Limits...
Enhancement of experimental cutaneous leishmaniasis by Leishmania extract: identification of a disease-associated antibody specificity.

Silva VM, de-Araújo CF, Navarro IC, Oliveira PR, Pontes-de-Carvalho L - BMC Res Notes (2015)

Bottom Line: In a previous work, we have found that intravenous injections of L. amazonensis amastigote extract (LaE) potentiated a L. braziliensis infection in BALB/c mice, and that this infection-promoting activity could be inhibited by the addition of protease inhibitors to the extract.In order to detect markers of disease evolution, in the present work we analyzed the specificity of the anti-L. amazonensis antibody response of L. braziliensis-infected BALB/c mice injected intravenously with saline or LaE, supplemented or not with protease inhibitors, by the Western blot technique.A Th2 immune response (IgG1 antibody-producing) against this 116 kDa antigen, therefore, could be associated with susceptibility to severe Leishmania infection.

View Article: PubMed Central - PubMed

Affiliation: Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA, 40296-710, Brazil. vmgoesbr@yahoo.com.br.

ABSTRACT

Background: Both Leishmania braziliensis and Leishmania amazonensis induce cutaneous disease when injected in the skin of BALB/c mice. However, L. amazonensis may also visceralize in that strain of mice, infecting mainly the liver and spleen. In addition, whereas BALB/c mice die with a progressive cutaneous disease when infected by L. amazonensis, the infection by L. braziliensis is spontaneously cured. In a previous work, we have found that intravenous injections of L. amazonensis amastigote extract (LaE) potentiated a L. braziliensis infection in BALB/c mice, and that this infection-promoting activity could be inhibited by the addition of protease inhibitors to the extract.

Methods: In order to detect markers of disease evolution, in the present work we analyzed the specificity of the anti-L. amazonensis antibody response of L. braziliensis-infected BALB/c mice injected intravenously with saline or LaE, supplemented or not with protease inhibitors, by the Western blot technique.

Results: IgG1 antibodies recognizing an antigen with apparent molecular weight of 116 kDa were specifically detected in BALB/c mice that had been turned susceptible to L. braziliensis infection by injections of LaE.

Conclusion: A Th2 immune response (IgG1 antibody-producing) against this 116 kDa antigen, therefore, could be associated with susceptibility to severe Leishmania infection.

Show MeSH

Related in: MedlinePlus

Reactivity against L. amazonensis antigens, as assessed by Western blot, of IgG2a antibodies in the sera of BALB/c mice that had been injected with L. amazonensis extract and infected with L. braziliensis. Sera were from blood samples collected five weeks after infection. The infected mice were treated with saline (Saline; lanes 2 to 6), L. amazonensis extract supplemented with protease inhibitor (LaE + PI, lanes 7 to 11) or unsupplemented L. amazonensis extract (LaE, lanes 12 to 16), as detailed in the Materials and Methods. The result obtained with the serum of a naïve mouse is shown in lane 1. The positions of molecular weight markers are shown on the left of the figure
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4440558&req=5

Fig3: Reactivity against L. amazonensis antigens, as assessed by Western blot, of IgG2a antibodies in the sera of BALB/c mice that had been injected with L. amazonensis extract and infected with L. braziliensis. Sera were from blood samples collected five weeks after infection. The infected mice were treated with saline (Saline; lanes 2 to 6), L. amazonensis extract supplemented with protease inhibitor (LaE + PI, lanes 7 to 11) or unsupplemented L. amazonensis extract (LaE, lanes 12 to 16), as detailed in the Materials and Methods. The result obtained with the serum of a naïve mouse is shown in lane 1. The positions of molecular weight markers are shown on the left of the figure

Mentions: Similar antigens were recognized by IgG2a antibodies of the L. braziliensis-infected BALB/c mice, injected or not with LaE, supplemented or not with protease inhibitors (Fig. 3). Three antigens with apparent molecular weights ranging from 34 to 38 kDa reacted with IgG2a antibodies only from the sera of mice treated with LaE, supplemented or not with protease inhibitors (Fig. 3). No difference in antigen recognition was observed with IgG2a antibodies from animals that received unsupplemented LaE and animals that received LaE supplemented with protease inhibitors (Fig. 3, lanes 7 to 16).Fig. 3


Enhancement of experimental cutaneous leishmaniasis by Leishmania extract: identification of a disease-associated antibody specificity.

Silva VM, de-Araújo CF, Navarro IC, Oliveira PR, Pontes-de-Carvalho L - BMC Res Notes (2015)

Reactivity against L. amazonensis antigens, as assessed by Western blot, of IgG2a antibodies in the sera of BALB/c mice that had been injected with L. amazonensis extract and infected with L. braziliensis. Sera were from blood samples collected five weeks after infection. The infected mice were treated with saline (Saline; lanes 2 to 6), L. amazonensis extract supplemented with protease inhibitor (LaE + PI, lanes 7 to 11) or unsupplemented L. amazonensis extract (LaE, lanes 12 to 16), as detailed in the Materials and Methods. The result obtained with the serum of a naïve mouse is shown in lane 1. The positions of molecular weight markers are shown on the left of the figure
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4440558&req=5

Fig3: Reactivity against L. amazonensis antigens, as assessed by Western blot, of IgG2a antibodies in the sera of BALB/c mice that had been injected with L. amazonensis extract and infected with L. braziliensis. Sera were from blood samples collected five weeks after infection. The infected mice were treated with saline (Saline; lanes 2 to 6), L. amazonensis extract supplemented with protease inhibitor (LaE + PI, lanes 7 to 11) or unsupplemented L. amazonensis extract (LaE, lanes 12 to 16), as detailed in the Materials and Methods. The result obtained with the serum of a naïve mouse is shown in lane 1. The positions of molecular weight markers are shown on the left of the figure
Mentions: Similar antigens were recognized by IgG2a antibodies of the L. braziliensis-infected BALB/c mice, injected or not with LaE, supplemented or not with protease inhibitors (Fig. 3). Three antigens with apparent molecular weights ranging from 34 to 38 kDa reacted with IgG2a antibodies only from the sera of mice treated with LaE, supplemented or not with protease inhibitors (Fig. 3). No difference in antigen recognition was observed with IgG2a antibodies from animals that received unsupplemented LaE and animals that received LaE supplemented with protease inhibitors (Fig. 3, lanes 7 to 16).Fig. 3

Bottom Line: In a previous work, we have found that intravenous injections of L. amazonensis amastigote extract (LaE) potentiated a L. braziliensis infection in BALB/c mice, and that this infection-promoting activity could be inhibited by the addition of protease inhibitors to the extract.In order to detect markers of disease evolution, in the present work we analyzed the specificity of the anti-L. amazonensis antibody response of L. braziliensis-infected BALB/c mice injected intravenously with saline or LaE, supplemented or not with protease inhibitors, by the Western blot technique.A Th2 immune response (IgG1 antibody-producing) against this 116 kDa antigen, therefore, could be associated with susceptibility to severe Leishmania infection.

View Article: PubMed Central - PubMed

Affiliation: Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA, 40296-710, Brazil. vmgoesbr@yahoo.com.br.

ABSTRACT

Background: Both Leishmania braziliensis and Leishmania amazonensis induce cutaneous disease when injected in the skin of BALB/c mice. However, L. amazonensis may also visceralize in that strain of mice, infecting mainly the liver and spleen. In addition, whereas BALB/c mice die with a progressive cutaneous disease when infected by L. amazonensis, the infection by L. braziliensis is spontaneously cured. In a previous work, we have found that intravenous injections of L. amazonensis amastigote extract (LaE) potentiated a L. braziliensis infection in BALB/c mice, and that this infection-promoting activity could be inhibited by the addition of protease inhibitors to the extract.

Methods: In order to detect markers of disease evolution, in the present work we analyzed the specificity of the anti-L. amazonensis antibody response of L. braziliensis-infected BALB/c mice injected intravenously with saline or LaE, supplemented or not with protease inhibitors, by the Western blot technique.

Results: IgG1 antibodies recognizing an antigen with apparent molecular weight of 116 kDa were specifically detected in BALB/c mice that had been turned susceptible to L. braziliensis infection by injections of LaE.

Conclusion: A Th2 immune response (IgG1 antibody-producing) against this 116 kDa antigen, therefore, could be associated with susceptibility to severe Leishmania infection.

Show MeSH
Related in: MedlinePlus