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Enhancement of experimental cutaneous leishmaniasis by Leishmania extract: identification of a disease-associated antibody specificity.

Silva VM, de-Araújo CF, Navarro IC, Oliveira PR, Pontes-de-Carvalho L - BMC Res Notes (2015)

Bottom Line: In a previous work, we have found that intravenous injections of L. amazonensis amastigote extract (LaE) potentiated a L. braziliensis infection in BALB/c mice, and that this infection-promoting activity could be inhibited by the addition of protease inhibitors to the extract.In order to detect markers of disease evolution, in the present work we analyzed the specificity of the anti-L. amazonensis antibody response of L. braziliensis-infected BALB/c mice injected intravenously with saline or LaE, supplemented or not with protease inhibitors, by the Western blot technique.A Th2 immune response (IgG1 antibody-producing) against this 116 kDa antigen, therefore, could be associated with susceptibility to severe Leishmania infection.

View Article: PubMed Central - PubMed

Affiliation: Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA, 40296-710, Brazil. vmgoesbr@yahoo.com.br.

ABSTRACT

Background: Both Leishmania braziliensis and Leishmania amazonensis induce cutaneous disease when injected in the skin of BALB/c mice. However, L. amazonensis may also visceralize in that strain of mice, infecting mainly the liver and spleen. In addition, whereas BALB/c mice die with a progressive cutaneous disease when infected by L. amazonensis, the infection by L. braziliensis is spontaneously cured. In a previous work, we have found that intravenous injections of L. amazonensis amastigote extract (LaE) potentiated a L. braziliensis infection in BALB/c mice, and that this infection-promoting activity could be inhibited by the addition of protease inhibitors to the extract.

Methods: In order to detect markers of disease evolution, in the present work we analyzed the specificity of the anti-L. amazonensis antibody response of L. braziliensis-infected BALB/c mice injected intravenously with saline or LaE, supplemented or not with protease inhibitors, by the Western blot technique.

Results: IgG1 antibodies recognizing an antigen with apparent molecular weight of 116 kDa were specifically detected in BALB/c mice that had been turned susceptible to L. braziliensis infection by injections of LaE.

Conclusion: A Th2 immune response (IgG1 antibody-producing) against this 116 kDa antigen, therefore, could be associated with susceptibility to severe Leishmania infection.

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Related in: MedlinePlus

Reactivity against L. amazonensis antigens, as assessed by Western blot, of IgG1 antibodies in the sera of BALB/c mice that had been injected with L. amazonensis extract and infected with L. braziliensis. The sera were from blood samples collected five weeks after infection. The infected mice were treated with saline (Saline; lanes 2 to 6), L. amazonensis extract supplemented with protease inhibitors (LaE + PI, lanes 7 to 11) or unsupplemented L. amazonensis extract (LaE, lanes 12 to 16), as detailed in the Materials and Methods. The result obtained with the serum of a naïve mouse is shown in lane 1. The positions of molecular weight markers are shown on the left of the figure
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Fig2: Reactivity against L. amazonensis antigens, as assessed by Western blot, of IgG1 antibodies in the sera of BALB/c mice that had been injected with L. amazonensis extract and infected with L. braziliensis. The sera were from blood samples collected five weeks after infection. The infected mice were treated with saline (Saline; lanes 2 to 6), L. amazonensis extract supplemented with protease inhibitors (LaE + PI, lanes 7 to 11) or unsupplemented L. amazonensis extract (LaE, lanes 12 to 16), as detailed in the Materials and Methods. The result obtained with the serum of a naïve mouse is shown in lane 1. The positions of molecular weight markers are shown on the left of the figure

Mentions: One protein with apparent molecular weight of 28 kDa, and three proteins with apparent molecular weights from 45 to 54 kDa were only detected by the IgG1 antibodies from mice injected with LaE, regardless of its supplementation with protease inhibitors (Fig. 2). Additional antigens were recognized only by the sera of the mice treated with the unsupplemented extract. These sera stained from 6 to 12 or more bands (Fig. 2, lanes 12 to 16). On the other hand, the sera of the mice injected with protease inhibitor-supplemented extract specifically stained only 4 or 5 bands (Fig. 2, lanes 7 to 11). An antigen with apparent molecular weight of 116 kDa was recognized by all sera of the mice injected with unsupplemented LaE (Fig. 2, lanes 12 to 16), and by none of the sera from the mice injected with the protease inhibitor-supplemented extract (Fig. 2, lanes 7 to 11). The recognition pattern of the serum from a particular mouse injected with unsupplemented extract (Fig. 2, lane 15) only differed from the recognition patterns of the sera from mice treated with the protease inhibitor-supplemented extract (Fig. 2, lanes 7 to 11) by the recognition of that particular antigen.Fig. 2


Enhancement of experimental cutaneous leishmaniasis by Leishmania extract: identification of a disease-associated antibody specificity.

Silva VM, de-Araújo CF, Navarro IC, Oliveira PR, Pontes-de-Carvalho L - BMC Res Notes (2015)

Reactivity against L. amazonensis antigens, as assessed by Western blot, of IgG1 antibodies in the sera of BALB/c mice that had been injected with L. amazonensis extract and infected with L. braziliensis. The sera were from blood samples collected five weeks after infection. The infected mice were treated with saline (Saline; lanes 2 to 6), L. amazonensis extract supplemented with protease inhibitors (LaE + PI, lanes 7 to 11) or unsupplemented L. amazonensis extract (LaE, lanes 12 to 16), as detailed in the Materials and Methods. The result obtained with the serum of a naïve mouse is shown in lane 1. The positions of molecular weight markers are shown on the left of the figure
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4440558&req=5

Fig2: Reactivity against L. amazonensis antigens, as assessed by Western blot, of IgG1 antibodies in the sera of BALB/c mice that had been injected with L. amazonensis extract and infected with L. braziliensis. The sera were from blood samples collected five weeks after infection. The infected mice were treated with saline (Saline; lanes 2 to 6), L. amazonensis extract supplemented with protease inhibitors (LaE + PI, lanes 7 to 11) or unsupplemented L. amazonensis extract (LaE, lanes 12 to 16), as detailed in the Materials and Methods. The result obtained with the serum of a naïve mouse is shown in lane 1. The positions of molecular weight markers are shown on the left of the figure
Mentions: One protein with apparent molecular weight of 28 kDa, and three proteins with apparent molecular weights from 45 to 54 kDa were only detected by the IgG1 antibodies from mice injected with LaE, regardless of its supplementation with protease inhibitors (Fig. 2). Additional antigens were recognized only by the sera of the mice treated with the unsupplemented extract. These sera stained from 6 to 12 or more bands (Fig. 2, lanes 12 to 16). On the other hand, the sera of the mice injected with protease inhibitor-supplemented extract specifically stained only 4 or 5 bands (Fig. 2, lanes 7 to 11). An antigen with apparent molecular weight of 116 kDa was recognized by all sera of the mice injected with unsupplemented LaE (Fig. 2, lanes 12 to 16), and by none of the sera from the mice injected with the protease inhibitor-supplemented extract (Fig. 2, lanes 7 to 11). The recognition pattern of the serum from a particular mouse injected with unsupplemented extract (Fig. 2, lane 15) only differed from the recognition patterns of the sera from mice treated with the protease inhibitor-supplemented extract (Fig. 2, lanes 7 to 11) by the recognition of that particular antigen.Fig. 2

Bottom Line: In a previous work, we have found that intravenous injections of L. amazonensis amastigote extract (LaE) potentiated a L. braziliensis infection in BALB/c mice, and that this infection-promoting activity could be inhibited by the addition of protease inhibitors to the extract.In order to detect markers of disease evolution, in the present work we analyzed the specificity of the anti-L. amazonensis antibody response of L. braziliensis-infected BALB/c mice injected intravenously with saline or LaE, supplemented or not with protease inhibitors, by the Western blot technique.A Th2 immune response (IgG1 antibody-producing) against this 116 kDa antigen, therefore, could be associated with susceptibility to severe Leishmania infection.

View Article: PubMed Central - PubMed

Affiliation: Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA, 40296-710, Brazil. vmgoesbr@yahoo.com.br.

ABSTRACT

Background: Both Leishmania braziliensis and Leishmania amazonensis induce cutaneous disease when injected in the skin of BALB/c mice. However, L. amazonensis may also visceralize in that strain of mice, infecting mainly the liver and spleen. In addition, whereas BALB/c mice die with a progressive cutaneous disease when infected by L. amazonensis, the infection by L. braziliensis is spontaneously cured. In a previous work, we have found that intravenous injections of L. amazonensis amastigote extract (LaE) potentiated a L. braziliensis infection in BALB/c mice, and that this infection-promoting activity could be inhibited by the addition of protease inhibitors to the extract.

Methods: In order to detect markers of disease evolution, in the present work we analyzed the specificity of the anti-L. amazonensis antibody response of L. braziliensis-infected BALB/c mice injected intravenously with saline or LaE, supplemented or not with protease inhibitors, by the Western blot technique.

Results: IgG1 antibodies recognizing an antigen with apparent molecular weight of 116 kDa were specifically detected in BALB/c mice that had been turned susceptible to L. braziliensis infection by injections of LaE.

Conclusion: A Th2 immune response (IgG1 antibody-producing) against this 116 kDa antigen, therefore, could be associated with susceptibility to severe Leishmania infection.

Show MeSH
Related in: MedlinePlus