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Roles of small RNAs in the effects of nutrition on apoptosis and spermatogenesis in the adult testis.

Guan Y, Liang G, Hawken PA, Malecki IA, Cozens G, Vercoe PE, Martin GB, Guan le L - Sci Rep (2015)

Bottom Line: Groups of 8 male sheep were fed for a 10% increase or 10% decrease in body mass over 65 days.Novel-miR-144 (miR-98), was found to target three apoptotic genes (TP53, CASP3, FASL).The proportion of miRNAs as a total of small RNAs was greater in well-fed males than in underfed males (P < 0.05) and was correlated (r = 0.8, P < 0.05) with the proportion of piRNAs in well-fed and underfed males.

View Article: PubMed Central - PubMed

Affiliation: UWA Institute of Agriculture and School of Animal Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009.

ABSTRACT
We tested whether reductions in spermatozoal quality induced by under-nutrition are associated with increased germ cell apoptosis and disrupted spermatogenesis, and whether these effects are mediated by small RNAs. Groups of 8 male sheep were fed for a 10% increase or 10% decrease in body mass over 65 days. Underfeeding increased the number of apoptotic germ cells (P < 0.05) and increased the expression of apoptosis-related genes (P < 0.05) in testicular tissue. We identified 44 miRNAs and 35 putative piRNAs that were differentially expressed in well-fed and underfed males (FDR < 0.05). Some were related to reproductive system development, apoptosis (miRNAs), and sperm production and quality (piRNAs). Novel-miR-144 (miR-98), was found to target three apoptotic genes (TP53, CASP3, FASL). The proportion of miRNAs as a total of small RNAs was greater in well-fed males than in underfed males (P < 0.05) and was correlated (r = 0.8, P < 0.05) with the proportion of piRNAs in well-fed and underfed males. In conclusion, the reductions in spermatozoal quality induced by under-nutrition are caused, at least partly, by disruptions to Sertoli cell function and increased germ cell apoptosis, mediated by changes in the expression of miRNAs and piRNAs.

No MeSH data available.


Related in: MedlinePlus

TUNEL-positive cells (arrows) detected in the testis in sexually mature sheep fed the High diet (A) or the Low diet (B) Negative control without terminal deoxynucleotidyl transferase (C) The scale bar represents 50 μm. (D, E, F): relative mRNA expression for apoptosis-related genes, normalized to GAPDH. Values are mean ± SE, N = 8 for each treatment. Significant effect of diet: *P < 0.05.
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f1: TUNEL-positive cells (arrows) detected in the testis in sexually mature sheep fed the High diet (A) or the Low diet (B) Negative control without terminal deoxynucleotidyl transferase (C) The scale bar represents 50 μm. (D, E, F): relative mRNA expression for apoptosis-related genes, normalized to GAPDH. Values are mean ± SE, N = 8 for each treatment. Significant effect of diet: *P < 0.05.

Mentions: TUNEL-positive germ cells were observed in all treatments (Fig. 1A,B), but most were seen in the early stages of spermatogenesis (spermatogonia and spermatocytes) and none were seen amongst spermatids or spermatozoa. The number of TUNEL-positive germ cells per tubule was greater in underfed (1.4 ± 0.3) than in well-fed males (0.49 ± 0.06; P < 0.05). A relationship between under-nutrition and apoptosis was further supported by the expression of the apoptosis-related genes, FASL, TP53 and CASP3, with all three showing greater expression in underfed sheep than well-fed sheep (P < 0.05, Figs. 1D,E,F).


Roles of small RNAs in the effects of nutrition on apoptosis and spermatogenesis in the adult testis.

Guan Y, Liang G, Hawken PA, Malecki IA, Cozens G, Vercoe PE, Martin GB, Guan le L - Sci Rep (2015)

TUNEL-positive cells (arrows) detected in the testis in sexually mature sheep fed the High diet (A) or the Low diet (B) Negative control without terminal deoxynucleotidyl transferase (C) The scale bar represents 50 μm. (D, E, F): relative mRNA expression for apoptosis-related genes, normalized to GAPDH. Values are mean ± SE, N = 8 for each treatment. Significant effect of diet: *P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4440528&req=5

f1: TUNEL-positive cells (arrows) detected in the testis in sexually mature sheep fed the High diet (A) or the Low diet (B) Negative control without terminal deoxynucleotidyl transferase (C) The scale bar represents 50 μm. (D, E, F): relative mRNA expression for apoptosis-related genes, normalized to GAPDH. Values are mean ± SE, N = 8 for each treatment. Significant effect of diet: *P < 0.05.
Mentions: TUNEL-positive germ cells were observed in all treatments (Fig. 1A,B), but most were seen in the early stages of spermatogenesis (spermatogonia and spermatocytes) and none were seen amongst spermatids or spermatozoa. The number of TUNEL-positive germ cells per tubule was greater in underfed (1.4 ± 0.3) than in well-fed males (0.49 ± 0.06; P < 0.05). A relationship between under-nutrition and apoptosis was further supported by the expression of the apoptosis-related genes, FASL, TP53 and CASP3, with all three showing greater expression in underfed sheep than well-fed sheep (P < 0.05, Figs. 1D,E,F).

Bottom Line: Groups of 8 male sheep were fed for a 10% increase or 10% decrease in body mass over 65 days.Novel-miR-144 (miR-98), was found to target three apoptotic genes (TP53, CASP3, FASL).The proportion of miRNAs as a total of small RNAs was greater in well-fed males than in underfed males (P < 0.05) and was correlated (r = 0.8, P < 0.05) with the proportion of piRNAs in well-fed and underfed males.

View Article: PubMed Central - PubMed

Affiliation: UWA Institute of Agriculture and School of Animal Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009.

ABSTRACT
We tested whether reductions in spermatozoal quality induced by under-nutrition are associated with increased germ cell apoptosis and disrupted spermatogenesis, and whether these effects are mediated by small RNAs. Groups of 8 male sheep were fed for a 10% increase or 10% decrease in body mass over 65 days. Underfeeding increased the number of apoptotic germ cells (P < 0.05) and increased the expression of apoptosis-related genes (P < 0.05) in testicular tissue. We identified 44 miRNAs and 35 putative piRNAs that were differentially expressed in well-fed and underfed males (FDR < 0.05). Some were related to reproductive system development, apoptosis (miRNAs), and sperm production and quality (piRNAs). Novel-miR-144 (miR-98), was found to target three apoptotic genes (TP53, CASP3, FASL). The proportion of miRNAs as a total of small RNAs was greater in well-fed males than in underfed males (P < 0.05) and was correlated (r = 0.8, P < 0.05) with the proportion of piRNAs in well-fed and underfed males. In conclusion, the reductions in spermatozoal quality induced by under-nutrition are caused, at least partly, by disruptions to Sertoli cell function and increased germ cell apoptosis, mediated by changes in the expression of miRNAs and piRNAs.

No MeSH data available.


Related in: MedlinePlus