SQY 162 in the K treatments was 6.03 log cfu/ml. The population densities of SQY 162 in treatments amended with individual carbohydrates were all significantly higher than the control (Fig 2), indicating that all the carbohydrates could efficiently attract the strain. SQY 162 was strongly attracted by sucrose, with as many as 6.62 log cfu/ml observed in the syringe.

Bottom Line: These results suggested that pectin might serve as an environmental factor in the stimulation of the biofilm formation of SQY 162.Furthermore, in pot experiments the surfactin production and the population of SQY 162 in the rhizosphere significantly increased with the addition of sucrose or pectin, whereas the abundance of the bacterial pathogen Ralstonia decreased.With increased production of secondary metabolites in the rhizosphere of tobacco by SQY 162 and improved colonization density of SQY 162 in the pectin treatment, the disease incidences of bacterial wilt were efficiently suppressed.

View Article: PubMed Central - PubMed

Affiliation: National Engineering Research Center for Organic-based Fertilizers, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China; Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Key Laboratory of Ministry of Education, College of Energy and Environmental Sciences, Yunnan Normal University, Kunming, 650500, China.

ABSTRACT
Bacillus amyloliquefaciens is a plant-beneficial Gram-positive bacterium involved in suppressing soil-borne pathogens through the secretion of secondary metabolites and high rhizosphere competence. Biofilm formation is regarded as a prerequisite for high rhizosphere competence. In this work, we show that plant extracts affect the chemotaxis and biofilm formation of B. amyloliquefaciens SQY 162 (SQY 162). All carbohydrates tested induced the chemotaxis and biofilm formation of the SQY 162 strain; however, the bacterial growth rate was not influenced by the addition of carbohydrates. A strong chemotactic response and biofilm formation of SQY 162 were both induced by pectin through stimulation of surfactin synthesis and transcriptional expression of biofilm formation related matrix genes. These results suggested that pectin might serve as an environmental factor in the stimulation of the biofilm formation of SQY 162. Furthermore, in pot experiments the surfactin production and the population of SQY 162 in the rhizosphere significantly increased with the addition of sucrose or pectin, whereas the abundance of the bacterial pathogen Ralstonia decreased. With increased production of secondary metabolites in the rhizosphere of tobacco by SQY 162 and improved colonization density of SQY 162 in the pectin treatment, the disease incidences of bacterial wilt were efficiently suppressed. The present study revealed that certain plant extracts might serve as energy sources or environmental cues for SQY 162 to enhance the population density on tobacco root and bio-control efficacy of tobacco bacterial wilt.

No MeSH data available.


Related in: MedlinePlus