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Extrinsic intestinal denervation modulates tumor development in the small intestine of Apc(Min/+) mice.

Liu V, Dietrich A, Kasparek MS, Benhaqi P, Schneider MR, Schemann M, Seeliger H, Kreis ME - J. Exp. Clin. Cancer Res. (2015)

Bottom Line: Chronic intestinal inflammation is associated with increased risk of colorectal cancer.We aimed to study potential extrinsic neuronal modulation of intestinal tumor development in a mouse model.Experiments were performed with male Apc(Min/+) or wild type mice (4 weeks old, body weight approximately 20 g).

View Article: PubMed Central - PubMed

Affiliation: Department of General-, Visceral- and Vascular Surgery, Charité University Medicine, Campus Benjamin Franklin, Hindenburgdamm 30, D-12000, Berlin, Germany. verena.liu@charite.de.

ABSTRACT

Background: Innervation interacts with enteric immune responses. Chronic intestinal inflammation is associated with increased risk of colorectal cancer. We aimed to study potential extrinsic neuronal modulation of intestinal tumor development in a mouse model.

Methods: Experiments were performed with male Apc(Min/+) or wild type mice (4 weeks old, body weight approximately 20 g). Subgroups with subdiaphragmatic vagotomy (apcV/wtV), sympathetic denervation of the small intestine (apcS/wtS) or sham operated controls (apcC/wtC) were investigated (n = 6-14 per group). Three months after surgical manipulation, 10 cm of terminal ileum were excised, fixed for 48 h in 4% paraformaldehyde and all tumors were counted and their area determined in mm(2) (mean ± standard error of the mean (SEM)). Whole mounts of the muscularis of terminal ileum and duodenum (internal positive control) were also stained for tyrosine hydroxylase to confirm successful sympathetic denervation.

Results: Tumor count in Apc(Min/+) mice was 62 ± 8 (apcC), 46 ± 11 (apcV) and 54 ± 8 (apcS) which was increased compared to wildtype controls with 4 ± 0.5 (wtC), 5 ± 0.5 (wtV) and 5 ± 0.6 (wtS; all p < 0.05). For Apc(Min/+) groups, vagotomized animals showed a trend towards decreased tumor counts compared to sham operated Apc(Min/+) controls while sympathetic denervation was similar to sham Apc(Min/+). Area covered by tumors in Apc(Min/+) mice was 55 ± 10 (apcC), 31 ± 8 (apcV) and 42 ± 8 (apcS) mm(2), which was generally increased compared to wildtype controls with 7 ± 0.6 (wtC), 7 ± 0.4 (wtV) and 7 ± 0.6 (wtS) mm(2) (all p < 0.05). In Apc(Min/+) groups, tumor area was decreased in vagotomized animals compared to sham operated controls (p < 0.05) while sympathetically denervated mice showed a minor trend to decreased tumor area compared to controls.

Conclusions: Extrinsic innervation of the small bowel is likely to modulate tumor development in Apc(Min/+) mice. Interrupted vagal innervation, but not sympathetic denervation, seems to inhibit tumor growth.

No MeSH data available.


Related in: MedlinePlus

Ileal tumor count. Number of tumors in a 10 cm segment of terminal ileum in ApcMin/+ mice and wt animals after sham operation, vagotomy and complete mesenteric (sympathetic) denervation. Data are mean ± SEM. P < 0.05 for ApcMin/+ vs. wt (*).
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Fig4: Ileal tumor count. Number of tumors in a 10 cm segment of terminal ileum in ApcMin/+ mice and wt animals after sham operation, vagotomy and complete mesenteric (sympathetic) denervation. Data are mean ± SEM. P < 0.05 for ApcMin/+ vs. wt (*).

Mentions: A dramatic increase of tumor growth was observed in the terminal ileum of ApcMin/+ mice compared to wildtype control animals (Figure 4). In sham operated ApcMin/+ animals 62 ± 8 tumors were counted in the evaluated 10 cm segment of terminal ileum which was increased compared to 4 ± 0.5 tumors in wildtype controls (p < 0.05; Figure 4). Following subdiaphragmatic vagotomy, tumor count was reduced to 46 ± 11 in APCmin/+ mice while tumor count following denervation at the level of the superior mesenteric artery was 54 ± 8 which was not significantly different compared to sham operated ApcMin/+ animals. Tumor counts were 5 ± 0.6 in wildtype controls following subdiaphragmatic vagotomy and 5 ± 0.5 following denervation at the mesenteric artery.Figure 4


Extrinsic intestinal denervation modulates tumor development in the small intestine of Apc(Min/+) mice.

Liu V, Dietrich A, Kasparek MS, Benhaqi P, Schneider MR, Schemann M, Seeliger H, Kreis ME - J. Exp. Clin. Cancer Res. (2015)

Ileal tumor count. Number of tumors in a 10 cm segment of terminal ileum in ApcMin/+ mice and wt animals after sham operation, vagotomy and complete mesenteric (sympathetic) denervation. Data are mean ± SEM. P < 0.05 for ApcMin/+ vs. wt (*).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4440557&req=5

Fig4: Ileal tumor count. Number of tumors in a 10 cm segment of terminal ileum in ApcMin/+ mice and wt animals after sham operation, vagotomy and complete mesenteric (sympathetic) denervation. Data are mean ± SEM. P < 0.05 for ApcMin/+ vs. wt (*).
Mentions: A dramatic increase of tumor growth was observed in the terminal ileum of ApcMin/+ mice compared to wildtype control animals (Figure 4). In sham operated ApcMin/+ animals 62 ± 8 tumors were counted in the evaluated 10 cm segment of terminal ileum which was increased compared to 4 ± 0.5 tumors in wildtype controls (p < 0.05; Figure 4). Following subdiaphragmatic vagotomy, tumor count was reduced to 46 ± 11 in APCmin/+ mice while tumor count following denervation at the level of the superior mesenteric artery was 54 ± 8 which was not significantly different compared to sham operated ApcMin/+ animals. Tumor counts were 5 ± 0.6 in wildtype controls following subdiaphragmatic vagotomy and 5 ± 0.5 following denervation at the mesenteric artery.Figure 4

Bottom Line: Chronic intestinal inflammation is associated with increased risk of colorectal cancer.We aimed to study potential extrinsic neuronal modulation of intestinal tumor development in a mouse model.Experiments were performed with male Apc(Min/+) or wild type mice (4 weeks old, body weight approximately 20 g).

View Article: PubMed Central - PubMed

Affiliation: Department of General-, Visceral- and Vascular Surgery, Charité University Medicine, Campus Benjamin Franklin, Hindenburgdamm 30, D-12000, Berlin, Germany. verena.liu@charite.de.

ABSTRACT

Background: Innervation interacts with enteric immune responses. Chronic intestinal inflammation is associated with increased risk of colorectal cancer. We aimed to study potential extrinsic neuronal modulation of intestinal tumor development in a mouse model.

Methods: Experiments were performed with male Apc(Min/+) or wild type mice (4 weeks old, body weight approximately 20 g). Subgroups with subdiaphragmatic vagotomy (apcV/wtV), sympathetic denervation of the small intestine (apcS/wtS) or sham operated controls (apcC/wtC) were investigated (n = 6-14 per group). Three months after surgical manipulation, 10 cm of terminal ileum were excised, fixed for 48 h in 4% paraformaldehyde and all tumors were counted and their area determined in mm(2) (mean ± standard error of the mean (SEM)). Whole mounts of the muscularis of terminal ileum and duodenum (internal positive control) were also stained for tyrosine hydroxylase to confirm successful sympathetic denervation.

Results: Tumor count in Apc(Min/+) mice was 62 ± 8 (apcC), 46 ± 11 (apcV) and 54 ± 8 (apcS) which was increased compared to wildtype controls with 4 ± 0.5 (wtC), 5 ± 0.5 (wtV) and 5 ± 0.6 (wtS; all p < 0.05). For Apc(Min/+) groups, vagotomized animals showed a trend towards decreased tumor counts compared to sham operated Apc(Min/+) controls while sympathetic denervation was similar to sham Apc(Min/+). Area covered by tumors in Apc(Min/+) mice was 55 ± 10 (apcC), 31 ± 8 (apcV) and 42 ± 8 (apcS) mm(2), which was generally increased compared to wildtype controls with 7 ± 0.6 (wtC), 7 ± 0.4 (wtV) and 7 ± 0.6 (wtS) mm(2) (all p < 0.05). In Apc(Min/+) groups, tumor area was decreased in vagotomized animals compared to sham operated controls (p < 0.05) while sympathetically denervated mice showed a minor trend to decreased tumor area compared to controls.

Conclusions: Extrinsic innervation of the small bowel is likely to modulate tumor development in Apc(Min/+) mice. Interrupted vagal innervation, but not sympathetic denervation, seems to inhibit tumor growth.

No MeSH data available.


Related in: MedlinePlus